Mutation of an N-terminal acidic-rich region of p115-RhoGEF dissociates alpha13 binding and alpha13-promoted plasma membrane recruitment.

abstract：:The H-Ras-like suppressor (HRASLS) is a protein family consisting of five members in humans. Despite their discovery as tumor suppressors, we demonstrated that all these proteins are phospholipid-metabolizing enzymes, such as phospholipase (PL) A1 /A2 and acyltransferase. We thus proposed to rename HRASLS1-5 as PLA/ac...

journal_title：FEBS letters

authors： Uyama T,Tsuboi K,Ueda N

更新日期：2017-09-01 00:00:00

abstract：:Lysophospholipids are liberated during venomous action. In this study we demonstrated that lysophosphatidyl choline (LPC) of various acyl chains enhances considerably the activity of snake venom phosphodiesterase (PDE). Lysophosphatidic acid (LPA) and its cyclic form (cLPA), on the other hand, were found to inhibit th...

journal_title：FEBS letters

authors： Mamillapalli R,Haimovitz R,Ohad M,Shinitzky M

更新日期：1998-10-02 00:00:00

abstract：:We report that the rat beta 3-adrenergic receptor (beta 3-AR) gene has an intron. The intron starts with an in-frame stop codon with the result that unspliced transcripts will encode a C-terminal truncated protein. The reported protein sequences of mouse and human beta 3-AR were both deduced from genomic DNA sequences...

journal_title：FEBS letters

authors： Bensaid M,Kaghad M,Rodriguez M,Le Fur G,Caput D

更新日期：1993-03-08 00:00:00

abstract：:Bone morphogenetic proteins (BMPs) are members of the transforming growth factor-β (TGF-β) family that signal via type I and type II serine/threonine kinase receptors and intracellular Smad transcription factors. BMPs are multifunctional regulators of development and tissue homeostasis and they were initially characte...

journal_title：FEBS letters

authors： Cai J,Pardali E,Sánchez-Duffhues G,ten Dijke P

更新日期：2012-07-04 00:00:00

abstract：:The effects of protein disulfide isomerase (PDI) on the four structured des species that accumulate in the rate-determining steps of ribonuclease A folding were investigated at pH 8.0 and 15 degrees C. The results indicate that PDI catalyzes the conversion of the kinetically trapped intermediates, des-[26-84] and des-...

journal_title：FEBS letters

authors： Shin HC,Song MC,Scheraga HA

更新日期：2002-06-19 00:00:00

abstract：:Mediatophore is a protein purified from the nerve terminal membranes of Torpedo electric organ. It confers to artificial membranes a calcium-dependent mechanism that translocates acetylcholine. When similar reconstitution experiments are applied to rat brain synaptosomal membranes they reveal the presence of mediatoph...

journal_title：FEBS letters

authors： Israël M,Lesbats B,Morel N,Manaranche R,Le Gal la Salle G

更新日期：1988-06-20 00:00:00

abstract：:It is increasingly clear that microRNAs play a crucial role in tumorigenesis. Recently, emerging evidence suggested that miR-26a is aberrantly expressed in tumor tissues. In our study, frequent down-regulation of miR-26a was observed in 10 human bladder cancer tissues. Forced expression of miR-26a in the bladder cance...

journal_title：FEBS letters

authors： Lin Y,Chen H,Hu Z,Mao Y,Xu X,Zhu Y,Xu X,Wu J,Li S,Mao Q,Zheng X,Xie L

更新日期：2013-08-02 00:00:00

abstract：:We developed a novel method to quantitatively measure GTP gamma S binding to specific G proteins in crude membranes using G-protein antibodies. The basic strategy was that the materials were initially incubated with [35S]GTP gamma S at 37 degrees C. After 4 degrees C incubation in the wells of an ELISA plate precoated...

journal_title：FEBS letters

authors： Okamoto T,Ikezu T,Murayama Y,Ogata E,Nishimoto I

更新日期：1992-06-29 00:00:00

abstract：:The binding of L-[35S]methionine in vivo labelled CryIC toxin to its receptor in brush border membrane vesicle (BBMV's) prepared from Spodoptera littoralis and Bombyx mori was studied. Both insect species were highly susceptible to the CryIC toxin in bioassays, B. mori being 7-fold more sensitive to CryIC than S. litt...

journal_title：FEBS letters

authors： Sanchis V,Chaufaux J,Pauron D

更新日期：1994-10-24 00:00:00

abstract：:Granulocyte colony-stimulating factor (G-CSF) has been recently shown to induce the high-affinity Fc receptor for IgG (Fc(gammaRI)/CD64) in human polymorphonuclear neutrophils (PMN). To elucidate the molecular mechanisms whereby G-CSF exerts this effect, we examined whether the cytokine induces the binding of transcri...

journal_title：FEBS letters

authors： Bovolenta C,Gasperini S,Cassatella MA

更新日期：1996-05-20 00:00:00

abstract：:Fibroblasts were exposed to various concentrations of hydrogen peroxide and the removal of oxidized proteins was followed by determining protein-bound carbonyls. Fibroblasts are able to increase the turnover of metabolically radiolabeled proteins after treatment with hydrogen peroxide. It was demonstrated for the firs...

journal_title：FEBS letters

authors： Sitte N,Merker K,Grune T

更新日期：1998-12-04 00:00:00

abstract：:An essential component of the chromatin remodeling machinery is NURF (Nucleosome Remodeling Factor), the founding member of the ISWI family of chromatin remodeling complexes. In vertebrates and invertebrates alike, NURF has many important functions in chromatin biology including regulating transcription, establishing ...

journal_title：FEBS letters

authors： Alkhatib SG,Landry JW

更新日期：2011-10-20 00:00:00

abstract：:Blebs of the sarcoplasmic reticulum (SR) membrane of heart muscle cells were generated after saponin perforation of the plasma membrane followed by complete hypercontraction of the cell. Although characteristic proteins of the plasma membrane, namely the beta1-adrenoreceptor and Galphai, were stained by monoclonal ant...

journal_title：FEBS letters

authors： Böhle T,Brandt MC,Henn N,Schmidt A,Bloch W,Beuckelmann DJ

更新日期：2001-09-21 00:00:00

abstract：:Reaction of ubiquinone in the high-affinity quinone-binding site (QH) in bo-type ubiquinol oxidase from Escherichia coli was revealed by EPR and optical studies. In the QH site, ubiquinol was shown to be oxidized to ubisemiquinone and to ubiquinone, while no semiquinone signal was detected in the oxidase isolated from...

journal_title：FEBS letters

authors： Sato-Watanabe M,Itoh S,Mogi T,Matsuura K,Miyoshi H,Anraku Y

更新日期：1995-10-30 00:00:00

abstract：:We report here the isolation and sequence determination of a gene closely linked to the Astyanax red visual pigment gene. Reverse transcription polymerase chain reaction assays show that this new gene (B23 Af) and the previously characterized red and green visual pigment genes of Astyanax are all expressed in the eye....

journal_title：FEBS letters

authors： Yokoyama R,Yokoyama S

更新日期：1993-11-08 00:00:00

abstract：:14-3-3 proteins were the first signaling molecules to be identified as discrete phosphoserine/threonine binding modules. This family of proteins, which includes seven isotypes in human cells and up to 15 in plants, plays critical roles in cell signaling events that control progress through the cell cycle, transcriptio...

journal_title：FEBS letters

authors： Yaffe MB

更新日期：2002-02-20 00:00:00

abstract：:Placentas of mice lacking p57(Kip2) expression have trophoblastic hyperplasia. To elucidate the mechanism underlying this phenomenon, we studied expression of two angiogenic factors, vascular endothelial growth factor (VEGF) and placenta growth factor (PlGF). Immunohistochemical analysis with anti-VEGF antibodies indi...

journal_title：FEBS letters

authors： Matsuura T,Takahashi K,Nakayama K,Kobayashi T,Choi-Miura NH,Tomita M,Kanayama N

更新日期：2002-12-18 00:00:00

abstract：:Most tumor cells attain their immortality by reactivating telomerase. We report here the telomerase inhibitory potential of chimeric oligonucleotides composed of a 13mer antisense sequence targeting the telomerase RNA template region and a (s4dU)n moiety at its 3' or 5'-end. The increase of the thiolated chain length ...

journal_title：FEBS letters

authors： Tarkanyi I,Horváth A,Szatmari I,Eizert H,Vámosi G,Damjanovich S,Ségal-Bendirdjian E,Aradi J

更新日期：2005-02-28 00:00:00

abstract：:Constitutively secreted proteins have traditionally been believed to be excluded from the regulated secretory pathway. In this work we show that kappa light chain and Fc fragment, two markers of the constitutive pathway, are present in the regulated pathway in AtT-20 cells. They colocalize with the endogenous hormone ...

journal_title：FEBS letters

authors： Castle AM,Huang AY,Castle JD

更新日期：1998-11-20 00:00:00

abstract：:CAP (c-Cbl associated protein)/ponsin belongs to a family of adaptor proteins implicated in cell adhesion and signaling. Here we show that CAP binds to and co-localizes with the essential endocytic factor dynamin. We demonstrate that CAP promotes the formation of dynamin-decorated tubule like structures, which are als...

journal_title：FEBS letters

authors： Tosoni D,Cestra G

更新日期：2009-01-22 00:00:00

abstract：:In thrombin-stimulated platelets alpha IIb beta 3 integrin engagement triggers both phosphatidylinositol 3',4'-bisphosphate synthesis and calpain activation. We checked the possible involvement of calpains in phosphatidylinositol 3-kinase signalling pathway using a cell permeant specific inhibitor of calpains, calpept...

journal_title：FEBS letters

authors： Montsarrat N,Racaud-Sultan C,Mauco G,Plantavid M,Payrastre B,Breton-Douillon M,Chap H

更新日期：1997-03-03 00:00:00

abstract：:The pathogenicities of influenza viruses and paramyxoviruses have been proposed to be primarily determined by a host cell protease(s) that activates viral infectivity by proteolytic cleavage of the envelope glycoproteins. We recently isolated a trypsin-type endoprotease, named tryptase Clara, from rat bronchial and br...

journal_title：FEBS letters

authors： Kido H,Sakai K,Kishino Y,Tashiro M

更新日期：1993-05-10 00:00:00

abstract：:The chloroplast inner envelope translocon subunit Tic110 is imported via a soluble stromal translocation intermediate. In this study an in-organellar import system is established which allows for an accumulation of this intermediate in order to analyze its requirements for reexport. All results demonstrate that the re...

journal_title：FEBS letters

authors： Vojta L,Soll J,Bölter B

更新日期：2007-06-12 00:00:00

abstract：:We investigated here whether adiponectin can exhibit an inhibitory effect on tumor necrosis factor-alpha (TNF-alpha)- and receptor activator of nuclear factor-kappaB ligand (RANKL)-induced osteoclastogenesis by using RAW264 cell D clone with a high efficiency to form osteoclasts. Globular adiponectin (gAd) strongly in...

journal_title：FEBS letters

authors： Yamaguchi N,Kukita T,Li YJ,Kamio N,Fukumoto S,Nonaka K,Ninomiya Y,Hanazawa S,Yamashita Y

更新日期：2008-02-06 00:00:00

abstract：:Human kallikrein 8 (KLK8) is a member of the human kallikrein gene family of serine proteases, and its protein, hK8, has recently been suggested to serve as a new ovarian cancer marker. To gain insights into the physiological role of hK8, the active recombinant enzyme was obtained in a pure state for biochemical and e...

journal_title：FEBS letters

authors： Rajapakse S,Ogiwara K,Takano N,Moriyama A,Takahashi T

更新日期：2005-12-19 00:00:00

abstract：:Nuclear proteins are actively and posttranslationally transported across the nuclear envelope. This transport is a highly selective process that can be divided into two steps, receptor-binding followed by translocation through the nuclear envelope. Receptor-binding is mediated by nuclear localization signals that have...

journal_title：FEBS letters

authors： Wagner P,Kunz J,Koller A,Hall MN

更新日期：1990-11-26 00:00:00

abstract：:We previously isolated, by differential display and 5' RACE (rapid amplification of cDNA ends), cDNAs corresponding to genes activated following cryptogein treatment of tobacco cell suspensions, among them tcI 7 (tcI for tobacco cryptogein Induced), a gene encoding a beta-subunit of proteasome. Here, we report that tc...

journal_title：FEBS letters

authors： Etienne P,Petitot AS,Houot V,Blein JP,Suty L

更新日期：2000-01-28 00:00:00

abstract：:Targeting gene therapy vectors to abundant receptors on airway epithelia may allow a significant enhancement of gene delivery and thereby be of particular importance for the gene therapy of cystic fibrosis. Alpha9beta1-integrins are highly expressed throughout the human airway epithelia in vivo, irrespective of any pa...

journal_title：FEBS letters

authors： Schneider H,Harbottle RP,Yokosaki Y,Kunde J,Sheppard D,Coutelle C

更新日期：1998-06-16 00:00:00

abstract：:Acetaldehyde (1-20 mM) was metabolized at high rates and in a dose-dependent manner in isolated human and baboon kidney-cortex tubules. Acetaldehyde removal was accompanied by a large accumulation of acetate in both human and baboon tubules. By contrast, a large synthesis of ethanol was observed only in baboon tubules...

journal_title：FEBS letters

authors： Michoudet C,Baverel G

更新日期：1987-05-25 00:00:00

abstract：:The expression of the cytochrome P4501A1 gene, CYP1A1, is induced by e.g. 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) mainly by transcriptional mechanisms. The inducers mediate their effect upon binding and activation of the aryl hydrocarbon receptor (AHR) transcription-factor complex. Utilizing chimeric CYP1A1/CAT con...

journal_title：FEBS letters

authors： Jørgensen EC,Autrup H

更新日期：1995-05-29 00:00:00