Abstract:
:Steady-state kinetic analysis was performed on the reaction between D-fructose and ferricyanide with the quinohemoprotein fructose dehydrogenase from Gluconobacter species. The D-fructose oxidation dependence on the ferricyanide concentration resulted in a series of parallel reciprocal plots, and the reaction was assumed to proceed by a ping-pong type of mechanism. A reciprocal plot of the reduction of ferricyanide at saturating concentration of D-fructose gave a break which was considered to appear as a result of the two active centers, namely PQQ and heme c functioning. A scheme of action is proposed and the bimolecular rate constant of the D-fructose oxidation, the kcat for PQQ and the electron transfer rate between the PQQH2 and heme c are calculated and account for 2.2 +/- 0.4 x 10(4) M-1 s-1, (93 +/- 14) and (162 +/- 7) s-1, respectively.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Marcinkeviciene J,Johansson Gdoi
10.1016/0014-5793(93)81319-usubject
Has Abstractpub_date
1993-02-22 00:00:00pages
23-6issue
1eissn
0014-5793issn
1873-3468pii
0014-5793(93)81319-Ujournal_volume
318pub_type
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