Abstract:
:The photophobic receptor (psRII) and its transducer pHtrII from Natronobacterium pharaonis were heterologously coexpressed in the phototaxis-deficient Halobacterium salinarium strain Pho81/w which lacks all four bacterial rhodopsins, i.e. the two ion pumps bacteriorhodopsin and halorhodopsin as well as the two sensory pigments SRI and SRII. This genetically transformed Pho81/w strain showed a photophobic response upon illumination with blue light. The action spectrum of the psRII/pHtrII mediated phototactic behavior was determined in the range of 420-600 nm. The shape of the action spectrum was similar to the absorption spectrum of psRII, clearly indicating that the psRII-specific photophobic response in Pho81/w was restored. These results suggest that the pharaonis photoreceptor-transducer complex (psRII/pHtrII) is functionally competent to substitute the corresponding salinarium receptor system. Although the two archaea are phylogenetically quite distant from each other the two signal transduction chains are homologous systems which can replace each other.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Lüttenberg B,Wolff EK,Engelhard Mdoi
10.1016/s0014-5793(98)00322-6subject
Has Abstractpub_date
1998-04-10 00:00:00pages
117-20issue
1eissn
0014-5793issn
1873-3468pii
S0014-5793(98)00322-6journal_volume
426pub_type
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