FTIR spectroscopy shows weak symmetric hydrogen bonding of the QB carbonyl groups in Rhodobacter sphaeroides R26 reaction centres.

abstract：:Fibroblast growth factor-2 (FGF-2) is mitogenic for the human breast cancer cell line MCF-7; here we investigate some of the signaling pathways subserving this activity. FGF-2 stimulation of MCF-7 cells resulted in a global increase of intracellular tyrosine phosphorylation of proteins, particularly FGF receptor subst...

journal_title：FEBS letters

authors： Vercoutter-Edouart A,Lemoine J,Smart CE,Nurcombe V,Boilly B,Peyrat J,Hondermarck H

更新日期：2000-08-04 00:00:00

abstract：:Homologues of the catalase-peroxidase gene katG and the gene for the non-specific DNA binding protein dpsA were identified downstream of oxyR in Burkholderia pseudomallei. Northern experiments revealed that both katG and dpsA are co-transcribed during oxidative stress. Under conditions where the katG promoter is not h...

journal_title：FEBS letters

authors： Loprasert S,Whangsuk W,Sallabhan R,Mongkolsuk S

更新日期：2003-05-08 00:00:00

abstract：:Rabbit red blood cells of various ages were separated on Percoll gradients and the activities of two large cytosolic proteases were measured. Both the multicatalytic protease (MCP), assayed by hydrolysis of fluorigenic peptides, and the 26 S ubiquitin/ATP-stimulated protease, assayed by degradation of ubiquitin-lysozy...

journal_title：FEBS letters

authors： Di Cola D,Pratt G,Rechsteiner M

更新日期：1991-03-11 00:00:00

abstract：:The toxicity of the beta-amyloid (Abeta) peptide of Alzheimer's disease may relate to its polymerisation state (i.e. fibril content). We have shown previously that plasma lipoproteins, particularly when oxidised, greatly enhance Abeta polymerisation. In the present study the nature of the interactions between both nat...

journal_title：FEBS letters

authors： Stanyer L,Betteridge DJ,Smith CC

更新日期：2002-05-08 00:00:00

abstract：:Ca2+ release from sarcoplasmic reticulum membranes, activated by alkaline pH occurs only when EGTA is present in the release medium. Addition of very low concentrations of Ca2+ to the medium inhibits Ca2+ release. The concentration of free Ca2+ required for 50% inhibition ranges from between 5 and 20 nM in different e...

journal_title：FEBS letters

authors： Argaman A,Shoshan-Barmatz V

更新日期：1989-01-16 00:00:00

abstract：:Hemoglobin-binding proteins are necessary for pathogens to obtain iron from Hb. Entamoeba histolytica can grow using Hb as source of iron, but the underlying mechanism has not previously been established. In this work, we identified a 45 kDa Hb-binding protein of E. histolytica, which we named Ehhmbp45. In silico anal...

journal_title：FEBS letters

authors： Cruz-Castañeda A,Olivares-Trejo JJ

更新日期：2008-08-06 00:00:00

abstract：:Alix and its homologs are involved in various phenomena such as endosomal protein-sorting and adaptation to stress conditions. In this study, we found that development of Dictyostelium discoideum Alix (DdAlix) deletion mutant (alx-) cells was impaired in alkaline pH environments. The fruiting body formation efficiency...

journal_title：FEBS letters

authors： Ohkouchi S,Saito H,Aruga F,Maeda T,Shibata H,Maki M

更新日期：2005-03-14 00:00:00

abstract：:The high cGMP sensitivity of cAMP-dependent protein kinase A (type II) (PKAII) from invertebrates led to the hypothesis that cGMP directly activates PKAII under physiological conditions. We tested this idea using PKAII holoenzyme purified from the honeybee brain in an assay with short stimulation times. In the presenc...

journal_title：FEBS letters

authors： Leboulle G,Müller U

更新日期：2004-10-08 00:00:00

abstract：:The red cell membrane is comprised of a lipid bilayer studded with transmembrane proteins, and laminated by a protein network, the membrane skeleton, at the surface of the inner monolayer. The erythrocyte owes its mechanical properties to the membrane skeleton. Hereditary spherocytosis, hereditary elliptocytosis or po...

journal_title：FEBS letters

authors： Delaunay J

更新日期：1995-08-01 00:00:00

abstract：:Tyrosine O-sulfation is one of the post-translational modification processes that occur to membrane proteins and secreted proteins in eukaryotes. Tyrosylprotein sulfotransferase (TPST) is responsible for this modification, and in this report, we describe the expression pattern and the biological role of TPST-A in the ...

journal_title：FEBS letters

authors： Kim TH,Hwang SB,Jeong PY,Lee J,Cho JW

更新日期：2005-01-03 00:00:00

abstract：:3C-like (3CL) protease is essential for the life cycle of severe acute respiratory syndrome-coronavirus (SARS-CoV) and therefore represents a key anti-viral target. A compound library consisting of 960 commercially available drugs and biologically active substances was screened for inhibition of SARS-CoV 3CL protease....

journal_title：FEBS letters

authors： Hsu JT,Kuo CJ,Hsieh HP,Wang YC,Huang KK,Lin CP,Huang PF,Chen X,Liang PH

更新日期：2004-09-10 00:00:00

abstract：:The effect of angiotensin II (Ang II) on the early growth response gene-1 (Egr-1) mRNA, on the Egr-1 protein and on the phosphoinositide PI turnover signalling system was investigated in the presence and absence of EXP3174, a potent non-peptide Ang II receptor antagonist. Ang II induced an accumulation of 3.4 kb Egr-1...

journal_title：FEBS letters

authors： Sachinidis A,Weisser P,Ko Y,Schulte K,Meyer zu Brickwedde K,Neyses L,Vetter H

更新日期：1992-11-23 00:00:00

abstract：:Guanylate cyclase activity was measured in disrupted rod outer segments of the toad retina. The experiments showed that cGMP is synthesized from GTP at a rate of 3 +/- 1 nmol/min per mg protein. In darkness this value is largely independent of the Ca2+ concentration, while it is enhanced by flashes of light of increas...

journal_title：FEBS letters

authors： Pepe IM,Panfoli I,Cugnoli C

更新日期：1986-07-14 00:00:00

abstract：:Genetic factors seem to play a significant role in susceptibility to systemic lupus erythematosus (SLE). We previously described the amino acid polymorphism (Val14Met) within the IFN-gamma receptor 1 (IFN-gammaRI), and that the frequency of the Metl4 allele in SLE patients was significantly higher than that of the hea...

journal_title：FEBS letters

authors： Nakashima H,Inoue H,Akahoshi M,Tanaka Y,Yamaoka K,Ogami E,Nagano S,Arinobu Y,Niiro H,Otsuka T,Niho Y

更新日期：1999-06-18 00:00:00

abstract：:Elongation factor Tu (EF-Tu) is the most abundant protein in prokaryotic cells. Its general function in protein biosynthesis is well established. It is a member of the large family of G-proteins, all of which bind guanosine phosphates (GDP or GTP) as cofactors. In its active GTP bound state EF-Tu binds aminoacylated t...

journal_title：FEBS letters

authors： Nissen P,Reshetnikova L,Siboska G,Polekhina G,Thirup S,Kjeldgaard M,Clark BF,Nyborg J

更新日期：1994-12-19 00:00:00

abstract：:Using a prostate-specific antigen cDNA as a hybridization probe, clones containing the kallikrein genes encoding prostate-specific antigen, human glandular kallikrein-1 and pancreas/kidney kallikrein were isolated from a human genomic library. Clones containing the prostate-specific antigen gene and the human glandula...

journal_title：FEBS letters

authors： Riegman PH,Vlietstra RJ,Klaassen P,van der Korput JA,Geurts van Kessel A,Romijn JC,Trapman J

更新日期：1989-04-10 00:00:00

abstract：:We used cDNA microarray to identify transforming growth factor beta (TGF-beta) responsive target genes during osteoblast development and found that nephronectin (Npnt) is one such gene that is significantly down-regulated. Here we report the role of TGF-beta in regulating Npnt-mediated osteoblast differentiation. We f...

journal_title：FEBS letters

authors： Fang L,Kahai S,Yang W,He C,Seth A,Peng C,Yang BB

更新日期：2010-07-02 00:00:00

abstract：:Iron regulatory protein 2 (IRP2) is a critical switch for cellular and systemic iron homeostasis. In iron-deficient or hypoxic cells, IRP2 binds to mRNAs containing iron responsive elements (IREs) and regulates their expression. Iron promotes proteasomal degradation of IRP2 via the F-box protein FBXL5. Here, we explor...

journal_title：FEBS letters

authors： Hausmann A,Lee J,Pantopoulos K

更新日期：2011-02-18 00:00:00

abstract：:An Arabidopsis cDNA coding for a previously uncharacterized isoform of inorganic pyrophosphatase was isolated. It was used to complement an E. coli mutant, demonstrating that it coded for an active enzyme. MgCl(2) was necessary for the protein's activity, whilst NaF inhibited it. The K(m) for pyrophosphate and the pH ...

journal_title：FEBS letters

authors： Schulze S,Mant A,Kossmann J,Lloyd JR

更新日期：2004-05-07 00:00:00

abstract：:The peptidic siderophore pyoverdine is the primary iron uptake system of fluorescent pseudomonads, and a virulence factor in the opportunistic pathogen Pseudomonas aeruginosa. Pyoverdine biogenesis is a co-ordinate process requiring several precursor-generating enzymes and large nonribosomal peptide synthetases (NRPSs...

journal_title：FEBS letters

authors： Imperi F,Visca P

更新日期：2013-11-01 00:00:00

abstract：:RKIP was first identified as an inhibitor of the Raf-MEK-ERK signaling pathway. RKIP was also found to play an important role in the NF-kappaB pathway. Genetic and biochemical studies demonstrated that RKIP functioned as a scaffold protein facilitating the phosphorylation of IkappaB by upstream kinases. However, contr...

journal_title：FEBS letters

authors： Tang H,Park S,Sun SC,Trumbly R,Ren G,Tsung E,Yeung KC

更新日期：2010-02-19 00:00:00

abstract：:The mediator complex in the RNA polymerase II holoenzyme is known to be involved in transcriptional activation. The role of the essential mediator component Srb7p has been difficult to investigate, since no conditional lethal allele has been available to date. While the expression of Srb7p under the control of a repre...

journal_title：FEBS letters

authors： Gromöller A,Lehming N

更新日期：2000-10-27 00:00:00

abstract：:Lipid metabolism is extensively studied in Saccharomyces cerevisiae. Here, we report that revertant of glycogen synthase kinase mutation-1 (Rog1p) possesses monoacylglycerol (MAG) lipase activity in S. cerevisiae. The lipase activity of Rog1p was confirmed in two ways: through analysis of a strain with a double deleti...

journal_title：FEBS letters

authors： Vishnu Varthini L,Selvaraju K,Srinivasan M,Nachiappan V

更新日期：2015-01-02 00:00:00

abstract：:Genetically obese (ob/ob) mice develop a marked insulin resistance in brown adipose tissue soon after weaning, and this is paralleled by a fall in the acute activation of the mitochondrial proton conductance pathway in the tissue on cold exposure. Treatment of ob/ob mice with ciglitazone, a new oral hypoglycaemic, led...

journal_title：FEBS letters

authors： Mercer SW,Trayhurn P

更新日期：1986-01-20 00:00:00

abstract：:The large rep gene products, Rep78 and Rep68, of adeno-associated virus (AAV) are pleiotropic effector proteins which are required for AAV DNA replication and the trans-regulation of AAV gene expression. Apart from these essential functions prerequisite for the life cycle of AAV, these rep products are able to inhibit...

journal_title：FEBS letters

authors： Batchu RB,Hermonat PL

更新日期：1995-07-03 00:00:00

abstract：:Abstract Type V adenylyl cyclase (AC) was stably over-expressed in HEK293 cells (293AC-V). Forskolin-stimulated cAMP accumulation in 293AC-V was 5 times as great as that in control cells. PMA, a protein kinase C (PKC) activator, enhanced cAMP accumulation in 293AC-V cells dose-and time-dependently and this enhancement...

journal_title：FEBS letters

authors： Kawabe Ji,Ebina T,Toya Y,Oka N,Schwencke C,Duzic E,Ishikawa Y

更新日期：1996-04-22 00:00:00

abstract：:We have used ultraviolet laser crosslinking to characterize the DNA-binding properties of highly purified yeast topoisomerase II in the absence of ATP. A single 5 ns, 20 mJ pulse of 266 nm light produced optimal crosslinking to a short DNA duplex, with an efficiency of 0.25%. An equilibrium binding constant (Keq) of 1...

journal_title：FEBS letters

authors： Hung F,Luo D,Sauvé DM,Muller MT,Roberge M

更新日期：1996-02-12 00:00:00

abstract：:The purpose of this investigation was to study the effect on cap structure for trans-splicing in mammalian cells. The early SV40 Bst/Bam pre-mRNA (cRNA) was synthesized in vitro in both capped (cap-Bst/Bam-cRNA) and non-capped (Bst/Bam-cRNA) versions and microinjected into the nuclei of TC7 cells. Trans-splicing was m...

journal_title：FEBS letters

authors： Graessmann M,Eul J,Berg B,Zimmermann C,Graessmann A

更新日期：1996-09-30 00:00:00

abstract：:T helper 1 (Th1) cell fate is induced by overlapping signaling pathways, whose kinetic principles and regulatory motifs are largely unknown. We identified a simple positive feedback loop in the STAT4 signaling pathway, whereby activation by IL-12 leads to the increased expression in IL-12 receptor. A computational ana...

journal_title：FEBS letters

authors： Becskei A,Grusby MJ

更新日期：2007-11-13 00:00:00

abstract：:We describe the extraction and stabilization in vitro of discrete complexes of interferon and cellular receptor proteins. A homogeneous complex of Mr 230 000 was extracted at the time of peak receptor binding (30 min). Complex formation was specific for human interferon. At later times a second complex could also be e...

journal_title：FEBS letters

authors： Eid P,Mogensen KE

更新日期：1983-05-30 00:00:00