Abstract:
:Ribosome display is a powerful tool for selecting and evolving protein functions through ligand-binding. Here, this in vitro system was used to perform selection based on the folding properties of proteins, independent of specific ligand-binding. The selection is based on two properties of misfolded proteins: (1) increased sensitivity to proteolysis and (2) greater exposure of hydrophobic area. By targeting these properties, we show that compactly folded and soluble proteins can be enriched over insoluble and random coil proteins. This approach may be especially useful for selection and evolution of folded proteins from random sequence libraries.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Matsuura T,Plückthun Adoi
10.1016/s0014-5793(03)00178-9keywords:
subject
Has Abstractpub_date
2003-03-27 00:00:00pages
24-8issue
1-3eissn
0014-5793issn
1873-3468pii
S0014579303001789journal_volume
539pub_type
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