Abstract:
:Histological studies suggest that adrenal medulla chromaffin cells in situ are polarised, but functional evidence is lacking. We present here the first demonstration for polarisation of exocytosis in isolated, spherical, bovine chromaffin cells. Cells were stimulated with 70 mM K(+) to cause a marked enhancement of catecholamine release, monitored amperometrically. FM1-43 and dopamine beta-hydroxylase antibodies provided fluorescence confocal pictures that were 2-3-fold more intense in the bottom of the cells, as compared to equatorial or apex planes. This suggests that the solid phase to which the cell attaches serves as a 'trophic' signal for the polarisation of its secretory apparatus.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Cuchillo-Ibáñez I,Michelena P,Albillos A,García AGdoi
10.1016/s0014-5793(99)01115-1keywords:
subject
Has Abstractpub_date
1999-10-01 00:00:00pages
22-6issue
1eissn
0014-5793issn
1873-3468pii
S0014-5793(99)01115-1journal_volume
459pub_type
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