Abstract:
:STAT4 is a transcription factor activated in response to IL-12, and is involved in Th1 cell development. The molecular mechanisms controlling the transcription of the STAT4 gene are however, unclear. Sequence comparison of the 5' flanking regions of human, mouse and pufferfish (Fugu rubripes) Stat4 genes revealed a high frequency of Ikaros (Ik) binding elements in all three species. We then investigated the role of Ik binding elements in the human STAT4 promoter using Jurkat T cells. Transactivation, electrophoretic mobility shift assay and RNA interference-mediated gene knockdown experiments revealed that Ik is involved in the regulation of STAT4 in human T cells.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Yap WH,Yeoh E,Tay A,Brenner S,Venkatesh Bdoi
10.1016/j.febslet.2005.07.018keywords:
subject
Has Abstractpub_date
2005-08-15 00:00:00pages
4470-8issue
20eissn
0014-5793issn
1873-3468pii
S0014-5793(05)00860-4journal_volume
579pub_type
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