Abstract:
:AQP3 is a water/glycerol transporter expressed at the basolateral membrane of colonic epithelial cells. Although AQPs are expressed in the gastrointestinal tract, their effect on intestinal barrier has not been clear. Here, we showed that knockdown of AQP3 caused a dramatic, dose-dependent increase in E. coli C25 translocation, with the reduction of TEER and increasing LY permeability. Western blots revealed that expression of Claudin-1 and Occludin were significantly decreased in the AQP3 knockdown group, demonstrating that this treatment enhances paracellular permeability via an opening of the tight junction complex. These data not only describe the correlation between transcellular and paracellular pathways in human intestines, but also show that targeted knockdown of AQP3 might impair the intestinal barrier integrity.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Zhang W,Xu Y,Chen Z,Xu Z,Xu Hdoi
10.1016/j.febslet.2011.08.045subject
Has Abstractpub_date
2011-10-03 00:00:00pages
3113-9issue
19eissn
0014-5793issn
1873-3468pii
S0014-5793(11)00658-2journal_volume
585pub_type
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