Abstract:
:Those aerobic archaea whose genomes have been sequenced possess a single 4-gene operon that, by sequence comparisons with Bacteria and Eukarya, appears to encode the three component enzymes of a 2-oxoacid dehydrogenase multienzyme complex. However, no catalytic activity of any such complex has ever been detected in the Archaea. In the current paper, we have cloned and expressed the first two genes of this operon from the thermophilic archaeon, Thermoplasma acidophilum. We demonstrate that the protein products form an alpha2beta2 hetero-tetramer possessing the decarboxylase catalytic activity characteristic of the first component enzyme of a branched-chain 2-oxoacid dehydrogenase multienzyme complex. This represents the first report of the catalytic function of these putative archaeal multienzyme complexes.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Heath C,Jeffries AC,Hough DW,Danson MJdoi
10.1016/j.febslet.2004.10.058keywords:
subject
Has Abstractpub_date
2004-11-19 00:00:00pages
523-7issue
3eissn
0014-5793issn
1873-3468pii
S0014579304013122journal_volume
577pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::A novel gene encoding a new H3 histone varian (H3L) has been identified in P. lividus sea urchin embryo. It encodes a H3 histone protein showing the S.//.A.IG amino acid motif typical of the replication independent H3.3 variants but in a mRNA showing the 3' terminal stem-loop nucleotide sequence that is typical of the...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)00305-0
更新日期:1997-04-21 00:00:00
abstract::Leaves and chloroplasts from Arabidopsis plants with increased amounts of PsbS protein showed the same percentage increase in nonphotochemical quenching in comparison to the wild type both in the presence and absence of zeaxanthin. The absorption change at 525-535 nm was also more pronounced in both cases. It is sugge...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.03.005
更新日期:2006-04-03 00:00:00
abstract::Sulfated glycoprotein 2 (SGP-2) mRNA progressively increased in the ventral prostate of the aging rat, reaching, at 24 months, 4-fold higher than at 3 months. Ornithine decarboxylase (ODC) mRNA peaked at 6 months (4-fold increase), and at 12 and 24 months was maintained at higher levels than at 3 months. ODC enzymatic...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)00609-1
更新日期:1994-07-18 00:00:00
abstract::The mechanism underlying lipopolysaccharide (LPS)-induced depression of phosphoinositide (PI) hydrolysis was investigated using rat aortas. In LPS-pretreated aortas, the 5-hydroxytryptamine-stimulated accumulation of inositol monophosphate and incorporation of exogenous myo-inositol into PIs were significantly less th...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)02747-3
更新日期:2002-05-22 00:00:00
abstract::Treatment of cultured carrot cells with dibutyryl cAMP or forskolin resulted in the appreciable decrease in extracellular K+ concentration. This decrease was found to be transient and the concentration of the ion in the culture medium restored to the original level within few minutes. The cAMP-induced decrease in K+ l...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)00403-1
更新日期:1997-05-12 00:00:00
abstract::The regulatory system of smooth muscle thin filaments is thought to involve a major calcium-calmodulin and actin binding protein: caldesmon. A dissective approach was used to isolate a 35 kDa C-terminal fragment of the molecule and to produce antibodies reacting against both the intact and the 15 kDa N-terminal end of...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)81150-m
更新日期:1990-08-20 00:00:00
abstract::In cultured rat pituitary gonadotrophs, gonadotropin-releasing hormone (GnRH) induces rapid hyperpolarization of the cell membrane and causes cessation of the spontaneous electrical activity present in non-stimulated cells. This initial response to GnRH is followed by slow oscillations of membrane potential (Vm) which...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80201-q
更新日期:1992-04-13 00:00:00
abstract::PMA-induced leukotriene C4 synthase (LTC4S) phosphorylation was investigated over a period of 8 h in a monocytic cell line (THP-1). The level of LTC4S phosphorylation was increased 3-5 fold over a 4 h period decreasing to basal levels after 8 h. This phosphorylation event was found to be specific to THP-1 cells as the...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00397-x
更新日期:1999-04-16 00:00:00
abstract::In brain tissue a spectrin-like calmodulin-binding protein calspectin, or fodrin, is concentrated in a synaptosome fraction, where most of the calspectin is associated with the synaptic membranes. This endogenous calspectin was phosphorylated by protein kinase system(s) associated with the membranes. Here, we report t...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(82)81331-8
更新日期:1982-12-13 00:00:00
abstract::Mitochondrial creatine kinase (MiCK) occurs primarily as an octameric form localized in the mitochondrial intermembrane compartment in vertebrate tissues and echinoderm spermatozoa (both deuterostome groups). The octameric quaternary structure is thought to play important functional and enzyme targeting roles. We have...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)00204-x
更新日期:1998-03-20 00:00:00
abstract::The oleosin glycine-rich protein genes Atgrp-6, Atgrp-7, and Atgrp-8 occur in clusters in the Arabidopsis genome and are expressed specifically in the tapetum cells. The cis-regulatory regions involved in the tissue-specific gene expression were investigated by fusing different segments of the gene cluster to the uidA...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)02557-7
更新日期:2002-04-24 00:00:00
abstract::We examined the spatial expression patterns of two orphan receptor guanylyl cyclase genes OlGC4 and OlGC5 during embryogenesis of medaka and characterized the 5' flanking region required for tissue-specific expression of OlGC4 by introducing promoter-GFP fusion constructs into medaka embryos. Expression of OlGC5 is co...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)02109-8
更新日期:2000-10-20 00:00:00
abstract::The ability of (S)-alpha-methylproline (alpha-MePro) to stabilise reverse-turn conformations in the peptide hormone bradykinin (BK = Arg1-Pro2-Pro3-Gly4-Phe5-Ser6-Pro7-Phe8-Arg9) has been investigated. Two BK analogues containing alpha-MePro at position 3 or position 7 were synthesised and their conformations in aqueo...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80541-n
更新日期:1992-02-10 00:00:00
abstract::Cellular ageing is characterized by the accumulation of oxidatively modified proteins which may be due to increased protein damage and/or decreased elimination of oxidized protein. Since the proteasome is in charge of protein turnover and removal of oxidized protein, its fate during ageing and upon oxidative stress ha...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2006.03.028
更新日期:2006-05-22 00:00:00
abstract::Incubation of isolated rat liver nuclei with ATP, NAD+, and micromolar Ca2+ concentrations of various metal ions resulted in extensive DNA hydrolysis. Half-maximal activity occurred with 1.0 microM Ca2+ added, and saturation of the process was observed with 10 microM Ca2+. The Ca2+ (10 microM)-activated DNA fragmentat...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80168-3
更新日期:1991-02-25 00:00:00
abstract::Melittin, deuteromethylated on each of the four amino groups (Gly-1 N alpha and Lys-7, 21, and 23 N epsilon), was prepared by reductive methylation using deuteroformaldehyde and NaBD3CN. Deuterium NMR spectra were obtained for the modified peptide (D-melittin) bound to phospholipid bilayers and erythrocyte ghosts. D-M...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(87)81041-4
更新日期:1987-06-22 00:00:00
abstract::Myeloperoxidase (MPO) is the most abundant protein in neutrophils and plays a central role in microbial killing and inflammatory tissue damage. Because most of the non-steroidal anti-inflammatory drugs and other drugs contain a thiol group, it is necessary to understand how these substrates are oxidized by MPO. We hav...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)01727-x
更新日期:1999-01-29 00:00:00
abstract::F-box/LRR-repeat protein 2 (FBXL2), a component of Skp-Cullin-F box (SCF) ubiquitin E3 ligase, has been shown to inhibit tumorigenesis by targeting and ubiquitinating several oncoproteins. However, its role in gastric cancer remains poorly understood. Here, by tandem mass spectrometry, we show that FBXL2 interacts wit...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1002/1873-3468.12071
更新日期:2016-02-01 00:00:00
abstract::From human PBMC stimulated with PHA, we have isolated cDNA clones encoding a novel cytokine named SCM-1, which is significantly related to the CC and the CXC chemokines but has only the 2nd and the 4th of the four cysteines conserved in these proteins. Its gene is also distinctly mapped to human chromosome 1. SCM-1 is...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)00093-o
更新日期:1995-02-27 00:00:00
abstract::Vacuolar membrane H(+)-translocating pyrophosphatase (H(+)-PPase) was purified from pumpkin seedlings. Its enzymatic properties including molecular size of constituting polypeptide (75 kDa) were very similar to those of mung bean H(+)-PPase [(1989) J. Biol. Chem. 264, 20068-20073]. The native, functional molecular siz...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)81254-6
更新日期:1991-09-23 00:00:00
abstract::To identify suitable promoters for expressing exogenous genes in arthritic joints, the constitutive, simian virus 40 (SV 40) and IL-1 or metal inducible, human stromelysin and metallothionein (MT) gene promoters were tested for their activity in chondrocytes and synovial fibroblasts. Transient transfection with plasmi...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)01037-x
更新日期:1996-10-21 00:00:00
abstract::Crystallographic analysis of a mutated form of "loopful" GH19 chitinase from rye seeds a double mutant RSC-c, in which Glu67 and Trp72 are mutated to glutamine and alanine, respectively, (RSC-c-E67Q/W72A) in complex with chitin tetrasaccharide (GlcNAc)₄ revealed that the entire substrate-binding cleft was completely o...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2013.07.008
更新日期:2013-08-19 00:00:00
abstract::Though phospholipase C PLCgamma2 is known to play an important role in platelet activation by collagen and fibrinogen, its importance in GPIb-mediated platelet activation is less well understood. To better understand the role of PLCgamma2 in GPIb-mediated adhesion and thrombus formation, we examined the ability of wil...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2004.07.048
更新日期:2004-08-27 00:00:00
abstract::Endoplasmic reticulum stress (ER-stress) is associated with ataxia telangiectasia mutated (ATM) gene. We present here conclusive data showing that ATM blocks ER-stress induced by tunicamycin or ionizing radiation (IR). X-box protein-1 (XBP-1) splicing, GRP78 expression and caspase-12 activation were increased by tunic...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2009.02.002
更新日期:2009-03-04 00:00:00
abstract::AQP3 is a water/glycerol transporter expressed at the basolateral membrane of colonic epithelial cells. Although AQPs are expressed in the gastrointestinal tract, their effect on intestinal barrier has not been clear. Here, we showed that knockdown of AQP3 caused a dramatic, dose-dependent increase in E. coli C25 tran...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2011.08.045
更新日期:2011-10-03 00:00:00
abstract::We have previously described a partial cDNA sequence encoding a RhoGAP protein, GAP25 that is homologous to the recently reported ArhGAP9 and ArhGAP12. We now describe a related new member ArhGAP15 that shares a number of domain similarities, including a pleckstrin homology (PH) domain, a RhoGAP domain and a novel mot...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(03)00213-8
更新日期:2003-03-27 00:00:00
abstract::Fructose-1,6-bisphosphatase, a key enzyme in gluconeogenesis, undergoes catabolite inactivation when glucose is added to gluconeogenetically active cells of the yeast Saccharomyces cerevisiae. Phosphorylation of the enzyme is followed by rapid degradation. To elucidate the cellular proteolytic system involved in catab...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)00668-7
更新日期:1994-08-01 00:00:00
abstract::Toll-like receptors (TLRs) are crucial components of the innate immune system, coupling pathogen recognition to a cellular response. We used the MAPPIT mammalian two-hybrid technique to investigate protein-protein interactions in the early steps in TLR signalling. A partial TLR-adaptor interaction map was constructed ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2007.01.026
更新日期:2007-02-20 00:00:00
abstract::It is known that chemokine-like factor superfamily 8 (CKLFSF8), a member of the CKLF superfamily, has four putative transmembrane regions and a MARVEL domain. Its structure is similar to TM4SF11 (plasmolipin) and widely distributed in normal tissue. However, its function is not yet known. We show here that CKLFSF8 is ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.10.021
更新日期:2005-11-21 00:00:00
abstract::Treatment of the chemically transformed fibroblasts BP-A31 and other cell lines with low concentrations of cycloheximide (CHM) for 72 h followed by the removal of the protein synthesis inhibitor leads to the proliferation of alpha-difluoromethylornithine (DFMO)-resistant phenotypes. These drug-resistant cells contain ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)81349-7
更新日期:1986-09-29 00:00:00