Label-free optical biosensor with microfluidics for sensing ligand-directed functional selectivity on trafficking of thrombin receptor.

abstract：:Mitogen activated protein kinase in extracts of U-937 macrophage-like cells was stimulated by LDL and oxLDL. A maximum value (161% of the basal phosphotransferase activity) was obtained after 6 min exposure to oxidized LDL (27 microgram/ml) using APRTPGGRR peptide substrate. The activatory effect was more pronounced (...

journal_title：FEBS letters

authors： Deigner HP,Claus R

更新日期：1996-05-06 00:00:00

abstract：:Investigations of the cleavage specificity of a cyclizing endoribonuclease of yeast show that it hydrolyzes Y-A bonds of single-stranded regions of RNA and that oligonucleotides are hydrolyzed poorly. The stringent specificity of the enzyme suggests that it may be useful for generating specific fragments of polyribonu...

journal_title：FEBS letters

authors： Stevens A

更新日期：1986-09-15 00:00:00

abstract：:Immunoblotting analysis of purified human urokinase plasminogen activator (u-PA), gives a positive signal when reacted with anti-phosphotyrosine monoclonal antibodies (MoAb anti-P-Tyr); competition with o-phospho-DL-tyrosine (P-Tyr) but not o-phospho-DL-threonine or serine (P-Treo, P-Ser) completely suppresses this si...

journal_title：FEBS letters

authors： Barlati S,Paracini F,Bellotti D,De Petro G

更新日期：1991-04-09 00:00:00

abstract：:Specific activity was compared between wild-type (WT) neutral protease from Bacillus stearothermophilus and mutant protease (M1; Gly144 replaced by Ala144) with enhanced thermostability. When casein was used as a substrate, M1 showed 1.5-times higher specific activity than that of WT. In contrast, the specific activit...

journal_title：FEBS letters

authors： Takagi M,Imanaka T

更新日期：1989-08-28 00:00:00

abstract：:The carboxy-terminal half of the c-src protein fused to the protein A moiety was expressed in bacteria. The protein A/truncated c-src fusion protein, which does not have SH2 and SH3 domains, is found in the periplasmic space allowing for a simple one-step purification and demonstrated high efficiency in autophosphoryl...

journal_title：FEBS letters

authors： Saya H,Lee PS,Nishi T,Izawa I,Nakajima M,Gallick GE,Levin VA

更新日期：1993-07-26 00:00:00

abstract：:Pretreatment of tissues with potassium channel openers (KCO's) has been observed to be cytoprotective in a broad variety of insults. This phenomenon has been proposed to be intimately linked to activation of mitochondrial potassium channels which apparently modulate the mitochondrial production of reactive oxygen spec...

journal_title：FEBS letters

authors： Malinska D,Mirandola SR,Kunz WS

更新日期：2010-05-17 00:00:00

abstract：:We have previously generated transgenic (Tg) mice expressing the human apolipoprotein (apo) A-I/C-III/A-IV gene cluster. This expression induced hyperlipidemia but reduced atherosclerotic lesions in genetically modified mice lacking apoE. Atherosclerosis is a multifactorial process and environmental factors such as di...

journal_title：FEBS letters

authors： Baroukh N,Ostos MA,Vergnes L,Recalde D,Staels B,Fruchart J,Ochoa A,Castro G,Zakin MM

更新日期：2001-07-27 00:00:00

abstract：:Calcium release from internal stores of Entamoeba histolytica, a parasitic protozoan, was observed by measuring fluorescence of Fura-2. Emptying of inositol(1,4,5)trisphosphate (Ins(1,4,5)P3)-sensitive calcium pools in permeabilized E. histolytica did not significantly affect subsequent calcium release by inositol(1,3...

journal_title：FEBS letters

authors： Raha S,Giri B,Bhattacharyya B,Biswas BB

更新日期：1995-04-10 00:00:00

abstract：:Proteins implicated as intracellular chloride channels include the intracellular ClC proteins, the bestrophins, the cystic fibrosis transmembrane conductance regulator, the CLICs, and the recently described Golgi pH regulator. This paper examines current hypotheses regarding roles of intracellular chloride channels an...

journal_title：FEBS letters

authors： Edwards JC,Kahl CR

更新日期：2010-05-17 00:00:00

abstract：:miRNAs play a number of roles in bone, including mediating the pathological effects of inflammation. Here, we found that miR-33a-5p expression was significantly increased after TNF-α treatment during BMP-2-induced osteogenic differentiation of hBMSCs. Luciferase reporter assays and western blotting demonstrated that s...

journal_title：FEBS letters

authors： Mi W,Shi Q,Chen X,Wu T,Huang H

更新日期：2016-02-01 00:00:00

abstract：:Rat calvaria cells grown in culture for one week had properties of osteoblasts: a high content in alkaline phosphatase and a marked cyclic AMP response to parathyroid hormone (PTH). In short-term experiments, insulin-like growth factor I (IGF I) stimulated the incorporation of [14C] glucose into glycogen. When IGF I w...

journal_title：FEBS letters

authors： Schmid C,Steiner T,Froesch ER

更新日期：1984-07-23 00:00:00

abstract：:Two RNA silencing-related phenomena, quelling and meiotic silencing by unpaired DNA (MSUD) have been identified in the fungus Neurospora crassa. Similar to the case with the siRNA and miRNA pathways in Drosophila, different sets of protein components including RNA-dependent RNA polymerase, argonaute and dicer, are use...

journal_title：FEBS letters

authors： Nakayashiki H

更新日期：2005-10-31 00:00:00

abstract：:The C1 domains are 50 amino acid sequences present in protein kinase C (PKC) isozymes that are responsible for binding of phorbol esters and the lipid second messenger diacylglycerol (DAG). We found that bacterially expressed C1b domain of PKCdelta induces germinal vesicle breakdown (GVBD) when microinjected into Xeno...

journal_title：FEBS letters

authors： Aroca P,Santos E,Kazanietz MG

更新日期：2000-10-13 00:00:00

abstract：:We have purified two growth factors from dogfish brain and retina by using their binding affinity for heparin-Sepharose. These growth factors were eluted at 1 and 2 M NaCl similarly to those purified from bovine brain or retina. Their mitogenic activity was assayed in vitro on the same mammalian cells: bovine lens epi...

journal_title：FEBS letters

authors： Lagente O,Diry M,Courtois Y

更新日期：1986-07-07 00:00:00

abstract：:In the current work, we report that specific inhibition of Janus tyrosine kinase (JAK3) via NC1153 induces apoptosis of certain leukemia/lymphoma cell lines. Affymetrix microarray profiling following NC1153 treatment unveiled JAK3 dependent survival modulating pathways (p53, TGF-beta, TNFR and ER stress) in Kit225 cel...

journal_title：FEBS letters

authors： Nagy ZS,Ross JA,Rodriguez G,Bader J,Dimmock J,Kirken RA

更新日期：2010-04-16 00:00:00

abstract：:The fat mouse strain exhibits a late-onset obesity syndrome associated with a mutation in the gene encoding carboxypeptidase E (CPE). Since CPE plays a central role in the biosynthesis of a number of regulatory peptides, including gastrin, we examined the biogenesis and processing of progastrin in fat/fat mice by meas...

journal_title：FEBS letters

authors： Lacourse KA,Friis-Hansen L,Rehfeld JF,Samuelson LC

更新日期：1997-10-13 00:00:00

abstract：:A number of cDNA clones in summary encoding 700 amino acid residues from the N-end of rat liver elongation factor 2 (EF-2) and including 49 nucleotides of the 5'-untranslated mRNA region have been obtained. EF-2 cDNA clones were isolated from gradually constructed small (1000-5000 clones) specific cDNA libraries using...

journal_title：FEBS letters

authors： Oleinikov AV,Jokhadze GG,Alakhov YuB

更新日期：1989-05-08 00:00:00

abstract：:Octopus, squid and cuttle-fish organs were examined for alcohol dehydrogenase activity. Only one form was detectable, with properties typical of mammalian class III alcohol dehydrogenase. The corresponding protein was purified from octopus and enzymatically characterized. Ion-exchange and affinity chromatography produ...

journal_title：FEBS letters

authors： Rosario Fernández M,Jörnvall H,Moreno A,Kaiser R,Parés X

更新日期：1993-08-16 00:00:00

abstract：:Moloney murine leukemia virus reverse transcriptase (RT) selectively uses deoxyribonucleotides over ribonucleotides (rNTPs) as substrates. Substitution of F155 with valine (F155V) was previously found to increase the enzyme's affinity for rNTPs, though without affecting the V(max) for catalysis, and thereby conferred ...

journal_title：FEBS letters

authors： Liu S,Goff SP,Gao G

更新日期：2006-02-20 00:00:00

abstract：:Amino acid sequencing of a 48/46 kDa glycoprotein from human plantar callus, recognised by antisera raised against the desmosomal cadherins DGII/III, has revealed N-terminal homology to the DNA-derived sequence of human and bovine DGII/III. However, a tryptic fragment has homology only with a bovine clone. We propose ...

journal_title：FEBS letters

authors： King IA,Magee AI,Rees DA,Buxton RS

更新日期：1991-07-29 00:00:00

abstract：:Domain structures of porcine lung and testicular angiotensin-converting enzymes (ACE) were studied by radiation inactivation to test the hypothesis that lung ACE has two catalytic sites localized to discrete, structurally independent domains (the N- and C-domains) of approximately equal size. The minimum functional si...

journal_title：FEBS letters

authors： Sakaguchi H,Hirose S,Kume T,Hagiwara H

更新日期：1992-06-29 00:00:00

abstract：:In response to osmotic cell swelling, Intestine 407 cells react with a rapid and transient activation of phospholipase D (PLD). To investigate the role of PLD during the regulatory volume decrease, cells were treated with 1-butanol resulting in a depletion of PLD substrates. Activation of volume-regulated anion channe...

journal_title：FEBS letters

authors： Tomassen SF,van der Wijk T,de Jonge HR,Tilly BC

更新日期：2004-05-21 00:00:00

abstract：:Comparative allelotyping of the short arm of human chromosome 3 (3p) in four types of epithelial carcinomas was performed using an identical set of polymorphic markers. In total, 117 samples of non-papillary renal cell carcinoma (RCC), non-small cell lung carcinoma (NSCLC), carcinoma of uterine cervix (CC), and breast...

journal_title：FEBS letters

authors： Braga E,Pugacheva E,Bazov I,Ermilova V,Kazubskaya T,Mazurenko N,Kisseljov F,Liu J,Garkavtseva R,Zabarovsky E,Kisselev L

更新日期：1999-07-09 00:00:00

abstract：:Ovulation is a complex process initiated by the surge of the pituitary luteinizing hormone (LH) that provokes the expression of specific genes. We report herein the isolation and characterization of an ovulation-associated, ovary-specific novel isoform of epoxide hydrolase 2 (Ephx2), Ephx2C. This variant is exclusivel...

journal_title：FEBS letters

authors： Shkolnik K,Ben-Dor S,Galiani D,Hourvitz A,Dekel N

更新日期：2007-10-16 00:00:00

abstract：:The effect of all possible codons corresponding to the second and third amino acid (isoleucine and threonine) on the expression level of hirudin in E. coli has been analysed. These levels could not be correlated with changes in primary and secondary mRNA structure. A decrease in the rate of synthesis and of product ac...

journal_title：FEBS letters

authors： Degryse E

更新日期：1990-08-20 00:00:00

abstract：:Various cDNAs coding for part or all of human pre-pro lactase-phlorizin hydrolase (pre-proLPH) were transfected into COS cells and the subcellular location of the lactase-related proteins assessed. Only the complete proLPH reached the plasma membrane. LPH without the pro sequence, and a construct containing the pro se...

journal_title：FEBS letters

authors： Oberholzer T,Mantei N,Semenza G

更新日期：1993-10-25 00:00:00

abstract：:Regulation of acetylcholine receptor (AChR) gene expression was analyzed in alpha-bungarotoxin (alpha-BTX) treated rats. A reduction in available 125I-alpha-BTX binding sites was accompanied by an increase in the various AChR transcripts. The increase in the AChR alpha-, beta- epsilon- and delta-subunit mRNAs was simi...

journal_title：FEBS letters

authors： Asher O,Provenzano C,Fuchs S

更新日期：1991-05-06 00:00:00

abstract：:A highly specific and sensitive radioimmunoassay has been established for measurement of human endothelin (hET) in human plasma. After extraction of plasma with an octyl-silica column, this assay allowed for detection of immunoreactive (IR) hET as low as 0.2 fmol/ml. In 16 healthy subjects, the mean concentration of p...

journal_title：FEBS letters

authors： Ando K,Hirata Y,Shichiri M,Emori T,Marumo F

更新日期：1989-03-13 00:00:00

abstract：:Phosphoenolpyruvate carboxylases from various organisms contain two conserved lysine residues. In the C4 dicot Flaveria trinervia, one of these residues is Lys600. Converting this Lys600 to Arg600 or Thr600 mainly increased the Km values and but had minimal effect on the Vmax. The Km for PEP, Mg2+ increased by up to 3...

journal_title：FEBS letters

authors： Gao Y,Woo KC

更新日期：1995-11-13 00:00:00

abstract：:The archaeal exosome, a protein complex responsible for phosphorolytic degradation and tailing of RNA, has an RNA-binding platform containing Rrp4, Csl4, and DnaG. Aiming to detect novel interaction partners of the exosome, we copurified Nop5, which is a part of an rRNA methylating ribonucleoprotein complex, with the ...

journal_title：FEBS letters

authors： Gauernack AS,Lassek C,Hou L,Dzieciolowski J,Evguenieva-Hackenberg E,Klug G

更新日期：2017-12-01 00:00:00