Modulation of gastric H+,K+-transporting ATPase function by sodium.

abstract：:WT1 encodes a zinc finger protein with a key role in urogenital development that is inactivated in a subset of Wilms' tumors. This tumor suppressor gene product contains an amino-terminal dimerization domain required for trans-inhibition of wild-type WT1 activity by mutants defective for DNA binding. In the course of ...

journal_title：FEBS letters

authors： Bruening W,Moffett P,Chia S,Heinrich G,Pelletier J

更新日期：1996-09-09 00:00:00

abstract：:In HL-60 cells signal transduction via sphingomyelin hydrolysis (sphingomyelin cycle) is induced by binding of tumor necrosis factor alpha (TNFalpha) to cell surface TNFalpha receptor. We found that IgG immunoglobulins activate sphingomyelin hydrolysis in plasma membrane of HL-60 cells, with kinetics similar to that o...

journal_title：FEBS letters

authors： Glick D,Barenholz Y

更新日期：1996-10-07 00:00:00

abstract：:Aminoacyl-tRNA synthetases, a group of enzymes catalyzing aminoacyl-tRNA formation, may possess inherent editing activity to clear mistakes arising through the selection of non-cognate amino acid. It is generally assumed that both editing substrates, non-cognate aminoacyl-adenylate and misacylated tRNA, are hydrolyzed...

journal_title：FEBS letters

authors： Gruic-Sovulj I,Rokov-Plavec J,Weygand-Durasevic I

更新日期：2007-10-30 00:00:00

abstract：:Circular dichroism (CD) spectra have been obtained from several variants of green fluorescent protein: blue fluorescent protein (BFP), enhanced cyan fluorescent protein (CFP), enhanced green fluorescent protein (GFP), enhanced yellow fluorescent protein (YFP), all from Aequorea victoria, and the red fluorescent protei...

journal_title：FEBS letters

authors： Visser NV,Hink MA,Borst JW,van der Krogt GN,Visser AJ

更新日期：2002-06-19 00:00:00

abstract：:The actin-depolymerising factor (ADF)/cofilin group of proteins are stimulus-responsive actin-severing proteins, members of which are regulated by reversible phosphorylation. The phosphorylation site on the maize ADF, ZmADF3, is Ser-6 but the kinase responsible is unknown [Smertenko et al., Plant J. 14 (1998) 187-193]...

journal_title：FEBS letters

authors： Allwood EG,Smertenko AP,Hussey PJ

更新日期：2001-06-15 00:00:00

abstract：:A serum-free medium conditioned by U937, a human cell line of monocyte/macrophage origin, was found capable of inducing apoptosis on exponentially growing U937 cells themselves (autocrine suicide). The apoptosis-inducing agent is a macromolecule and possibly a protein (SKT factor), with a relative molecular mass in th...

journal_title：FEBS letters

authors： Ghibelli L,Coppola S,Nosseri C,Bergamini A,Beninati S

更新日期：1994-05-09 00:00:00

abstract：:Yeast Golgi ecto-ATPase Ynd1p is an unusual type III membrane protein with the longest translocated N-terminus reported. Sequential deletion analysis reveals that translocation of this 500-residue-long hydrophilic domain across the membranes requires the C-terminal transmembrane domain of Ynd1p and its flanking region...

journal_title：FEBS letters

authors： Zhong X,Malhotra R,Guidotti G

更新日期：2005-10-24 00:00:00

abstract：:Induction of a microsomal oleate delta12 (n-6) desaturase which is mainly responsible for an increase in membrane lipid unsaturation at low temperature has been observed in the free-living amoeba Acanthamoeba castellanii. In this study we show that the enzyme can also be regulated by oxygen independently of temperatur...

journal_title：FEBS letters

authors： Thomas K,Rutter A,Suller M,Harwood J,Lloyd D

更新日期：1998-03-20 00:00:00

abstract：:Oligonucleotide probes based on the known amino acid sequence of salmon calcitonin were used to screen a cDNA library obtained from ultimobranchial glands of salmon for clones encoding salmon calcitonin. From the cDNA sequence of strongly hybridizing clones the complete primary structure of the calcitonin precursor co...

journal_title：FEBS letters

authors： Pöschl E,Lindley I,Hofer E,Seifert JM,Brunowsky W,Besemer J

更新日期：1987-12-21 00:00:00

abstract：:14-3-3 proteins were the first signaling molecules to be identified as discrete phosphoserine/threonine binding modules. This family of proteins, which includes seven isotypes in human cells and up to 15 in plants, plays critical roles in cell signaling events that control progress through the cell cycle, transcriptio...

journal_title：FEBS letters

authors： Yaffe MB

更新日期：2002-02-20 00:00:00

abstract：:The β-xylosidase B from Bifidobacterium adolescentis ATCC15703 belongs to the newly characterized family 120 of glycoside hydrolases. In order to investigate its catalytic mechanism, an extensive kinetic study of the wild-type enzyme and mutants targeting the three highly conserved residues Asp(393), Glu(416) and Glu(...

journal_title：FEBS letters

authors： Cecchini DA,Fauré R,Laville E,Potocki-Veronese G

更新日期：2015-10-07 00:00:00

abstract：:Cyclic AMP (cAMP) and its action by way of cAMP-dependent protein kinase is important for sperm motility. Previous studies on germ cells have demonstrated a selective decrease in the amount of type I cAMP-dependent protein kinase during spermatid development, and that type II was the major form present in elongating s...

journal_title：FEBS letters

authors： Oyen O,Scott JD,Cadd GG,McKnight GS,Krebs EG,Hansson V,Jahnsen T

更新日期：1988-03-14 00:00:00

abstract：:Translocation of folded proteins by the Tat system of Escherichia coli is believed to rely on the presence of phosphatidylethanolamine (PE) and the negatively charged phospholipids cardiolipin (CL) and phosphatidylglycerol (PG). Here, we show that while PE is indeed essential for activity, the Tat system is fully func...

journal_title：FEBS letters

authors： Rathmann C,Schlösser AS,Schiller J,Bogdanov M,Brüser T

更新日期：2017-09-01 00:00:00

abstract：:The aim of this study was to evaluate the involvement of oxidative stress in glutamate-evoked transglutaminase (TGase) upregulation in astrocyte cultures (14 DIV). A 24 h exposure to glutamate caused a dose-dependent depletion of glutathione intracellular content and increased the ROS production in cell cultures. Thes...

journal_title：FEBS letters

authors： Campisi A,Caccamo D,Li Volti G,Currò M,Parisi G,Avola R,Vanella A,Ientile R

更新日期：2004-12-03 00:00:00

abstract：:Tumour necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) is a member of the TNF family of cytokines that promotes apoptosis and NF-kappaB activation. Here we show that recombinant hu-TRAIL initiates the activation of multiple caspases, the loss of mitochondrial transmembrane potential, the cleavage of BI...

journal_title：FEBS letters

authors： Keogh SA,Walczak H,Bouchier-Hayes L,Martin SJ

更新日期：2000-04-07 00:00:00

abstract：:Mechanisms involved in cell volume regulation are important in SS, SC cells as they might be involved in determining the extent of sickling and the generation of dense cells and irreversibly sickled cells. We have studied in these cells the response to cell swelling of the K+,Cl- transporter. We found that Hb SS, SC a...

journal_title：FEBS letters

authors： Canessa M,Spalvins A,Nagel RL

更新日期：1986-05-05 00:00:00

abstract：:Ca2+ release from sarcoplasmic reticulum membranes, activated by alkaline pH occurs only when EGTA is present in the release medium. Addition of very low concentrations of Ca2+ to the medium inhibits Ca2+ release. The concentration of free Ca2+ required for 50% inhibition ranges from between 5 and 20 nM in different e...

journal_title：FEBS letters

authors： Argaman A,Shoshan-Barmatz V

更新日期：1989-01-16 00:00:00

abstract：:In a subset of olfactory epithelium the odorant receptor guanylate cyclase, ONE-GC, is a central transduction component of the cyclic GMP signaling pathway. The odorant binds to the extracellular domain and activates its intracellular catalytic domain to generate the odorant second messenger, cyclic GMP. The present s...

journal_title：FEBS letters

authors： Duda T,Sharma RK

更新日期：2009-04-17 00:00:00

abstract：:In this study we identify the molecules involved in the MAPK signal transduction pathway (Ras, Raf-1, Mek, Mek-P and MAPK) in highly purified endosomal fractions isolated from rat liver. Biochemical analysis shows that only the early-sorting endocytic compartment contains activated Raf-1 and Mek. Finally, the exogenou...

journal_title：FEBS letters

authors： Pol A,Calvo M,Enrich C

更新日期：1998-12-11 00:00:00

abstract：:The replacement of O2 with CO was studied on cobalt-iron hemoglobin hybrids. Both proto- and mesocobalt hemes were used for the reconstitution. In the oxy quaternary conformation no difference is observed between alpha- and beta-subunits when only proto hemes are present in the hybrid (k4 = 30 s-1, k'4/l'4 = 2.5). If ...

journal_title：FEBS letters

authors： Oertle M,Winterhalter KH,Di Iorio EE

更新日期：1983-03-07 00:00:00

abstract：:A new 175-kDa membrane protein was isolated from chicken gizzard smooth muscle. Antibodies to 175-kDa protein were used for localization of this protein in smooth and cardiac muscles. In both types of muscle 175-kDa protein was localized near plasma membrane. 175-kDa protein was able to interact specifically with vinc...

journal_title：FEBS letters

authors： Koteliansky VE,Gneushev GN,Belkin AM

更新日期：1985-03-11 00:00:00

abstract：:The unfolded protein response (UPR)--the endoplasmic reticulum stress response--is found in various pathologies including ischemia-reperfusion injury (IRI). However, its role during IRI is still unclear. Here, by combining two different bioinformatical methods--a method based on ordinary differential equations (Time S...

journal_title：FEBS letters

authors： Le Pape S,Dimitrova E,Hannaert P,Konovalov A,Volmer R,Ron D,Thuillier R,Hauet T

更新日期：2014-08-25 00:00:00

abstract：:Using acridine orange as a pH gradient probe, the effects of valinomycin and FCCP on the pH gradient across lysosomal membranes in an ATP-free medium as well as on the rate of the inward ATP-driven proton translocation were investigated. Both lysosome-enriched and highly purified lysosomal preparations from rat liver ...

journal_title：FEBS letters

authors： Dell'Antone P

更新日期：1984-03-12 00:00:00

abstract：:Mitochondrial translocation of ceramides triggers Bax-dependent apoptosis. To elucidate how ceramides activate Bax and commit cells to death, we developed a switchable version of the ceramide transfer protein CERT, sCERT. Upon its drug-induced recruitment to mitochondria, sCERT retains the ability to bind VAP proteins...

journal_title：FEBS letters

authors： Jain A,Dadsena S,Holthuis JCM

更新日期：2020-11-01 00:00:00

abstract：:We report a rapid and sensitive colorimetric approach to quantitate the amount of glucose transporters exposed at the surface of intact cells, using L6 muscle cells expressing GLUT4 containing an exofacial myc epitope. Unstimulated cells exposed to the surface 5 fmol GLUT4myc per mg protein. This value increased to 10...

journal_title：FEBS letters

authors： Wang Q,Khayat Z,Kishi K,Ebina Y,Klip A

更新日期：1998-05-08 00:00:00

abstract：:Ovine trophoblastic protein B (oTPB), an embryonic protein, is a 20 kDa secretory protein which is synthesized by the ovine conceptus from days 12 to 22 of pregnancy. oTPB was purified by HPLC using ion-exchange chromatography on a DEAE column and was subsequently chromatographed on a reversed-phase column. Automated ...

journal_title：FEBS letters

authors： Charpigny G,Reinaud P,Huet JC,Guillomot M,Charlier M,Pernollet JC,Martal J

更新日期：1988-02-08 00:00:00

abstract：:Affinity labeling studies have identified several conserved tyrosine residues in the alpha subunit of the nicotinic acetylcholine receptor (alpha Y93, alpha Y190, and alpha Y198) as being in or near the ligand binding site. Mutagenesis studies from several laboratories have shown that substitution of phenylalanine for...

journal_title：FEBS letters

authors： Aylwin ML,White MM

更新日期：1994-07-25 00:00:00

abstract：:Co-expression of the two genes encoding the alpha- and beta-subunit of the Thermoplasma acidophilum thermosome in Escherichia coli yielded fully assembled hetero-oligomeric complexes (alpha+beta). Surprisingly, also separate expression of both genes resulted in formation of hexadecameric complexes (alpha, beta) in the...

journal_title：FEBS letters

authors： Waldmann T,Nitsch M,Klumpp M,Baumeister W

更新日期：1995-11-27 00:00:00

abstract：:The crystal structure of Arthromyces ramosus peroxidase (ARP) in complex with benzhydroxamic acid (BHA) as determined by X-ray analysis at 1.6 A shows unambiguously how BHA binds to ARP. BHA is located in the distal heme pocket. Its functional groups are held by three hydrogen bonds to His56N(epsilon), Arg52N(epsilon)...

journal_title：FEBS letters

authors： Itakura H,Oda Y,Fukuyama K

更新日期：1997-07-21 00:00:00

abstract：:Internalization of membrane proteins has been studied for more than three decades without solving all the underlying mechanisms. Our knowledge of the clathrin-coated endocytosis is sufficient to understand the basic principles. However, more detailed insight is required to recognize why different proteins enter clathr...

journal_title：FEBS letters

authors： Strous GJ,Gent J

更新日期：2002-10-02 00:00:00