Transient expression of botulinum neurotoxin C1 light chain differentially inhibits calcium and glucose induced insulin secretion in clonal beta-cells.

abstract：:DNA complementary to the rat hypothalamic mRNA coding for the corticotropin-releasing factor precursor (prepro-CRF) has been cloned by screening a cDNA library with a human genomic DNA probe. Nucleotide sequence analysis of the cloned cDNA has revealed that rat prepro-CRF consists of 187 amino acid residues including ...

journal_title：FEBS letters

authors： Jingami H,Mizuno N,Takahashi H,Shibahara S,Furutani Y,Imura H,Numa S

更新日期：1985-10-21 00:00:00

abstract：:The enzyme glucose-6-phosphate dehydrogenase from Trypanosoma cruzi (TcG6PDH) catalyses the first step of the pentose phosphate pathway (PPP) and is considered a promising target for the discovery of a new drug against Chagas diseases. In the present work, we describe the crystal structure of TcG6PDH obtained in a ter...

journal_title：FEBS letters

authors： Mercaldi GF,Dawson A,Hunter WN,Cordeiro AT

更新日期：2016-08-01 00:00:00

abstract：:We present evidence that gastrin, binding to a G protein-coupled receptor, activates the p38-mitogen-activated protein kinase (MAPK) pathway. Blockage of protein kinase C (PKC) by GF109203X, depletion of intracellular calcium by thapsigargin or inhibition of Src family kinases by PP2 prevented p38-MAPK activation and ...

journal_title：FEBS letters

authors： Dehez S,Daulhac L,Kowalski-Chauvel A,Fourmy D,Pradayrol L,Seva C

更新日期：2001-05-04 00:00:00

abstract：:Adenosine and ATP modulate cellular and tissue functions via specific P1 and P2 receptors, respectively. Although, in general, adenosine inhibits excitability and ATP functions as an excitatory transmitter in the central nervous system, little is known about the direct interaction between P1 and P2 receptors. We recen...

journal_title：FEBS letters

authors： Yoshioka K,Hosoda R,Kuroda Y,Nakata H

更新日期：2002-11-06 00:00:00

abstract：:It has been suggested that alpha-dystroglycan links the dystrophin-associated protein complex and extracellular matrix and that the absence of dystrophin and alpha-dystroglycan in Duchenne muscular dystrophy (DMD) may lead to the breakdown of this linkage. In the present study, myotubes from DMD patients and murine X-...

journal_title：FEBS letters

authors： Angoli D,Corona P,Baresi R,Mora M,Wanke E

更新日期：1997-05-26 00:00:00

abstract：:Recombinant porcine growth hormone (rPGH) was solubilised from inclusion bodies (IB's) using either 6 M guanidinium hydrochloride (GnHCl), 7.5 M urea or by a novel method using a cationic surfactant, cetyltrimethylammonium chloride (CTAC). Circular dichroism (CD) analysis of the secondary (2 degrees) structure of the ...

journal_title：FEBS letters

authors： Puri NK,Cardamone M

更新日期：1992-07-06 00:00:00

abstract：:In response to growth factors, quiescent fibroblast mutants lacking Na+/H+ exchange activity fail to elevate their cytoplasmic pH (pHi) and to reinitiate DNA synthesis at neutral and acidic pHo. A pHi threshold of approximately 7.2 exists, below which growth factors cannot set in motion the Go to S phase transition. R...

journal_title：FEBS letters

authors： Pouysségur J,Franchi A,L'Allemain G,Paris S

更新日期：1985-10-07 00:00:00

abstract：:Analysis of fluorimetric equilibrium-binding isotherms of a proteolytic fragment of bacteriophage T4 gene 32 protein (g32P) lacking residues 1-9 shows that this region contains the site responsible for the function of the NH2-terminal 'B' domain (residues 1-21). The end codon of the frameshift mutant g32P-PR201 has be...

journal_title：FEBS letters

authors： Casas-Finet JR

更新日期：1989-06-05 00:00:00

abstract：:We have identified and characterized a genomic DNA fragment containing the coding sequences corresponding to the human gamma-glutamyltransferase type 1 mRNA. The coding part of the gene spans over 16 kb and comprises 12 exons and 11 introns exhibiting a similar organization as for the mouse and rat GGT genes. The exon...

journal_title：FEBS letters

authors： Leh H,Chikhi N,Ichino K,Guellaën G,Wellman M,Siest G,Visvikis A

更新日期：1998-08-28 00:00:00

abstract：:The distribution of phosphatidylcholine (PC) and sphingomyelin (SM) between the solubilized (micellar) and non-solubilized (lamellar) fractions arising from bilayers composed of PC and SM, with or without cholesterol (Chol) has been measured under conditions of partial, incomplete solubilization by Triton X-100. Quant...

journal_title：FEBS letters

authors： Ahyayauch H,Collado MI,Goñi FM,Lichtenberg D

更新日期：2009-09-03 00:00:00

abstract：:The early response gene IEX-1 modulates apoptosis and cell growth in a poorly defined fashion. Here, we describe the effect of hammerhead ribozymes specifically disrupting IEX-1 expression in 293 cells. Compared to vector control, 293 cells exhibit a reduced growth rate and a slowed cell cycle progression, when stably...

journal_title：FEBS letters

authors： Grobe O,Arlt A,Ungefroren H,Krupp G,Fölsch UR,Schmidt WE,Schäfer H

更新日期：2001-04-13 00:00:00

abstract：:It was of interest to examine whether yeast Cu-thionein could be used to transfer the thiolate bound copper directly into the copper binding site of 'blue' apoproteins which contain free thiol groups. In particular apo-stellacyanin was used in the present study and it was found to be able to accept Cu(I) from yeast Cu...

journal_title：FEBS letters

authors： Hartmann HJ,Morpurgo L,Desideri A,Rotilio G,Weser U

更新日期：1983-02-07 00:00:00

abstract：:A human teratoma cell line (Tera 2) was grown in serum-free medium, and the population multiplication was stimulated by the addition of 1-10 ng basic fibroblast growth factor (bFGF)/ml. The bFGF-effect was abrogated by the addition of protamine sulphate. When high concentrations of bFGF were added, a preferential effe...

journal_title：FEBS letters

authors： Schofield PN,Granerus M,Lee A,Ektröm TJ,Engström W

更新日期：1992-02-24 00:00:00

abstract：:Calmodulin is shown to interact with human spectrin dimer. The binding was highly calcium-dependent and observed in two different kinds of experiments. Firstly, affinity chromatography of calmodulin on a Sepharose 4B column with immobilized spectrin, and secondly, partition in aqueous two-phase polymer systems. In the...

journal_title：FEBS letters

authors： Berglund A,Backman L,Shanbhag VP

更新日期：1984-06-25 00:00:00

abstract：:The half-lives of mRNAs in yeast vary from about 1 to over 100 min. While mRNA stabilities must strongly influence overall gene expression in this organism, very little is known about how they are determined. Labellings of yeast cells were conducted to investigate whether the 5'-cap structures of yeast mRNAs might inf...

journal_title：FEBS letters

authors： Piper PW,Curran B,Davies W,Hirst K,Seward K

更新日期：1987-08-10 00:00:00

abstract：:We report a new member of the von Willebrand factor A-domain protein superfamily, WARP (for von Willebrand factor A-domain-related protein). The full-length mouse WARP cDNA is 2.3 kb in size and predicts a protein of 415 amino acids which contains a signal sequence, a VA-like domain, two fibronectin type III-like repe...

journal_title：FEBS letters

authors： Fitzgerald J,Tay Ting S,Bateman JF

更新日期：2002-04-24 00:00:00

abstract：:An electrometric technique was used to investigate electron transfer between spinach plastocyanin (Pc) and photooxidized primary electron donor P700 in photosystem I (PS I) complexes from the cyanobacterium Synechocystis sp. PCC 6803. In the presence of Pc, the fast unresolvable kinetic phase of membrane potential gen...

journal_title：FEBS letters

authors： Mamedov MD,Mamedova AA,Chamorovsky SK,Semenov AY

更新日期：2001-07-06 00:00:00

abstract：:The cytochrome bc1 complex is a key component of the mitochondrial respiratory chains of many eukaryotic microorganisms that are pathogenic for plants or humans, such as fungi responsible for crop diseases and Plasmodium falciparum, which causes human malaria. Cytochrome bc1 is an enzyme that contains two (ubi)quinone...

journal_title：FEBS letters

authors： Fisher N,Meunier B,Biagini GA

更新日期：2020-09-01 00:00:00

abstract：:Aah VI was isolated from the venom of the North African scorpion, Androctonus australis hector. It is the first glycosylated neurotoxin from scorpion venom to be described. It was not toxic to mice, when injected intracerebroventricularly at a dose of 1.2 microg per animal. However, it had typical activity in Blatella...

journal_title：FEBS letters

authors： Hassani O,Loew D,Van Dorsselaer A,Papandréou MJ,Sorokine O,Rochat H,Sampieri F,Mansuelle P

更新日期：1999-01-25 00:00:00

abstract：:The intestinal microbiota is essential for nutrient acquisition, immune development, and exclusion of invading pathogens. The upper respiratory tract (URT) microbiota is less well studied and does not appear to abide by many of the paradigms of the gastrointestinal tract. Decades of carriage studies in children have d...

journal_title：FEBS letters

authors： Schenck LP,Surette MG,Bowdish DM

更新日期：2016-11-01 00:00:00

abstract：:We report a nonenzymatic reaction which results in the radiolabeling of proteins by 32P-containing nucleoside triphosphates. The labeling reaction does not require any cofactors, but is greatly enhanced by the presence of alcohols. Even under optimal conditions, less than 1% of the protein molecules undergo modificati...

journal_title：FEBS letters

authors： Schmidt MC,Hanna MM

更新日期：1986-01-06 00:00:00

abstract：:Phosphorylation of H2AX is believed to be associated with the repair of damaged DNA. Recent findings suggest a novel function of H2AX in cellular apoptosis. Specifically, it was shown that ultraviolet A-activated JNK phosphorylates H2AX to regulate apoptosis. Here we show that serum starvation induces H2AX phosphoryla...

journal_title：FEBS letters

authors： Lu C,Shi Y,Wang Z,Song Z,Zhu M,Cai Q,Chen T

更新日期：2008-08-06 00:00:00

abstract：:HSP73, the constitutive form of heat-shock protein 70, has been implicated in the translocation of preproteins across the mitochondrial membranes, being required for maintaining mitochondrial preproteins in an import competent conformation. Here we report that highly purified mitochondrial outer membranes contain a pr...

journal_title：FEBS letters

authors： Lithgow T,Ryan M,Anderson RL,Høj PB,Hoogenraad NJ

更新日期：1993-10-18 00:00:00

abstract：:The membrane-bound 3-chloro-4-hydroxyphenylacetate (Cl-OHPA) reductive dehalogenase from the chlorophenol-reducing anaerobe Desulfitobacterium hafniense was purified 11.3-fold to apparent homogeneity in the presence of the detergent CHAPS. The purified dehalogenase catalyzed the reductive dechlorination of Cl-OHPA to ...

journal_title：FEBS letters

authors： Christiansen N,Ahring BK,Wohlfarth G,Diekert G

更新日期：1998-10-02 00:00:00

abstract：:Glucose-fructose oxidoreductase (GFOR), a periplasmic protein of Zymomonas mobilis, is synthesized as a precursor polypeptide with a twin-R signal peptide for Sec-independent protein export in bacteria. In higher plant chloroplasts, twin-R signal peptides are specific targeting signals for the Sec-independent delta pH...

journal_title：FEBS letters

authors： Halbig D,Hou B,Freudl R,Sprenger GA,Klösgen RB

更新日期：1999-03-19 00:00:00

abstract：:The relationship among the real-time trafficking of lung metastatic B16BL6 cells, metastatic potential, and the injected number of the cells was examined, since the smaller the number of tumor cells injected, the more clearly the immune defense may be observed. When 1x10(6) or 1x10(5) B16BL6 cells were injected into m...

journal_title：FEBS letters

authors： Kikkawa H,Imafuku H,Tsukada H,Oku N

更新日期：2000-02-11 00:00:00

abstract：:The mechanism of store-operated Ca2+ inflow in hepatocytes was investigated using fluo-3 and fura-2 to monitor changes in the concentration of intracellular free Ca2+ in single cells, and 1-(alpha-glycerophosphoryl)-myo-inositol 4,5-diphosphate, P4(5)-1-(2-nitrophenyl)ethyl ester ('caged' GPIP2) and 'caged' guanosine ...

journal_title：FEBS letters

authors： Berven LA,Barritt GJ

更新日期：1994-06-13 00:00:00

abstract：:To investigate the importance of the seventh residue of the second and third repeat fragments (R2 and R3 peptides) of the microtubule-binding domain (MBD) for tau filamentous assembly, the residues Lys and Pro were substituted (R2-K7P and R3-P7K). The filament formations of the R2 and R3 peptides were almost lost due ...

journal_title：FEBS letters

authors： Tokimasa M,Minoura K,Hiraoka S,Tomoo K,Sumida M,Taniguchi T,Ishida T

更新日期：2005-07-04 00:00:00

abstract：:The -proteobacterium Wolinella succinogenes grows anaerobically by respiratory nitrite ammonification but not by denitrification. Nevertheless, it is capable of N(2)O reduction to N(2). Recently, the genome sequence of W. succinogenes revealed a nos gene cluster with intriguing features encoding a new type of N(2)O re...

journal_title：FEBS letters

authors： Simon J,Einsle O,Kroneck PM,Zumft WG

更新日期：2004-07-02 00:00:00

abstract：:We present a rat model in which continuous supply of hepatocyte growth factor (HGF) prevents liver injury induced by carbon tetrachloride (CCl4) and E. coli 011:B4 lipopolysaccharide (LPS). Rat fibroblasts genetically modified to secrete rat HGF were implanted in syngenic rat spleen 7 days before administration of the...

journal_title：FEBS letters

authors： Kaido T,Yamaoka S,Seto S,Funaki N,Kasamatsu T,Tanaka J,Nakamura T,Imamura M

更新日期：1997-07-14 00:00:00