Abstract:
:Increases in choline containing metabolites have been associated with a number of disorders, including malignant cell growth. In this study, high resolution magic angle spinning (1)H nuclear magnetic resonance spectroscopy was employed to monitor metabolite changes during cell transfection, and an increase in phosphocholine was detected. This increase appears to be correlated with cell membrane disruption associated with the insertion of plasmid DNA into cells, since the level of phosphocholine in mock transfected cells was comparable to that of control cells. These data suggest choline containing metabolite changes detected in vivo using magnetic resonance spectroscopy relate to cell membrane disruption.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Griffin JL,Mann CJ,Scott J,Shoulders CC,Nicholson JKdoi
10.1016/s0014-5793(01)03175-1keywords:
subject
Has Abstractpub_date
2001-12-07 00:00:00pages
263-6issue
2eissn
0014-5793issn
1873-3468pii
S0014-5793(01)03175-1journal_volume
509pub_type
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