Abstract:
:The purpose of this investigation was to study the effect on cap structure for trans-splicing in mammalian cells. The early SV40 Bst/Bam pre-mRNA (cRNA) was synthesized in vitro in both capped (cap-Bst/Bam-cRNA) and non-capped (Bst/Bam-cRNA) versions and microinjected into the nuclei of TC7 cells. Trans-splicing was monitored by immunofluorescence staining (T1-antigen) and by RT-PCR analysis. Cap-Bst/Bam-cRNA was trans-spliced with high efficiency, but not the Bst/Bam-cRNA molecules. Northern blot analysis revealed that both the capped and uncapped cRNA molecules had similar stability in the microinjected cells. The coinjected m7G(5')ppp(5')G cap analog did not inhibit the trans-splicing reaction in vivo and did not prevent nuclear export of the mRNA.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Graessmann M,Eul J,Berg B,Zimmermann C,Graessmann Adoi
10.1016/0014-5793(96)00956-8subject
Has Abstractpub_date
1996-09-30 00:00:00pages
233-6issue
2eissn
0014-5793issn
1873-3468pii
0014-5793(96)00956-8journal_volume
394pub_type
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