Abstract:
:Aah VI was isolated from the venom of the North African scorpion, Androctonus australis hector. It is the first glycosylated neurotoxin from scorpion venom to be described. It was not toxic to mice, when injected intracerebroventricularly at a dose of 1.2 microg per animal. However, it had typical activity in Blatella germanica cockroaches resulting in gradual paralysis and very low toxicity (LD50 = 8.5 microg/g of animal). It consists of 66 amino acid residues and is heterogeneously N-glycosylated at a single site, on asparagine 9, of the Asn-Gly-Thr sequence. The potential N-glycosylation site was deduced from automatic Edman degradation and amino acid analysis, and glycan heterogeneity was evidenced by ESMS. Determination of the N-glycan structures (dHex, Hex and HexNAc) was assessed by nanoESMS/MS with picomolar amounts of sample. Current knowledge of N-glycan structure and composition suggests that the glycan structures are derived from a common core.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Hassani O,Loew D,Van Dorsselaer A,Papandréou MJ,Sorokine O,Rochat H,Sampieri F,Mansuelle Pdoi
10.1016/s0014-5793(98)01710-4subject
Has Abstractpub_date
1999-01-25 00:00:00pages
175-80issue
2eissn
0014-5793issn
1873-3468pii
S0014-5793(98)01710-4journal_volume
443pub_type
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