Abstract:
:The protein translocation apparatus in Escherichia coli has been studied both genetically and biochemically. In vitro protein translocation systems involving everted membrane vesicles or reconstituted proteoliposomes have significantly contributed to biochemical clarification of the structure, mechanism and energetics of the apparatus. It is established that SecA, SecY and SecE are essential components, and play fundamental roles in the translocation reaction, and that both ATP and a proton motive force are required for the translocation. A new membrane factor, SecG, was found to participate in the formation of the apparatus, causing significant enhancement of the activity. SecD was found to play a role in the release of translocated proteins from the outer surface of the cytoplasmic membrane.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Tokuda Hdoi
10.1016/0014-5793(94)00317-3subject
Has Abstractpub_date
1994-06-06 00:00:00pages
65-8issue
1eissn
0014-5793issn
1873-3468pii
0014-5793(94)00317-3journal_volume
346pub_type
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