Abstract:
:We have investigated whether the expression in Arabidopsis thaliana seeds of a transgene (the Phaseolus vulgaris arcelin (arc)5-I gene) could be enhanced by the simultaneous introduction of an antisense gene for an endogenous seed storage protein (2S albumin). Seeds of plants transformed with both the arc5-I gene and a 2S albumin antisense gene contained reduced amounts of 2S albumins and increased arcelin-5 (Arc5) accumulation levels compared to lines harboring the arc5-I gene only. Arc5 production could be enhanced to more than 24% of the total seed protein content, suggesting that antisense technology could be of great utility to favor high expression of transgenes.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Goossens A,Van Montagu M,Angenon Gdoi
10.1016/s0014-5793(99)00943-6keywords:
subject
Has Abstractpub_date
1999-07-30 00:00:00pages
160-4issue
1eissn
0014-5793issn
1873-3468pii
S0014-5793(99)00943-6journal_volume
456pub_type
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