Abstract:
:Using acridine orange as a pH gradient probe, the effects of valinomycin and FCCP on the pH gradient across lysosomal membranes in an ATP-free medium as well as on the rate of the inward ATP-driven proton translocation were investigated. Both lysosome-enriched and highly purified lysosomal preparations from rat liver were used with identical results. Ionophore effects were found to be different depending upon whether passive ion fluxes or ATP-driven H+ translocation were involved and supported the existence of a membrane potential in the latter case. Anions stimulated the rate of ATP-driven proton translocation and stimulation increased with increasing anion lipophilicity. These results strongly support the electrogenic nature of the lysosomal proton pump.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Dell'Antone Pdoi
10.1016/0014-5793(84)80198-2subject
Has Abstractpub_date
1984-03-12 00:00:00pages
15-22issue
1eissn
0014-5793issn
1873-3468journal_volume
168pub_type
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