Abstract:
:The exposure of cells for 60 min to a serum free medium at ice temperature followed by a return to normal culture conditions (30 min at 37 degrees C) caused a dramatic decrease in NAD+ levels. This decrease in NAD+ was prevented by 3-aminobenzamide. Alkaline elution analysis of DNA from cultures that were sisters to the ones utilized for measuring cellular NAD+ content revealed an absence of DNA breakage. These data suggest that poly(ADP-ribose)transferase may be induced in conditions not involving DNA fragmentation. The induction of this enzyme could therefore represent a cellular emergency reaction and not just a response to DNA damage.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Cantoni O,Sestili P,Cattabeni F,Stocchi Vdoi
10.1016/0014-5793(86)80825-0subject
Has Abstractpub_date
1986-08-18 00:00:00pages
266-8issue
2eissn
0014-5793issn
1873-3468pii
0014-5793(86)80825-0journal_volume
204pub_type
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