Abstract:
:To study Bax-induced release of cytochrome c in vivo, we have expressed a cytochrome c-GFP (green fluorescent protein) fusion in Saccharomyces cerevisiae cells null for the expression of the endogenous cytochrome. We show here that cytochrome c-GFP is efficiently localised to mitochondria and able to function as an electron carrier between complexes III and IV of the respiratory chain. Strikingly, while natural cytochrome c is released into the cytoplasm upon expression of Bax, the cytochrome c-GFP fusion is not. Nevertheless, cells co-expressing Bax and the cytochrome c-GFP fusion die, indicating that mitochondrial release of cytochrome c is not essential for cell death to occur in yeast. The failure to release cytochrome c-GFP is presumed to arise from increased bulk due to the GFP moiety. We propose that in intact yeast cells, Bax-induced release of cytochrome c into the cytoplasm occurs through a selective pore and not as a consequence of the non-specific breakage of the mitochondrial outer membrane.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Roucou X,Prescott M,Devenish RJ,Nagley Pdoi
10.1016/s0014-5793(00)01404-6keywords:
subject
Has Abstractpub_date
2000-04-14 00:00:00pages
235-9issue
2-3eissn
0014-5793issn
1873-3468pii
S0014-5793(00)01404-6journal_volume
471pub_type
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