Abstract:
:Constitutively secreted proteins have traditionally been believed to be excluded from the regulated secretory pathway. In this work we show that kappa light chain and Fc fragment, two markers of the constitutive pathway, are present in the regulated pathway in AtT-20 cells. They colocalize with the endogenous hormone ACTH and they exhibit stimulus-dependent secretion. The Fc fragment, which undergoes intracellular transport at the same rate as the ACTH precursor POMC, enters the forming secretory granules, however, it is partially lost during granule maturation. These observations show that classic constitutive secretory markers are not excluded from the regulated secretory pathway and that efficient sorting for regulated secretion occurs above a background of proteins which enter the granules without sorting.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Castle AM,Huang AY,Castle JDdoi
10.1016/s0014-5793(98)01398-2subject
Has Abstractpub_date
1998-11-20 00:00:00pages
341-5issue
3eissn
0014-5793issn
1873-3468pii
S0014-5793(98)01398-2journal_volume
439pub_type
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