Abstract:
:The active A1 peptide of cholera toxin was linked by a disulphide bond to the lectin of Wisteria floribunda. The resulting conjugate activated the adenylate cyclase of intact U937 or K562 cells at the same concentrations as native toxin did, but to a greater extent. Activation was inhibited by N-acetyl-D-galactosamine or by antisera to the lectin or peptide. The characteristic lag phase between addition of toxin to cells and activation of cyclase was not found with the conjugate or with free A1 peptide.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
van Heyningen Sdoi
10.1016/0014-5793(83)80035-0subject
Has Abstractpub_date
1983-11-28 00:00:00pages
132-4issue
1eissn
0014-5793issn
1873-3468journal_volume
164pub_type
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