Abstract:
:Here, we report the volume and enthalpy changes accompanying CO photodissociation from the mixed valence form of cytochrome bo3 oxidase from Escherichia coli. The results of photoacoustic calorimetry indicate two kinetic phases with distinct volume and enthalpy changes accompanying CO photodissociation from heme o3 and its transfer to CuB. The first phase occurring on a timescale of <50 ns is characterized by a volume decrease of -1.3+/-0.3 mL mol-1 and enthalpy change of 32+/-1.6 kcal mol-1. Subsequently, a volume increase of 2.9 mL mol-1 with an enthalpy change of -5.3+/-2.5 kcal mol-1 is observed with the lifetime of approximately 250 ns (this phase has not been detected in previous optical studies). These volume and enthalpy changes differ from the volume and enthalpy changes observed for CO dissociation from fully reduced cytochrome bo3 oxidase indicating that the heme o3/CuB active site dynamics are affected by the redox state of heme b.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Miksovská J,Gennis RB,Larsen RWdoi
10.1016/j.febslet.2005.04.055keywords:
subject
Has Abstractpub_date
2005-06-06 00:00:00pages
3014-8issue
14eissn
0014-5793issn
1873-3468pii
S0014-5793(05)00531-4journal_volume
579pub_type
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