Role of the C-terminal domain of Bax and Bcl-XL in their localization and function in yeast cells.

abstract：:Ischemia/reperfusion induces nuclear factor kappaB (NF-kappaB) and AP-1 in rat hearts after 15 min of ischemia followed by reperfusion (R) for various periods of time (15 and 30 min, 1, 2, 3, 6, 12, and 24 h). Low levels of NF-kappaB and no signal for AP-1 were detected in shams and in non-ischemic tissue distant from...

journal_title：FEBS letters

authors： Chandrasekar B,Freeman GL

更新日期：1997-01-13 00:00:00

abstract：:To identify suitable promoters for expressing exogenous genes in arthritic joints, the constitutive, simian virus 40 (SV 40) and IL-1 or metal inducible, human stromelysin and metallothionein (MT) gene promoters were tested for their activity in chondrocytes and synovial fibroblasts. Transient transfection with plasmi...

journal_title：FEBS letters

authors： Su S,Gedamu L,Dehnade F,Zafarullah M

更新日期：1996-10-21 00:00:00

abstract：:Ca2+-dependent chromatography of soluble cytosolic proteins on calmodulin-Sepharose gave a fraction that exhibited Ca2+- and calmodulin-dependent phosphorylation of several polypeptides, including 60, 56 and 45 kDa species. At 0.2 microM beef calmodulin the phosphorylation was optimal at 3 microM free Ca2+, and at 80 ...

journal_title：FEBS letters

authors： Londesborough J,Nuutinen M

更新日期：1987-07-13 00:00:00

abstract：:Sucrose synthase (SuSy) plays an important role in sucrose degradation and occurs both as a soluble and as a membrane-associated enzyme in higher plants. We show that membrane association can vary in vivo in response to gravistimulation, apparently involving SuSy dephosphorylation, and is a reversible process in vitro...

journal_title：FEBS letters

authors： Winter H,Huber JL,Huber SC

更新日期：1997-12-29 00:00:00

abstract：:The Type I cGMP-dependent protein kinase catalytic domain (residues 336-671 from the I alpha isoform) has been expressed as a cGMP independent kinase in a baculovirus system. Using peptide substrates, the protein retains similar substrate specificity as the native holoenzyme. The recombinant catalytic domain catalyzes...

journal_title：FEBS letters

authors： Boerth NJ,Lincoln TM

更新日期：1994-04-11 00:00:00

abstract：:An interpretation of alternative crystal structures of the erythropoietin receptor, with and without ligand, led to the proposal of a scissor-like mechanism of activation. This model has been propagated in the literature and is still being used to interpret crystal structures of related type-I cytokine receptors. Here...

journal_title：FEBS letters

authors： Corbett MS,Poger D,Mark AE

更新日期：2016-09-01 00:00:00

abstract：:Cellular homeostasis in response to glucose availability is maintained through the tight coordination of various physiological processes, including cell proliferation, transcription, and metabolism. In this study, we use the budding yeast Saccharomyces cerevisiae to identify proteins implicated in carbon source-depend...

journal_title：FEBS letters

authors： Umekawa M,Shiraishi D,Fuwa M,Sawaguchi K,Mashima Y,Katayama T,Karita S

更新日期：2020-04-01 00:00:00

abstract：:Increases in choline containing metabolites have been associated with a number of disorders, including malignant cell growth. In this study, high resolution magic angle spinning (1)H nuclear magnetic resonance spectroscopy was employed to monitor metabolite changes during cell transfection, and an increase in phosphoc...

journal_title：FEBS letters

authors： Griffin JL,Mann CJ,Scott J,Shoulders CC,Nicholson JK

更新日期：2001-12-07 00:00:00

abstract：:In adipogenesis, growth factors play a crucial role. Using serum depleted condition, we studied the causal role of endothelin-1 (ET-1) and epidermal growth factor (EGF), separately or together, in adipocyte differentiation of 3T3-L1 cells. ET-1 stimulation caused an anti-adipogenic response and this effect was potenti...

journal_title：FEBS letters

authors： Bhattacharya I,Ullrich A

更新日期：2006-10-16 00:00:00

abstract：:Frame shift mutations of the polyglutamine binding protein-1 (PQBP1) gene lead to total or partial truncation of the C-terminal domain (CTD) and cause mental retardation in human patients. Interestingly, normal Drosophila homologue of PQBP-1 lacks CTD. As a model to analyze the molecular network of PQBP-1 affecting in...

journal_title：FEBS letters

authors： Yoshimura N,Horiuchi D,Shibata M,Saitoe M,Qi ML,Okazawa H

更新日期：2006-04-17 00:00:00

abstract：:Extended X-ray absorption fine structure (EXAFS) spectra at the Cu K-edge have been recorded of the oxidized and reduced form at pH 3.5 of rusticyanin, the type-1 or 'blue'-copper protein from Thiobacillus ferrooxidans. The EXAFS of oxidized rusticyanin is well simulated with models assuming a ligand set of 2 N(His) a...

journal_title：FEBS letters

authors： Holt SD,Piggott B,Ingledew WJ,Feiters MC,Diakun GP

更新日期：1990-08-20 00:00:00

abstract：:Read-through or mutations of a stop codon resulting in translation of the 3'-UTR produce potentially toxic C-terminally extended proteins. However, quality control mechanisms for such proteins are poorly understood in mammalian cells. Here, a comprehensive analysis of the 3'-UTRs of genes associated with hereditary di...

journal_title：FEBS letters

authors： Hashimoto S,Nobuta R,Izawa T,Inada T

更新日期：2019-04-01 00:00:00

abstract：:Pore-forming toxins, such as colicin A, are water-soluble proteins that insert into lipid bilayers. The water-soluble structure of Colicin A is known at a high resolution and this review describes the kinetic and structural steps involved in its soluble-to-membrane bound transformation. ...

journal_title：FEBS letters

authors： Lakey JH,González-Mañas JM,van der Goot FG,Pattus F

更新日期：1992-07-27 00:00:00

abstract：:The chemotherapeutic activity of three synthetic antibacterial peptides was investigated. KLKLLLLLKLK-NH2 and its D-enantiomer showed significant chemotherapeutic activity in MRSA-infected mice, whereas KLKLLLKLK-NH2, which showed the highest antibacterial activity among them in vitro, was found to have almost no abil...

journal_title：FEBS letters

authors： Nakajima Y,Alvarez-Bravo J,Cho J,Homma K,Kanegasaki S,Natori S

更新日期：1997-09-22 00:00:00

abstract：:A homogeneous protein with a subunit apparent molecular mass of ∼50 kDa that catalyzes the previously described mitochondrial NADH-supported ammonium-stimulated hydrogen peroxide production (Grivennikova, V.G., Gecchini, G. and Vinogradov, A.D. (2008) FEBS Lett. 583, 1287-1291) was purified from the mitochondrial matr...

journal_title：FEBS letters

authors： Kareyeva AV,Grivennikova VG,Cecchini G,Vinogradov AD

更新日期：2011-01-21 00:00:00

abstract：:The early embryonic development of Xenopus is mainly governed by post-transcriptional regulations until the mid-blastula transition. In this report, we present evidence demonstrating that fertilization of Xenopus eggs triggers a complete translational arrest of mRNAs containing UA-rich elements in their 3'-untranslate...

journal_title：FEBS letters

authors： Marinx O,Bertrand S,Karsenti E,Huez G,Kruys V

更新日期：1994-05-30 00:00:00

abstract：:We constructed five mutated cDNAs encoding human interferon-gamma (IFN-gamma) derivatives lacking 19-23 COOH-terminal residues and expressed them in Escherichia coli. All the derivatives were purified to homogeneity. They showed substantially the same order of antiviral activity in vitro as the intact molecule, and be...

journal_title：FEBS letters

authors： Sakaguchi M,Honda S,Ozawa M,Nishimura O

更新日期：1988-03-28 00:00:00

abstract：:FabH (beta-ketoacyl-acyl carrier protein synthase III) is unique in that it initiates fatty acid biosynthesis, is inhibited by long-chain fatty acids providing means for feedback control of the process, and dictates the fatty acid profile of the organism by virtue of its substrate specificity. We report the crystal st...

journal_title：FEBS letters

authors： Gajiwala KS,Margosiak S,Lu J,Cortez J,Su Y,Nie Z,Appelt K

更新日期：2009-09-03 00:00:00

abstract：:Michaelis-Menten kinetics of glycerol-3-phosphate dehydrogenase activity in proteoliposomes from brown adipose tissue mitochondria with exogenously added phospholipids or cholesterol was measured. It was shown that changes in membrane lipid composition affected the membrane lateral pressure and therefore modulated the...

journal_title：FEBS letters

authors： Amler E,Jasińska R,Drahota Z,Zborowski J

更新日期：1990-10-01 00:00:00

abstract：:Mechanisms underlying the association between fibroblastic growth factor 23 (FGF-23) and inflammation are uncertain. We found that FGF-23 was markedly up-regulated in LPS/INF-γ-induced proinflammatory M1 macrophages and Hyp mouse-derived peritoneal macrophages, but not in IL-4-induced M2 anti-inflammatory macrophages....

journal_title：FEBS letters

authors： Han X,Li L,Yang J,King G,Xiao Z,Quarles LD

更新日期：2016-01-01 00:00:00

abstract：:A mouse cDNA encoding for matrilin-3, the third member of the novel matrilin family of extracellular matrix proteins, was cloned. The protein precursor of 481 amino acids consists of a putative signal peptide, a short positively charged sequence, a single vWFA-like domain followed by four epidermal growth factor-like ...

journal_title：FEBS letters

authors： Wagener R,Kobbe B,Paulsson M

更新日期：1997-08-11 00:00:00

abstract：:We have determined the first de novo position of the secondary quinone QB in the Rhodobacter sphaeroides reaction center (RC) using phases derived by the single wavelength anomalous dispersion method from crystals with selenomethionine substitution. We found that in frozen RC crystals, QB occupies primarily the proxim...

journal_title：FEBS letters

authors： Pokkuluri PR,Laible PD,Crawford AE,Mayfield JF,Yousef MA,Ginell SL,Hanson DK,Schiffer M

更新日期：2004-07-16 00:00:00

abstract：:Proteasomes were purified from human hepatoma tissues, and their sensitivities to Na+ and K+ were examined. At concentrations of 10 mM or more, these cations were found to inhibit completely polylysine-activated casein degradation by the purified proteasomes. They also strongly inhibited the hydrolyses of peptides, al...

journal_title：FEBS letters

authors： Seol JH,Park SC,Ha DB,Chung CH,Tanaka K,Ichihara A

更新日期：1989-04-24 00:00:00

abstract：:The specific binding of [3H]retinoids to cellular retinoid-binding proteins was measured directly by the cold acetone filtration method. After incubation of purified cellular retinoid-binding proteins with [3H]retinoids with or without competitors for 2-4 h, bound ligands were separated from free by filtration using c...

journal_title：FEBS letters

authors： Ninomiya M,Suganuma M,Paik NS,Muto Y,Fujiki H

更新日期：1988-06-20 00:00:00

abstract：:The farnesoid X receptor (FXR) is a member of the nuclear receptor superfamily that is primarily expressed in the enterohepatic system where it functions as intracellular sensor for bile acids. Ligand dependent FXR activation induces transcriptional responses to coordinately regulate bile acid, cholesterol, triglyceri...

journal_title：FEBS letters

authors： Modica S,Moschetta A

更新日期：2006-10-09 00:00:00

abstract：:Type II collagen is the major protein of cartilage and is synthesized as a procollagen in two forms (IIA and IIB), generated by differential splicing of the gene primary transcript. Previous studies have indicated that only type IIB is expressed in differentiated chondrocytes. Here, we examined the effects of bone mor...

journal_title：FEBS letters

authors： Valcourt U,Gouttenoire J,Aubert-Foucher E,Herbage D,Mallein-Gerin F

更新日期：2003-06-19 00:00:00

abstract：:Data obtained from peptide mapping of the active and inactive forms of human renin show that there are extensive regions of common sequence in the two forms of the enzyme, and are consistent with the hypothesis that inactive renin is a renin zymogen. ...

journal_title：FEBS letters

authors： McIntyre GD,Chaudhuri S,Coggins JR

更新日期：1984-10-01 00:00:00

abstract：:Glial cell line-derived neurotrophic factor (GDNF) and its canonical receptor Ret can signal together or independently to fulfill many important functions in the midbrain dopaminergic (DA) system. While Ret signaling clearly impacts on the development, maintenance and regeneration of the mesostriatal DA system, the ph...

journal_title：FEBS letters

authors： Kramer ER,Liss B

更新日期：2015-12-21 00:00:00

abstract：:Phosphatidylglycerophosphate (PGP) synthase, encoded by PGP1 and PGP2 in Arabidopsis, catalyzes a committed step in the biosynthesis of phosphatidylglycerol (PG). In this study, we isolated a pgp1pgp2 double mutant of Arabidopsis to study the function of PG. In this mutant, embryo development was delayed and the major...

journal_title：FEBS letters

authors： Tanoue R,Kobayashi M,Katayama K,Nagata N,Wada H

更新日期：2014-05-02 00:00:00

abstract：:Expression of the leptin receptor gene has been examined in mouse hypothalamus and other brain regions by in situ hybridization. With a probe recognizing all the known splice variants, receptor mRNA was evident in several brain regions (cortex, hippocampus, thalamus), with strong expression in the hypothalamus (arcuat...

journal_title：FEBS letters

authors： Mercer JG,Hoggard N,Williams LM,Lawrence CB,Hannah LT,Trayhurn P

更新日期：1996-06-03 00:00:00