Abstract:
:Transthyretin (TTR) is a circulatory protein which plays an important role in the transport of both thyroid hormone and retinol. Hep G2 cells, a human hepatoma-derived cell line, have been used extensively in studies of protein secretion by liver cells. The original description of this cell line indicated that this line, unlike primary hepatocytes, does not secrete TTR. We now report studies which reexamine the ability of Hep G2 cells to synthesize and secrete TTR. For this purpose, total RNA was isolated from Hep G2 cells grown on both uncoated and collagen-coated plastic plates and was examined for TTR expression by Northern blot analysis. TTR mRNA was found to be present in nearly equal amounts in Hep G2 cells cultured in either condition. When Hep G2 cells were cultured in [35S]methionine-containing medium, the cells were found both to synthesize and to secrete immunoprecipitable [35S]TTR. Hep G2 cells were found, by sensitive and specific radioimmunoassay, to contain 142 +/- 91 ng TTR/10(6) cells and to secrete TTR into the medium at a nearly constant rate for at least 24 h after medium change. Our data demonstrate that Hep G2 cells do synthesize and secrete TTR and suggest that this cell line might be useful for studies of the secretion of TTR.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Blaner WS,Bonifácio MJ,Feldman HD,Piantedosi R,Saraiva MJdoi
10.1016/0014-5793(91)80049-9keywords:
subject
Has Abstractpub_date
1991-08-05 00:00:00pages
193-6issue
1-2eissn
0014-5793issn
1873-3468pii
0014-5793(91)80049-9journal_volume
287pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::Signal transduction involving phosphatidylcholine hydrolysis has been investigated in human neutrophils (PMN) after in situ generation of [3H]alkylacyl-sn-glycero-3-phosphocholine ([3H]alkylacyl-GPC) by cell incubation with [3H]alkylacetyl-GPC. When PMN were stimulated with the chemotactic peptide N-formyl-Met-Leu-Phe...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)81457-7
更新日期:1989-07-17 00:00:00
abstract::MRL mice bearing the lpr (Fas) or gld (Fas ligand) mutation, MRL-Fas(lpr) or MRL-FasL(gld), respectively, develop arthritis similar to rheumatoid arthritis, but C3H and C57BL/6 mice bearing such mutations do not. In MRL-Fas(lpr) mice, agalactosylated oligosaccharides in serum IgG increase significantly in comparison t...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)02974-x
更新日期:2001-10-26 00:00:00
abstract::Hypo-osmotic swelling of human Intestine 407 cells leads to a significant increase of intracellular MAPKAP-kinase 2 activity and Hsp27 phosphorylation. Pre-treatment of the cells with the p38 MAP kinase inhibitor SB-203580 blocks this activation, indicating that the hypotonicity-induced activation of MAPKAP kinase 2 i...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)01028-9
更新日期:1996-10-21 00:00:00
abstract::Calcium channels derived from sarcoplasmic reticulum of frog skeletal muscle were fused with planar lipid bilayers. Fractional open times displayed two types of calcium dependence: (i) blockable channels showed a bell-shaped calcium dependence with an activation constant of 4.5 microM, a Hill coefficient for activatio...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)80341-q
更新日期:1993-10-04 00:00:00
abstract::Coronin proteins are widely expressed among eukaryotic organisms. Most coronins consist of a WD-repeat domain followed by a C-terminal coiled coil. Dictyostelium discoideum expresses a single short coronin coronin A, which has been implicated in both actin modulation and multicellular differentiation. Whether coronin ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1002/1873-3468.13787
更新日期:2020-04-16 00:00:00
abstract::The class A Ca2+ channel alpha 1 subunit (alpha 1A) was expressed in Xenopus oocytes alone or in combination with the beta 1b, beta 2a, beta 3, or beta 4 subunit. Analysis of voltage-dependent activation and inactivation in the presence of 1.8 mM external Ca2+ showed an hyperpolarising shift of both relations when com...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)00704-1
更新日期:1996-08-12 00:00:00
abstract::The aim of this study was to investigate the distribution of a calcium-inhibitable adenylyl cyclase type VI (type VI AC) in the central nervous system using an antiserum directed against the N-terminus of type VI AC. Our results indicate that type VI AC immunoreactivity is generally expressed in many brain regions wit...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)01098-9
更新日期:1998-09-25 00:00:00
abstract::In vertebrate photoreceptors the soluble protein arrestin (45 kDa) is involved in controlling the light dependent activity of receptor proteins such as transducin or the cGMP-phosphodiesterase. Arrestin has further been identified as the retinal-S-antigen which is assumed to cause the autoimmune disease uveitis. In a ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80530-g
更新日期:1991-05-06 00:00:00
abstract::We have developed a high-throughput sequencing approach that enables us to determine terminal telomere sequences from tens of thousands of individual Schizosaccharomyces pombe telomeres. This method provides unprecedented coverage of telomeric sequence complexity in fission yeast. S. pombe telomeres are composed of mo...
journal_title:FEBS letters
pub_type: 信件
doi:10.1002/1873-3468.12444
更新日期:2016-12-01 00:00:00
abstract::We provide first large scale analysis of the peculiarities of surface areas of 5658 dissimilar (below 50% sequence similarity) proteins with known 3D-structures that bind to proteins, DNA or RNAs. We show here that area of the protein surface is highly correlated with the protein length. The size of the interface surf...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2015.08.014
更新日期:2015-09-14 00:00:00
abstract::Melittin, deuteromethylated on each of the four amino groups (Gly-1 N alpha and Lys-7, 21, and 23 N epsilon), was prepared by reductive methylation using deuteroformaldehyde and NaBD3CN. Deuterium NMR spectra were obtained for the modified peptide (D-melittin) bound to phospholipid bilayers and erythrocyte ghosts. D-M...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(87)81041-4
更新日期:1987-06-22 00:00:00
abstract::The mutation of a single leucine residue (L38) to methionine (M) is known experimentally to significantly increase the affinity of the synthetic four-alpha-helix bundle (Aalpha(2))(2) for the anesthetic halothane. We present a molecular dynamics study of the mutant (Aalpha(2)-L38M)(2) peptide, which consists of a dime...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)01792-0
更新日期:2000-07-28 00:00:00
abstract::The number of synthetic UTP analogues containing methyl groups in different positions of the ribose moiety were tested as substrates for T7 RNA polymerase (T7 RNAP). Two of these compounds (containing substituents in the 5' position) were shown to be weak substrates of T7 RNAP. 3'Me-UTP was neither substrate nor inhib...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(96)01400-7
更新日期:1997-01-06 00:00:00
abstract::Internalization of membrane proteins has been studied for more than three decades without solving all the underlying mechanisms. Our knowledge of the clathrin-coated endocytosis is sufficient to understand the basic principles. However, more detailed insight is required to recognize why different proteins enter clathr...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/s0014-5793(02)03187-3
更新日期:2002-10-02 00:00:00
abstract::The tryptophan analog, 5-hydroxytryptophan (5HW), has a significant absorbance between 310-320 nm, which allows it to act as an exclusive fluorescence probe in protein mixtures containing a large number of tryptophan residues. Here for the first time a method is reported for the biosynthetic incorporation of 5HW into ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)81346-n
更新日期:1992-10-05 00:00:00
abstract::We have isolated cDNA molecules encoding a protein with the characteristic sequence elements that are conserved between the catalytic domains of protein kinases. This protein is apparently a serine/threonine kinase and is most closely related to the amino-terminal half of the ribosomal protein S6 kinase II first chara...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)81096-7
更新日期:1990-10-29 00:00:00
abstract::Misfolding of the prion protein yields amyloidogenic isoforms, and it shows exacerbating neuronal damage in neurodegenerative disorders including prion diseases. Pituitary adenylate cyclase-activating polypeptide (PACAP) and vasoactive intestinal peptide (VIP) potently stimulate neuritogenesis and survival of neuronal...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)02886-7
更新日期:2002-07-03 00:00:00
abstract::The Ca(2+)-calmodulin dependent protein phosphatase, calcineurin, is thought to mediate the action of the two immunosuppressants, cyclosporin A (CsA) and FK506. Calcineurin from all species consists of a catalytic A subunit and a regulatory peptide B, which plays an essential role in catalysis. The enzymatic function ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)01365-8
更新日期:1995-01-03 00:00:00
abstract::The glucuronic acid analogue of 1-deoxynojirimycin, 2(S)-carboxy-3(R), 4(R), 5(S)-trihydroxypiperidine, recently isolated from seeds of Baphia racemosa, is a novel specific inhibitor of human liver beta-D-glucuronidase and alpha-L-iduronidase. No other glycosidases are inhibited. The inhibition of beta-D-glucuronidase...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)80911-4
更新日期:1984-10-15 00:00:00
abstract::F-box/LRR-repeat protein 2 (FBXL2), a component of Skp-Cullin-F box (SCF) ubiquitin E3 ligase, has been shown to inhibit tumorigenesis by targeting and ubiquitinating several oncoproteins. However, its role in gastric cancer remains poorly understood. Here, by tandem mass spectrometry, we show that FBXL2 interacts wit...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1002/1873-3468.12071
更新日期:2016-02-01 00:00:00
abstract::CREB binding protein (CBP) is an acetyltransferase that plays an important role in many biological processes. Here, we show that Akt phosphorylates CBP at threonine 1871 and suppresses its acetyltransferase activity by impeding the binding of CBP to histone H3, which results in a decrease in lysine K18 acetylation and...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2013.02.023
更新日期:2013-04-02 00:00:00
abstract::Rat basophilic leukemia cells (RBL-2H3) have previously been shown to contain a single type of voltage-activated channel, namely an inwardly rectifying K+ channel, under normal recording conditions. Thus, RBL-2H3 cells seemed like a logical source of mRNA for the expression cloning of inwardly rectifying K+ channels. ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)81215-t
更新日期:1991-09-23 00:00:00
abstract::The in vivo responses of pyruvate dehydrogenase (PDH) complex to starvation and insulin was assessed in heart, diaphragm and red quadriceps muscle. PDH complex activity was decreased by starvation (3.4-10.2-fold), the magnitude of change depending on muscle type. Insulin increased PDH activity in all muscle types. In ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)80777-x
更新日期:1989-07-03 00:00:00
abstract::Here, we report the volume and enthalpy changes accompanying CO photodissociation from the mixed valence form of cytochrome bo3 oxidase from Escherichia coli. The results of photoacoustic calorimetry indicate two kinetic phases with distinct volume and enthalpy changes accompanying CO photodissociation from heme o3 an...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.04.055
更新日期:2005-06-06 00:00:00
abstract::Photosynthetic machinery requires balanced distribution of excitation energy from the light-harvesting complexes to photosystems. The efficiency of light-harvesting is regulated by thermal dissipation of excess energy, while the distribution of energy between photosystems is dependent on STN7 kinase and phosphorylatio...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2012.07.035
更新日期:2012-08-31 00:00:00
abstract::The cytochrome P-450 content of rat hepatocytes declined rapidly over 72 h in culture, due primarily to denaturation to cytochrome P-420. Six different media were investigated for their ability to conserve cytochrome P-450 during culture, and the most successful was a modified Earle's medium. After 72 h culture in thi...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(85)80436-1
更新日期:1985-10-07 00:00:00
abstract::The mechanism of the maize pyruvate, phosphate dikinase-catalyzed conversion of adenosine 5'-triphosphate, orthophosphate and pyruvate to adenosine 5'-monophosphate, inorganic pyrophosphate and phosphoenolpyruvate, respectively, was determined by using transient kinetic techniques. The data obtained demonstrate that c...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)81358-u
更新日期:1990-11-12 00:00:00
abstract::A rat urokinase receptor (uPAR) cDNA fragment was amplified by RT-PCR from RNA of the rat osteoblastic cell line CFK-1. Using this DNA species as a hybridization probe two rat uPAR cDNAs were isolated from a CFK-1 cDNA library. These two cDNAs encode an identical uPAR protein except for a single base mutation which re...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)80118-5
更新日期:1994-01-24 00:00:00
abstract::Irreversible conversion of the purified zinc-containing 7Fe ferredoxin from the thermoacidophilic archaeon Sulfolobus sp. strain 7 was found to occur under aerobic conditions at pH 5.0 and at 4 degrees C. This process accompanied a substantial increase of the electron paramagnetic resonance signal attributed to a [3Fe...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)01286-6
更新日期:1997-11-10 00:00:00
abstract::Incubation of cholinergic synaptic vesicles purified from the electric organ of Torpedo ocellata with radiolabeled [D-Ala2, D-Leu5]-enkephalin results in its accumulation in the vesicles. Kinetic analysis of the initial rates of uptake reveals temperature-dependent saturation kinetics which are best fitted by a single...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(85)80946-7
更新日期:1985-04-08 00:00:00