Label-free optical biosensor with microfluidics for sensing ligand-directed functional selectivity on trafficking of thrombin receptor.

Abstract:

:Ligand-directed functional selectivity has stimulated much interest in the molecular delineation of drug pharmacology and the process of drug discovery. Here we describe the development of a label-free optical biosensor with microfluidics for whole cell sensing. The microfluidics controls the duration of agonist exposure and of the functional recovery of activated protease activated receptor-1 (PAR(1)). The biosensor manifests that the persistency of PAR(1) signaling is dependent on the agonists, which, in turn, affects the receptor resensitization process. This study demonstrates a new means to differentiate ligand-directed functional selectivity on trafficking of receptors.

journal_name

FEBS Lett

journal_title

FEBS letters

authors

Goral V,Wu Q,Sun H,Fang Y

doi

10.1016/j.febslet.2011.03.003

subject

Has Abstract

pub_date

2011-04-06 00:00:00

pages

1054-60

issue

7

eissn

0014-5793

issn

1873-3468

pii

S0014-5793(11)00153-0

journal_volume

585

pub_type

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