N-Tosyl-L-phenylalanylchloromethane reacts with cysteine 81 in the molecule of elongation factor Tu from Escherichia coli.

abstract：:Sulfated glycoprotein 2 (SGP-2) mRNA progressively increased in the ventral prostate of the aging rat, reaching, at 24 months, 4-fold higher than at 3 months. Ornithine decarboxylase (ODC) mRNA peaked at 6 months (4-fold increase), and at 12 and 24 months was maintained at higher levels than at 3 months. ODC enzymatic...

journal_title：FEBS letters

authors： Bettuzzi S,Strocchi P,Marinelli M,Astancolle S,Davalli P,Corti A

更新日期：1994-07-18 00:00:00

abstract：:Coenzyme A ligases play an important role in metabolism by catalyzing the activation of carboxylic acids. In this study we describe the synthesis of aminoacyl-coenzyme As (CoAs) catalyzed by a CoA ligase from Penicillium chrysogenum. The enzyme accepted medium-chain length fatty acids as the best substrates, but the p...

journal_title：FEBS letters

authors： Koetsier MJ,Jekel PA,Wijma HJ,Bovenberg RA,Janssen DB

更新日期：2011-03-23 00:00:00

abstract：:Sarcoplasmic reticulum vesicles were solubilized stepwise by the nonionic detergent octaethyleneglycol monododecyl ether; 31P NMR enabled the extent of phospholipid solubilization to be monitored by following the conversion of the broad resonance peak characterizing the phospholipids inserted in the bilayer to the nar...

journal_title：FEBS letters

authors： Roux M,Champeil P

更新日期：1984-06-11 00:00:00

abstract：:Many genes are regulated by the intracellular calcium, protein kinase C (PKC) and protein kinase A (PKA) pathways and it has been shown that these pathways synergize in some cell types, whereas they antagonize in others. Here we show that the calcium and PKC pathways suppress the effects mediated by the PKA pathway in...

journal_title：FEBS letters

authors： Döbbeling U,Berchtold MW

更新日期：1996-08-05 00:00:00

abstract：:Carboxypeptidase H (CPH) is one of several processing enzymes required for the conversion of peptide hormone precursors into their smaller active forms. In this study, high levels of CPH activity was found in a liver metastasis of a human ileal carcinoid which expresses beta-preprotachykinin mRNA and the tachykinin ne...

journal_title：FEBS letters

authors： Hook VY,Affolter HU

更新日期：1988-10-10 00:00:00

abstract：:Bovine insulin can be split into several fractions by gel filtration on Sephadex G50. These are, in order of decreasing molecular weight, a new unidentified substance, proinsulin, and a large fraction containing several substances. Ultracentrifugal examination of the proinsulin in acetic acid solution (pH 2.3) gave a ...

journal_title：FEBS letters

authors： Zühlke H,Behlke J

更新日期：1968-12-01 00:00:00

abstract：:In Graves' disease (GD) the presence of antibodies to the thyroid stimulating hormone (TSH) receptor leads to stimulation of the thyroid gland. The thyroid stimulating activity of Graves' IgG is normally ascertained by bioassays measuring cAMP production. We have investigated the effect of Graves' IgG on the quantitat...

journal_title：FEBS letters

authors： Kung AW,Collison K,Banga JP,McGregor AM

更新日期：1988-05-09 00:00:00

abstract：:During lactation glucose metabolism in paraovarian adipocytes is characterized by a 40 and 80% decrease of glucose incorporation into CO2 and fatty acids in the presence of insulin. In contrast with the stimulation by insulin of glucose incorporation into lactate, glycerol remains unchanged. As a result, insulin sensi...

journal_title：FEBS letters

authors： Burnol AF,Guerre-Millo M,Lavau M,Girard J

更新日期：1986-01-06 00:00:00

abstract：:The single cysteinyl residue 26 of spinach calmodulin was labelled with the thiol-specific bimane fluorescence probe. Following application of stoichiometric quantities of Ca2+ or aluminum ions to the protein, temperature-dependent fluorescence changes (anisotropy, lifetime) could be monitored via the label. From thes...

journal_title：FEBS letters

authors： Yuan SX,Haug A

更新日期：1988-07-04 00:00:00

abstract：:The metabolite-modulated association of a fraction of hexokinase to mitochondria in brain is well documented, however, the involvement of other non-mitochondrial components in the binding of the hexokinase is controversial. Now we present evidence that the hexokinase binds both tubulin and microtubules in brain in vit...

journal_title：FEBS letters

authors： Wágner G,Kovács J,Löw P,Orosz F,Ovádi J

更新日期：2001-11-30 00:00:00

abstract：:To test if mitochondrial uncoupling in white adipocytes is responsible for obesity resistance of the aP2-Ucp transgenic mice expressing ectopic uncoupling protein 1 (UCPI) in white fat, mitochondrial membrane potential (delta psi(m)) was estimated by flow cytometry in adipocytes isolated from gonadal fat. Ectopic UCP1...

journal_title：FEBS letters

authors： Baumruk F,Flachs P,Horáková M,Floryk D,Kopecký J

更新日期：1999-02-12 00:00:00

abstract：:Here, we report the volume and enthalpy changes accompanying CO photodissociation from the mixed valence form of cytochrome bo3 oxidase from Escherichia coli. The results of photoacoustic calorimetry indicate two kinetic phases with distinct volume and enthalpy changes accompanying CO photodissociation from heme o3 an...

journal_title：FEBS letters

authors： Miksovská J,Gennis RB,Larsen RW

更新日期：2005-06-06 00:00:00

abstract：:Beta-thymosins sequester G-actin and preserve a pool of monomers of actin which constitute an important prerequisite for cellular function of the microfilament system. To study the influence of paraquat binding to G-actin on the interaction of G-actin with thymosin beta4 we determined the apparent dissociation constan...

journal_title：FEBS letters

authors： Huff T,Cappelletti G,Hannappel E

更新日期：1998-04-03 00:00:00

abstract：:Spectral variants of the green fluorescent protein (GFP) have been extensively used as reporters to image molecular interactions in living cells by fluorescence resonance energy transfer (FRET). However, those GFP variants which are the most efficient donor acceptor pairs for FRET measurements show a high degree of sp...

journal_title：FEBS letters

authors： Zimmermann T,Rietdorf J,Girod A,Georget V,Pepperkok R

更新日期：2002-11-06 00:00:00

abstract：:VacA is a major virulence factor of the widespread stomach-dwelling bacterium Helicobacter pylori. It causes cell vacuolation and tissue damage by forming anion-selective, urea-permeable channels in plasma and endosomal membranes. We report that several flavone derivatives and other polyphenols present in vegetables a...

journal_title：FEBS letters

authors： Tombola F,Campello S,De Luca L,Ruggiero P,Del Giudice G,Papini E,Zoratti M

更新日期：2003-05-22 00:00:00

abstract：:Early in the course of pregnancy, at the preimplantation stage, the pig embryo is likely to exert a paracrine effect on the tissue intended to receive it, via the secretion of interferons. Our observations show that trophoblastic interferons induce an increase of some mRNAs in the epithelial cells of the gilt endometr...

journal_title：FEBS letters

authors： Chwetzoff S,d'Andrea S

更新日期：1997-03-24 00:00:00

abstract：:Streptococcus pneumoniae (pneumococcus) has evolved sophisticated strategies to survive in several niches within the human body either as a harmless commensal or as a serious pathogen causing a variety of diseases. The dynamic interaction between pneumococci and resident host cells during colonization of the upper res...

journal_title：FEBS letters

authors： Kohler S,Voß F,Gómez Mejia A,Brown JS,Hammerschmidt S

更新日期：2016-11-01 00:00:00

abstract：:Treatment of H. chlorum membrane preparations with diethyl ether of high degrees of water saturation raised the bacteriochlorophyll (BChl) g' mole fraction, as determined by HPLC analysis of their acetone extracts, toward a level of 40% of total BChl g or higher. Starting from pure BChl g, the BChl g' mole fraction sh...

journal_title：FEBS letters

authors： Kobayashi M,Watanabe T,Ikegami I,van de Meent EJ,Amesz J

更新日期：1991-06-17 00:00:00

abstract：:Melittin, deuteromethylated on each of the four amino groups (Gly-1 N alpha and Lys-7, 21, and 23 N epsilon), was prepared by reductive methylation using deuteroformaldehyde and NaBD3CN. Deuterium NMR spectra were obtained for the modified peptide (D-melittin) bound to phospholipid bilayers and erythrocyte ghosts. D-M...

journal_title：FEBS letters

authors： Dempsey CE,Cryer GD,Watts A

更新日期：1987-06-22 00:00:00

abstract：:Previously, we found that intracellular reactive oxygen species (ROS) affect photomorphogenesis in Neurospora crassa. In this study, we investigated the physiological roles of ROS in the response to light and found that the exposure of mycelia to air was important for the light-induced carotenogenesis. Mycelia treated...

journal_title：FEBS letters

authors： Iigusa H,Yoshida Y,Hasunuma K

更新日期：2005-07-18 00:00:00

abstract：:Vacuolar H(+)-PPase, a membrane bound proton-translocating pyrophosphatase found in various species including plants, some protozoan and prokaryotes, has been demonstrated to be localized to the vacuolar membrane in plants. Using a GUS reporter system and a green fluorescent protein (GFP) fusion protein, we investigat...

journal_title：FEBS letters

authors： Mitsuda N,Enami K,Nakata M,Takeyasu K,Sato MH

更新日期：2001-01-12 00:00:00

abstract：:We previously showed that heat stress stimulates reactive oxygen species (ROS) production in skeletal muscle mitochondria of birds, probably via an elevation in mitochondrial membrane potential (DeltaPsi). To clarify the mechanism underlying the elevation of DeltaPsi, modular kinetic analysis was applied to oxidative ...

journal_title：FEBS letters

authors： Kikusato M,Ramsey JJ,Amo T,Toyomizu M

更新日期：2010-07-16 00:00:00

abstract：:The molecular basis and functional properties of a variant antithrombin (AT) protein. AT Budapest 3, were studied. A single base substitution was identified in codon 99, CTC----TTC, altering the normal leucine to phenylalanine. The proband presented with a history of venous thrombotic disease and was found to be homoz...

journal_title：FEBS letters

authors： Olds RJ,Lane DA,Boisclair M,Sas G,Bock SC,Thein SL

更新日期：1992-04-06 00:00:00

abstract：:Cell migration over or through the extracellular matrix (ECM) is an integral feature of both physiological and pathological processes. Regulation of the changing cell-ECM interactions involved can be effected by proteolysis and requires strict spatial and temporal targeting of proteinase activity. The versatile use of...

journal_title：FEBS letters

authors： Ellis V,Murphy G

更新日期：2001-09-28 00:00:00

abstract：:Retroviruses have been etiologically implicated with leukemia in humans and animals. Understanding the virus life cycle, the proteins and enzymes involved in its replication, is essential for developing potent anti-viral drugs. Phosphorylation of retroviral proteins may alter their shape in such a way as to increase o...

journal_title：FEBS letters

authors： Seri I,Priel E

更新日期：1996-09-02 00:00:00

abstract：:A metallothionein cDNA was generated from pea (Pisum sativum L.) roots, amplified by PCR and inserted into a plasmid for expression in E. coli. Purification of the resultant product generated 3 pools of cadmium-containing material after DEAE-cellulose chromatography. The amino acid composition of each was in excellent...

journal_title：FEBS letters

authors： Kille P,Winge DR,Harwood JL,Kay J

更新日期：1991-12-16 00:00:00

abstract：:A recombinant antibody light chain (L chain) maintained under non-denaturing conditions displayed preferential cleavage of synthetic peptides conjugated to methylcoumarinamide (MCA) on the C-terminal side of Arg and Lys residues. The same L chain renatured from a denaturing solvent (guanidine hydrochloride) acquired t...

journal_title：FEBS letters

authors： Sun M,Paul S

更新日期：1997-05-05 00:00:00

abstract：:Extensive data on the glycosaminoglycan (GAG) composition and the collagen fibril diameter distribution have been collected for a diverse range of connective tissues. It is shown that tissues with the smallest diameter collagen fibrils (mass-average diameter less than 60 nm) have high concentrations of hyaluronic acid...

journal_title：FEBS letters

authors： Parry DA,Flint MH,Gillard GC,Craig AS

更新日期：1982-11-22 00:00:00

abstract：:The effects of progesterone, dehydroepiandrosterone sulfate and 17beta-estradiol on the synthesis and degradation of hyaluronate were investigated using human uterine cervix fibroblasts. The cells were incubated with [3H]glucosamine in the presence of the hormones and then [3H]hyaluronate was isolated from the medium....

journal_title：FEBS letters

authors： Tanaka K,Nakamura T,Takagaki K,Funahashi M,Saito Y,Endo M

更新日期：1997-02-03 00:00:00

abstract：:It is 40 years since cancer growth was correlated with neovascularisation. Anti-angiogenic drugs remain at the forefront of cancer investigations but progress has been disappointing and unexpected toxicities are emerging. Gap junction channels are implicated in lesion spread following injury, with channel blockers sho...

journal_title：FEBS letters

authors： Zhang J,O'Carroll SJ,Henare K,Ching LM,Ormonde S,Nicholson LF,Danesh-Meyer HV,Green CR

更新日期：2014-04-17 00:00:00