Abstract:
:A number of cDNA clones in summary encoding 700 amino acid residues from the N-end of rat liver elongation factor 2 (EF-2) and including 49 nucleotides of the 5'-untranslated mRNA region have been obtained. EF-2 cDNA clones were isolated from gradually constructed small (1000-5000 clones) specific cDNA libraries using the primer extension method for synthesis of the first cDNA chain. The complete primary structure of cDNA and protein EF-2 from rat liver was derived taking into account the primary structure of the 3'-terminal region EF-2 cDNA previously reported [(1986) Proc. Natl. Acad. Sci. USA 83, 4978-4982]. Comparison of this cDNA with hamster cDNA has shown that (i) the base sequences had a 89.7% homology while that of the 5'-untranslated region was 73%; (ii) there are two amino acid replacement in rat liver EF-2 as compared with hamster EF-2.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Oleinikov AV,Jokhadze GG,Alakhov YuBdoi
10.1016/0014-5793(89)80447-8subject
Has Abstractpub_date
1989-05-08 00:00:00pages
131-6issue
1-2eissn
0014-5793issn
1873-3468journal_volume
248pub_type
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