Label-free optical biosensor with microfluidics for sensing ligand-directed functional selectivity on trafficking of thrombin receptor.


:Ligand-directed functional selectivity has stimulated much interest in the molecular delineation of drug pharmacology and the process of drug discovery. Here we describe the development of a label-free optical biosensor with microfluidics for whole cell sensing. The microfluidics controls the duration of agonist exposure and of the functional recovery of activated protease activated receptor-1 (PAR(1)). The biosensor manifests that the persistency of PAR(1) signaling is dependent on the agonists, which, in turn, affects the receptor resensitization process. This study demonstrates a new means to differentiate ligand-directed functional selectivity on trafficking of receptors.




FEBS letters


Goral V,Wu Q,Sun H,Fang Y




Has Abstract


2011-04-06 00:00:00














  • The bacteriochlorophyll biosynthesis gene, bchM, of Rhodobacter sphaeroides encodes S-adenosyl-L-methionine: Mg protoporphyrin IX methyltransferase.

    abstract::The bchM gene of Rhodobacter sphaeroides has been sequenced and then overexpressed in E. coli producing a protein of M(r) approximately. 27,500. Cell-free extracts of the transformed E. coli strain are able to methylate added Mg protoporphyrin, resulting in the formation of Mg protoporphyrin monomethyl ester. The iden...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Gibson LC,Hunter CN

    更新日期:1994-09-26 00:00:00

  • In vivo translational efficiency of different hepatitis C virus 5'-UTRs.

    abstract::Initiation of translation in hepatitis C virus (HCV) is dependent on the presence of an internal ribosome entry site (IRES) contained in its 341-nt-long 5'-untranslated region (UTR). This region is very conserved among different isolates and has been used to classify HCV isolates in six different genotypes. These geno...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Buratti E,Gerotto M,Pontisso P,Alberti A,Tisminetzky SG,Baralle FE

    更新日期:1997-07-14 00:00:00

  • NGF-dependent and tissue-specific transcription of vgf is regulated by a CREB-p300 and bHLH factor interaction.

    abstract::Neurotrophins support neuronal survival, development, and plasticity through processes requiring gene expression. We studied how vgf target gene transcription is mediated by a critical promoter region containing E-box, CCAAT and cAMP response element (CRE) sites. The p300 acetylase was present in two distinct protein ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Mandolesi G,Gargano S,Pennuto M,Illi B,Molfetta R,Soucek L,Mosca L,Levi A,Jucker R,Nasi S

    更新日期:2002-01-02 00:00:00

  • Esmolol is antiarrhythmic in doxorubicin-induced arrhythmia in cultured cardiomyocytes - determination by novel rapid cardiomyocyte assay.

    abstract::Cardiac toxicity is an uncommon but potentially serious complication of cancer therapy, especially with anthracyclines. One of the most effective anticancer drugs is doxorubicin, but its value is limited by the risk of developing cardiomyopathy and ventricular arrhythmia. When applied to a network of periodically cont...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Gorelik J,Vodyanoy I,Shevchuk AI,Diakonov IA,Lab MJ,Korchev YE

    更新日期:2003-07-31 00:00:00

  • Linkage between phosphorylated cystatin alpha and filaggrin by epidermal transglutaminase as a model of cornified envelope and inhibition of cathepsin L activity by cornified envelope and the conjugated cystatin alpha.

    abstract::Lysine-rich phosphorylated cystatin alpha (P-cystatin alpha) from newborn rat epidermis is a good substrate for epidermal transglutaminase (TGase) and also one of the component proteins of cornified envelope in the stratum corneum. Since the filaggrin linker segment peptide was efficiently conjugated with P-cystatin a...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Takahashi M,Tezuka T,Kakegawa H,Katunuma N

    更新日期:1994-03-07 00:00:00

  • On the substrate specificity of nitric oxide synthase.

    abstract::Nitric oxide (.NO) synthase (NOS) activity in subcellular fractions from cultured endothelial cells (EC) and lipopolysaccharide-activated J774.2 monocyte/macrophages was investigated by monitoring the .NO-mediated increase in intracellular cyclic GMP in LLC-PK1 pig kidney epithelial cells. The constitutive NOS in EC (...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Hecker M,Walsh DT,Vane JR

    更新日期:1991-12-09 00:00:00

  • Autophosphorylation of the two C-terminal tyrosine residues Tyr1316 and Tyr1322 modulates the activity of the insulin receptor kinase in vitro.

    abstract::Previously, several studies have demonstrated that autophosphorylation of the C-terminal tyrosine residues (Tyr1316 and Tyr1322) affects the signaling properties of the insulin receptor in vivo. To assess the biochemical consequences of the C-terminal phosphorylation in vitro, we have constructed, purified and charact...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Tennagels N,Bergschneider E,Al-Hasani H,Klein HW

    更新日期:2000-08-11 00:00:00

  • Mode of action of bottromycin A2: effect of bottromycin A2 on polysomes.

    abstract::When bottromycin A2 was added to an in vitro protein synthesis system carried out by naturally occurring polysomes, it inhibited protein synthesis effectively. Examination of the 3 steps of peptide chain elongation revealed that the binding of aminoacyl-tRNA to the polyribosomes was inhibited by bottromycin A2. In con...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Otaka T,Kaji A

    更新日期:1983-03-07 00:00:00

  • Novel bioactive lipodepsipeptides from Pseudomonas syringae: the pseudomycins.

    abstract::The covalent structure and most of the stereochemistry of the pseudomycins, bioactive metabolites of a transposon-generated mutant of a Pseudomonas syringae wild-type strain proposed for the biological control of Dutch elm disease, have been determined. While two pseudomycins are identical to the known syringopeptins ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Ballio A,Bossa F,Di Giorgio D,Ferranti P,Paci M,Pucci P,Scaloni A,Segre A,Strobel GA

    更新日期:1994-11-21 00:00:00

  • In situ dephosphorylation of tau by protein phosphatase 2A and 2B in fetal rat primary cultured neurons.

    abstract::Using antibodies recognizing the phosphorylation state of specific sites, phosphorylation states of tau were monitored in fetal rat primary cultured neurons. When cultured neurons were treated with okadaic acid (OA) or calyculin A (CalA) at concentrations sufficient to inhibit protein phosphatase 2A (PP2A), phosphoryl...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Saito T,Ishiguro K,Uchida T,Miyamoto E,Kishimoto T,Hisanaga S

    更新日期:1995-12-04 00:00:00

  • N-terminal sequence homologies in interstitial retinol-binding proteins from 10 vertebrate species.

    abstract::We report here the first comprehensive comparative NH2-terminal sequence studies of interstitial retinol-binding protein (IRBPs) from nine mammals (including cattle) and one amphibian. This study has revealed that in many species the N-terminus of IRBP includes a 3-6 amino acid extension. IRBP possessing this leader s...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Fong SL,Cook RG,Alvarez RA,Liou GI,Landers RA,Bridges CD

    更新日期:1986-09-15 00:00:00

  • Expression of 9 Salmonella typhimurium enzymes for cobinamide synthesis. Identification of the 11-methyl and 20-methyl transferases of corrin biosynthesis.

    abstract::Nine of the cbi genes from the 17.5 kb cob operon of Salmonella typhimurium previously shown by genetic studies to be involved in the biosynthesis of cobinamide from precorrin-2, have been subcloned and expressed in Escherichia coli. Seven of the gene products were found in the soluble fraction of cell lysates and hav...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Roessner CA,Warren MJ,Santander PJ,Atshaves BP,Ozaki S,Stolowich NJ,Iida K,Scott AI

    更新日期:1992-04-13 00:00:00

  • Expression of human apolipoprotein A-I/C-III/A-IV gene cluster in mice reduces atherogenesis in response to a high fat-high cholesterol diet.

    abstract::We have previously generated transgenic (Tg) mice expressing the human apolipoprotein (apo) A-I/C-III/A-IV gene cluster. This expression induced hyperlipidemia but reduced atherosclerotic lesions in genetically modified mice lacking apoE. Atherosclerosis is a multifactorial process and environmental factors such as di...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Baroukh N,Ostos MA,Vergnes L,Recalde D,Staels B,Fruchart J,Ochoa A,Castro G,Zakin MM

    更新日期:2001-07-27 00:00:00

  • Pyrimidine tract binding protein and La autoantigen interact differently with the 5' untranslated regions of lentiviruses and oncoretrovirus mRNAs.

    abstract::Retrovirus genomic mRNA exhibits a several hundred nucleotides-long untranslated region (5' UTR) which encloses many control elements required for retrovirus replication. In addition, this 5' UTR contains translation regulatory elements, such as internal ribosome entry sites (IRESes) that have been described in oncore...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Waysbort A,Bonnal S,Audigier S,Estève JP,Prats AC

    更新日期:2001-02-09 00:00:00

  • Cell fate determination, neuronal maintenance and disease state: The emerging role of transcription factors Lmx1a and Lmx1b.

    abstract::LIM-homeodomain (LIM-HD) proteins are evolutionary conserved developmental transcription factors. LIM-HD Lmx1a and Lmx1b orchestrate complex temporal and spatial gene expression of the dopaminergic pathway, and evidence shows they are also involved in adult neuronal homeostasis. In this review, the multiple roles play...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Doucet-Beaupré H,Ang SL,Lévesque M

    更新日期:2015-12-21 00:00:00

  • A novel human UDP-N-acetyl-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase, GalNAc-T7, with specificity for partial GalNAc-glycosylated acceptor substrates.

    abstract::A novel member of the human UDP-N-acetyl-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase gene family, designated GalNAc-T7, was cloned and expressed. GalNAc-T7 exhibited different properties compared to other characterized members of this gene family, in showing apparent exclusive specificity for partial...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Bennett EP,Hassan H,Hollingsworth MA,Clausen H

    更新日期:1999-10-29 00:00:00

  • Identification and purification of a 36 kDa bile acid binder in human hepatic cytosol.

    abstract::We recently purified two closely related 33 kDa proteins from rat hepatic cytosol, designated bile acid binder I and II, which selectively bind bile acids with comparable affinity as glutathione S-transferase B. This work has now been extended to human liver in which we have identified a similar cytosolic binding acti...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Stolz A,Sugiyama Y,Kuhlenkamp J,Kaplowitz N

    更新日期:1984-11-05 00:00:00

  • Two-dimensional gel electrophoresis and FTIR spectroscopy reveal both forms of yeast plasma membrane H(+)-ATPase in activated and basal-level enzyme preparations.

    abstract::Plasma membrane H(+)-ATPase of the yeast Saccharomyces cerevisiae was isolated and purified in its two forms, the activated A-ATPase from glucose-metabolizing cells, and the basal-level B-ATPase from cells with endogenous metabolism only. Using two-dimensional gel electrophoretic analysis, we showed that both enzyme p...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Lapathitis G,Tanfani F,Kotyk A,Bertoli E

    更新日期:2001-09-07 00:00:00

  • Expression of protein kinase C isozymes in hippocampal neurones in culture.

    abstract::Several protein kinase C (PKC) isozymes were analyzed by immunoblot and immunocytochemistry in cultures of hippocampal neurones at several stages of differentiation. Our findings reveal the existence of two distinct patterns of expression. Firstly, conventional PKC isozymes alpha, beta and gamma, that are expressed at...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Tejero-Díez P,Rodríguez-Sánchez P,Díez-Guerra FJ

    更新日期:1995-04-24 00:00:00

  • Oxygen induces fatty acid (n-6)-desaturation independently of temperature in Acanthamoeba castellanii.

    abstract::Induction of a microsomal oleate delta12 (n-6) desaturase which is mainly responsible for an increase in membrane lipid unsaturation at low temperature has been observed in the free-living amoeba Acanthamoeba castellanii. In this study we show that the enzyme can also be regulated by oxygen independently of temperatur...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Thomas K,Rutter A,Suller M,Harwood J,Lloyd D

    更新日期:1998-03-20 00:00:00

  • Essential structure for full enterotoxigenic activity of heat-stable enterotoxin produced by enterotoxigenic Escherichia coli.

    abstract::Several analogues of heat-stable enterotoxins (STh and STp) produced by enterotoxigenic Escherichia coli were synthesized. Peptides (STh[6-18] and STp[5-17]) consisting of 13 amino acid residues from the Cys residue near the N-terminus to the Cys residue near the C-terminus and linked by three disulfide bonds had the ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Yoshimura S,Ikemura H,Watanabe H,Aimoto S,Shimonishi Y,Hara S,Takeda T,Miwatani T,Takeda Y

    更新日期:1985-02-11 00:00:00

  • Two distinct classes of rat intestinal mucosal enzymes incorporating putrescine into protein.

    abstract::Tissue-transglutaminase (t-TGase) is a family of calcium-dependent enzymes. A Ca2+-independent soluble enzyme, in addition to t-TGase, capable of incorporating polyamines into proteins was demonstrated in rat intestinal mucosa. The Ca2+-independent enzyme was stimulated 2- to 5-fold by Fe2+ and Co2+ ions but inhibited...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Tsai YH,Lai WF,Wu YW,Johnson LR

    更新日期:1998-09-18 00:00:00

  • Solubilisation partial characterisation of the alpha-MSH receptor on primary rat Schwann cells.

    abstract::The ACTH/MSH melanocortin core peptide sequence possesses neurotrophic properties in peripheral nerve. During functional neuroanatomical recovery after damage to peripheral nerves, Schwann cells play a significant role in facilitating regeneration. Here we employ a modified super-potent alpha-MSH analogue to solubilis...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Dyer JK,Ahmed AR,Oliver GW,Poulton CW,Haynes LW

    更新日期:1993-12-20 00:00:00

  • Amphipathic polyethyleneglycols effectively prolong the circulation time of liposomes.

    abstract::Incorporation of dioleoyl N-(monomethoxy polyethyleneglycol succinyl)phosphatidylethanolamine (PEG-PE) into large unilamellar liposomes composed of egg phosphatidylcholine:cholesterol (1:1) does not significantly increase the content leakage when the liposomes are exposed to 90% human serum at 37 degrees C, yet the li...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Klibanov AL,Maruyama K,Torchilin VP,Huang L

    更新日期:1990-07-30 00:00:00

  • Thimet oligopeptidase: site-directed mutagenesis disproves previous assumptions about the nature of the catalytic site.

    abstract::Zinc metallopeptidases that contain the His-Glu-Xaa-Xaa-His (HEXXH) motif generally have a third ligand of the metal ion that may be either a Glu residue (in clan MA) or a His residue (in clan MB) (Rawlings and Barrett (1995) Methods Enzymol. 248, 183-228). Thimet oligopeptidase has not yet been assigned to either cla...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Chen JM,Stevens RA,Wray PW,Rawlings ND,Barrett AJ

    更新日期:1998-09-11 00:00:00

  • Binding of progastrin fragments to the 78 kDa gastrin-binding protein.

    abstract::The non-selective gastrin/cholecystokinin receptor antagonists proglumide and benzotript inhibit colon carcinoma cell proliferation by binding to the 78 kDa gastrin-binding protein (GBP) (Baldwin, Proc. Natl. Acad. Sci. USA, 91 (1994) 7593-7597). However, although most colon carcinoma cell lines synthesize progastrin,...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Baldwin GS

    更新日期:1995-02-06 00:00:00

  • Increasing cAMP antagonizes hypertrophic response to angiotensin II without affecting Ras and MAP kinase activation in vascular smooth muscle cells.

    abstract::Angiotensin II (Ang II), a potent hypertrophic factor for vascular smooth muscle cells (VSMC), induces activation of the ras proto-oncogene product (Ras) and mitogen-activated protein (MAP) kinases, and tyrosine phosphorylation of a focal adhesion-associated protein, paxillin. Forskolin, a direct activator of adenylat...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Takahashi T,Kawahara Y,Okuda M,Yokoyama M

    更新日期:1996-11-11 00:00:00

  • Fine tuning of cell functions through remodeling of glycosphingolipids by plasma membrane-associated glycohydrolases.

    abstract::The plasma membrane (PM) sphingolipid composition is the result of a series of well-known metabolic pathways comprising neobiosynthesis in the endoplasmic reticulum and in the Golgi apparatus followed by vesicular delivery to the plasma membrane, membrane turnover with final catabolism in lysosomes, and shedding of me...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Sonnino S,Aureli M,Loberto N,Chigorno V,Prinetti A

    更新日期:2010-05-03 00:00:00

  • Heterogeneous turnover of terminal and core sugars within the carbohydrate chain of dipeptidylaminopeptidase IV isolated from rat liver plasma membrane.

    abstract::Dipeptidylaminopeptidase IV, a plasma membrane-bound glycoprotein, is characterized by an intramolecular heterogeneous turnover of the protein backbone and carbohydrate chain. The faster turnover of the latter is restricted only to the outer sugars. The inner core sugars D-mannose and N-acetyl-D-glucosamine turn over ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Volk BA,Kreisel W,Köttgen E,Gerok W,Reutter W

    更新日期:1983-10-31 00:00:00

  • Multiple tRNA attachment sites in prothymosin alpha.

    abstract::A covalent complex formed by bacterial tRNAs and prothymosin alpha, an abundant acidic nuclear protein involved in proliferation of mammalian cells, upon production of the recombinant rat protein in Escherichia coli cells was studied. Several tRNA attachment sites were identified in the prothymosin alpha molecule usin...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Lukashev DE,Chichkova NV,Vartapetian AB

    更新日期:1999-05-21 00:00:00