Localization of calponin binding sites in the structure of 90 kDa heat shock protein (Hsp90).

abstract：:The effect of angiotensin II (Ang II) on the early growth response gene-1 (Egr-1) mRNA, on the Egr-1 protein and on the phosphoinositide PI turnover signalling system was investigated in the presence and absence of EXP3174, a potent non-peptide Ang II receptor antagonist. Ang II induced an accumulation of 3.4 kb Egr-1...

journal_title：FEBS letters

authors： Sachinidis A,Weisser P,Ko Y,Schulte K,Meyer zu Brickwedde K,Neyses L,Vetter H

更新日期：1992-11-23 00:00:00

abstract：:Interleukin-22 (IL-22) plays an important role in the regulation of immune and inflammatory responses in mammals. The IL-22 binding protein (IL-22BP), a soluble receptor that specifically binds IL-22, prevents the IL-22/interleukin-22 receptor 1 (IL-22R1)/interleukin-10 receptor 2 (IL-10R2) complex assembly and blocks...

journal_title：FEBS letters

authors： de Moura PR,Watanabe L,Bleicher L,Colau D,Dumoutier L,Lemaire MM,Renauld JC,Polikarpov I

更新日期：2009-04-02 00:00:00

abstract：:Malonyl-CoA-acyl carrier protein transacylase (MCAT) transfers the malonyl group from malonyl-CoA to holo-acyl carrier protein (ACP), and since malonyl-ACP is a key building block for fatty-acid biosynthesis it is considered as a promising antibacterial target. The crystal structures of MCAT from Staphylococcus aureus...

journal_title：FEBS letters

authors： Hong SK,Kim KH,Park JK,Jeong KW,Kim Y,Kim EE

更新日期：2010-03-19 00:00:00

abstract：:Glycosylphosphatidylinositol (GPI) is synthesized in the endoplasmic reticulum (ER) and added onto proteins to form GPI-anchored proteins. Among the many proteins involved in this process, ACAT-related enzyme-2 required for viability 1 (Arv1) is a candidate, functioning as a flippase that translocates GPI intermediate...

journal_title：FEBS letters

authors： Okai H,Ikema R,Nakamura H,Kato M,Araki M,Mizuno A,Ikeda A,Renbaum P,Segel R,Funato K

更新日期：2020-08-01 00:00:00

abstract：:We report on the cDNA cloning and characterization of a novel short-chain isoprenyl diphosphate synthase from the aphid Myzus persicae. Of the three IPPS cDNAs we cloned, two yielded prenyltransferase activity following expression in Escherichia coli; these cDNAs encode identical proteins except for the presence, in o...

journal_title：FEBS letters

authors： Vandermoten S,Charloteaux B,Santini S,Sen SE,Béliveau C,Vandenbol M,Francis F,Brasseur R,Cusson M,Haubruge E

更新日期：2008-06-11 00:00:00

abstract：:We have purified two growth factors from dogfish brain and retina by using their binding affinity for heparin-Sepharose. These growth factors were eluted at 1 and 2 M NaCl similarly to those purified from bovine brain or retina. Their mitogenic activity was assayed in vitro on the same mammalian cells: bovine lens epi...

journal_title：FEBS letters

authors： Lagente O,Diry M,Courtois Y

更新日期：1986-07-07 00:00:00

abstract：:The influence of islet-activating protein (IAP), a Bordetella pertussis toxin, was studied on adenylate cyclase and GTPase activities in rat adipocyte membranes. Pretreatment of rats or intact rat adipocytes with IAP did not affect adenylate cyclase inhibition by the stable GTP analog, GTP gamma S, whereas inhibition ...

journal_title：FEBS letters

authors： Aktories K,Schultz G,Jakobs KH

更新日期：1983-05-30 00:00:00

abstract：:Over 400 human proteases documented in secondary databases can already be delineated in genomic sequence. A Genome Ontology annotation of 30585 sequences in the provisional human proteome set recognises 498 proteases, i.e. 1.6%. Homology searches against finished sequence and comparisons between mouse and zebrafish ar...

journal_title：FEBS letters

authors： Southan C

更新日期：2001-06-08 00:00:00

abstract：:Southern blotting using a 5'-proximal probe of the Saccharomyces cerevisiae CYR1 gene has revealed heterogeneity in laboratory strains. It is demonstrated that strain AB320 contains a Ty1 element inserted in the promoter region of CYR1. The Ty1 orientation suggests that transcription of CYR1 is initiated downstream fr...

journal_title：FEBS letters

authors： Lenzen G,Masson P,Jacquemin JM,Danchin A

更新日期：1987-07-13 00:00:00

abstract：:Quantitative structure activity relationships (QSARs) for the conversion of nitrobenzimidazolones and nitrobenzimidazoles by rat liver DT-diaphorase (EC 1.6.99.2) are described. The parameter used for description of the QSARs is the energy of the lowest unoccupied molecular orbital (E(LUMO)) of the nitroaromatic compo...

journal_title：FEBS letters

authors： Anusevicius Z,Soffers AE,Cénas N,Sarlauskas J,Segura-Aguilar J,Rietjens IM

更新日期：1998-05-15 00:00:00

abstract：:The thermophilic cyanobacterial strain, PCC7518, originally identified as 'Mastigocladus laminosus HTF' does not show branchings or heterocysts. The absence of branchings supports the later assignment to the genus Chlorogloeopsis. The absence of heterocysts may be the result of a mutation because heterocysts were obse...

journal_title：FEBS letters

authors： Wilmotte A,Van der Auwera G,De Wachter R

更新日期：1993-02-08 00:00:00

abstract：:The cellular uptake of a model antisense oligonucleotide complementary to 21 bases of the bovine GLUT-1 glucose transporter mRNA and a model vasopressin peptide that were biotinylated, was markedly stimulated by the presence of avidin, a cationic protein. Conversely, the bacteria homologue of avidin, streptavidin, whi...

journal_title：FEBS letters

authors： Pardridge WM,Boado RJ

更新日期：1991-08-19 00:00:00

abstract：:Siamycin I disrupts growth and quorum sensing in Enterococcus faecalis. Using purified intact protein, we demonstrate here that quorum membrane sensor kinase FsrC is a direct target of siamycin I, reducing pheromone-stimulated autophosphorylation activity by up to 91%. Inhibition was non-competitive with ATP as substr...

journal_title：FEBS letters

authors： Ma P,Nishiguchi K,Yuille HM,Davis LM,Nakayama J,Phillips-Jones MK

更新日期：2011-09-02 00:00:00

abstract：:Saccharomyces cerevisiae mutant strain DTY26, lacking ACE1, the protein mediator for the induction of metallothionein gene expression, is unable to increase Cu,Zn superoxide dismutase mRNA in response to copper. In the wild-type strain DTY22 transcription of both Cu,Zn superoxide dismutase and metallothionein genes is...

journal_title：FEBS letters

authors： Carri MT,Galiazzo F,Ciriolo MR,Rotilio G

更新日期：1991-01-28 00:00:00

abstract：:A human vesicular monoamine transporter cDNA has been identified by screening a human brainstem library using sequences from the rat brain synaptic vesicle monoamine transporter (SVMT) [(1992) Cell 70, 539-551; (1992) Proc. Natl. Acad. Sci. USA 89, 10993-10997]. The hSVMT shares 92% amino acid identity with the rat se...

journal_title：FEBS letters

authors： Surratt CK,Persico AM,Yang XD,Edgar SR,Bird GS,Hawkins AL,Griffin CA,Li X,Jabs EW,Uhl GR

更新日期：1993-03-08 00:00:00

abstract：:Covalent dimerization of the adrenocorticotropin fragment ACTH11-24 increases its antagonistic potency on the ACTH-induced steroidogenesis in isolated bovine fasciculata/reticularis cells by 3 orders of magnitude when the C-termini are linked via a 10 A long spacer. This strong potentiation, probably mediated by cross...

journal_title：FEBS letters

authors： Fauchère JL,Rossier M,Capponi A,Vallotton MB

更新日期：1985-04-22 00:00:00

abstract：:Elongation factor EF-Tu from Escherichia coli was labelled with N-[14C]tosyl-L-phenylalanylchloromethane, digested with trypsin and the peptides obtained separated by HPLC. The only radioactive peak recovered corresponded to tryptic peptide containing residues 75-98. Sequencing of the peptide by automated Edman degrad...

journal_title：FEBS letters

authors： Jonák J,Petersen TE,Clark BF,Rychlík I

更新日期：1982-12-27 00:00:00

abstract：:We examined the expression of peroxisome proliferator-activated receptors (PPARs) and the role of PPARs in cytokine production in mouse bone marrow-derived mast cells (mBMMCs). mBMMCs expressed PPARbeta strongly and gamma slightly, but not alpha. Activation of mBMMCs with antigen or calcium ionophore resulted in the i...

journal_title：FEBS letters

authors： Sugiyama H,Nonaka T,Kishimoto T,Komoriya K,Tsuji K,Nakahata T

更新日期：2000-02-11 00:00:00

abstract：:Heme is essential and synthesized via highly regulated processes. For this reason, most organisms strive to recycle it or acquire it from their environment. When heme is bound to proteins noncovalently, degradation of the polypeptide is sufficient to release it. However, in some hemoproteins, such as c-type cytochrome...

journal_title：FEBS letters

authors： Murphey AC,Mavridou DAI,Hodgkin J,Ferguson SJ

更新日期：2018-03-01 00:00:00

abstract：:HBV particles with genome derived from spliced mRNAs accumulate in patients with virus-derived severe liver necrosis and fibrosis. We investigated the role of an intronic element (intronic splicing silencer-long, ISS(L)) on splicing of HBV minigene transcripts. Removal of the entire ISS(L) showed two-fold increase in ...

journal_title：FEBS letters

authors： Chowdhury JB,Roy D,Ghosh S

更新日期：2011-10-20 00:00:00

abstract：:The maturation of Borna disease virus (BDV) glycoprotein GP was studied in regard to intracellular compartmentalization, compartmentalization signal-domains, proteolytic processing, and packaging into virus particles. Our data show that BDV-GP is (i) predominantly located in the endoplasmic reticulum (ER), (ii) partia...

journal_title：FEBS letters

authors： Eickmann M,Kiermayer S,Kraus I,Gössl M,Richt JA,Garten W

更新日期：2005-08-29 00:00:00

abstract：:The ability of three fatty acids and their respective ethyl esters, to promote generation of reactive oxygen species (ROS), was compared in a preparation of rat brain synaptosomes. Arachidonic but not palmitic or linoleic acids promoted ROS generation. Ethyl esterification of each fatty acid significantly enhanced ROS...

journal_title：FEBS letters

authors： Bondy SC,Marwah S

更新日期：1995-11-13 00:00:00

abstract：:The folding of HIV gp41 into a 6-helix bundle drives virus-cell membrane fusion. To examine the structural relationship between the 6-helix bundle core domain and other regions of gp41, we expressed in Escherichia coli, the entire ectodomain of HIV-2(ST) gp41 as a soluble, trimeric maltose-binding protein (MBP)/gp41 c...

journal_title：FEBS letters

authors： Lay CS,Wilson KA,Kobe B,Kemp BE,Drummer HE,Poumbourios P

更新日期：2004-06-04 00:00:00

abstract：:In a previous investigation of the rDNA region in Tetrahymena pyriformis mitochondrial DNA, we identified a putative tRNA(Met) gene [Heinonen et al. (1987) J. Biol. Chem. 262, 2879-2887]. On the basis of Northern hybridization analyses, we suggested that this gene is expressed, even though the resulting tRNA would be ...

journal_title：FEBS letters

authors： Schnare MN,Greenwood SJ,Gray MW

更新日期：1995-03-27 00:00:00

abstract：:CpG dinucleotides are efficiently methylated in vertebrate genomes except in the CpG islands having a high C+G content. Methylated CpGs are the single most mutated dinucleotide. Sequences surrounding disease causing CpG mutation sites were analyzed from locus-specific mutation databases. Both tetra- and heptanucleotid...

journal_title：FEBS letters

authors： Ollila J,Lappalainen I,Vihinen M

更新日期：1996-11-04 00:00:00

abstract：:cDNA for a novel proton/organic cation transporter, OCTN1, was cloned from human fetal liver and its transport activity was investigated. OCTN1 encodes a 551-amino acid protein with 11 transmembrane domains and one nucleotide binding site motif. It is strongly expressed in kidney, trachea, bone marrow and fetal liver ...

journal_title：FEBS letters

authors： Tamai I,Yabuuchi H,Nezu J,Sai Y,Oku A,Shimane M,Tsuji A

更新日期：1997-12-08 00:00:00

abstract：:A cytosolic calmodulin wave has been described in the pre-replicative phase of rat liver regeneration. Here we demonstrate that this calmodulin increase is reduced by injection of cycloheximide (translation inhibitor) and actinomycin D (transcription inhibitor). In addition, we found that calmodulin mRNA levels are in...

journal_title：FEBS letters

authors： Piõl MR,Berchtold MW,Bachs O,Heizmann CW

更新日期：1988-04-25 00:00:00

abstract：:Trypsin treatment of HeLa cells results in a limited proteolysis of the coxsackievirus and adenovirus receptor (CAR) after which the cleaved CAR remains cell-associated and tryptic peptides remain associated through disulfide bonds. Trypsin-treated HeLa cells remain susceptible to infection with coxsackievirus B and p...

journal_title：FEBS letters

authors： Carson SD

更新日期：2000-11-03 00:00:00

abstract：:Acrosin inhibitors of seminal vesicle origin, after binding to their acceptor molecules on the anterior part of ejaculated sperm, are thought to be important capacitation factors, protecting zona binding sites during sperm uterine passage, and then dissociating to allow sperm binding to the zona pellucida of the oocyt...

journal_title：FEBS letters

authors： Jonáková V,Calvete JJ,Mann K,Schäfer W,Schmid ER,Töpfer-Petersen E

更新日期：1992-02-03 00:00:00

abstract：:It has recently been discovered that glutathione-dependent formaldehyde dehydrogenase (FALDH) exhibits a strong S-nitrosoglutathione reductase activity. Plants use NO and S-nitrosothiols as signaling molecules to activate defense mechanisms. Therefore, it is interesting to investigate the regulation of FALDH by mechan...

journal_title：FEBS letters

authors： Díaz M,Achkor H,Titarenko E,Martínez MC

更新日期：2003-05-22 00:00:00