Abstract:
:Selenium depletion of H4 hepatoma cells reduced cytosolic glutathione peroxidase (cGSH-Px) mRNA abundance but had no effect on phospholipid hydroperoxide glutathione peroxidase (PHGSH-Px) mRNA abundance. Actinomycin D chase experiments showed that selenium depletion had no effect on the stability of PHGSH-Px mRNA but decreased the stability of cGSH-Px mRNA. In Se-replete cells puromycin decreased the stability of both cGSH-Px and PHGSH-Px mRNAs. The results suggest that when selenium supply is limiting PHGSH-Px mRNA translation is maintained more than that of cGSH-Px mRNA, and thus more cGSH-Px mRNA is released from polysomes and degraded.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Bermano G,Arthur JR,Hesketh JEdoi
10.1016/0014-5793(96)00493-0subject
Has Abstractpub_date
1996-06-03 00:00:00pages
157-60issue
2-3eissn
0014-5793issn
1873-3468pii
0014-5793(96)00493-0journal_volume
387pub_type
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