Conserved core tryptophans of FnII domains are crucial for the membranolytic and chaperone-like activities of bovine seminal plasma protein PDC-109.

abstract：:Bovine globin has been incubated with mice peritoneal macrophages in order to study its hydrolysis by lysosomal enzymes, among which chiefly cathepsin D. Analysis of resulting peptides, by reversed-phase high-performance liquid chromatography (RP-HPLC), showed the release of a bioactive peptide, VV-hemorphin-7. When a...

journal_title：FEBS letters

authors： Dagouassat N,Garreau I,Sannier F,Zhao Q,Piot JM

更新日期：1996-03-11 00:00:00

abstract：:The mutation of Asp198 to Asn in the receptor for glucagon-like peptide-1(7-36)amide (GLP-1) had no effect upon GLP-1 affinity whereas substitution with Ala greatly reduced affinity, demonstrating the importance of polarity rather than negative charge at Asp198. However, the Asp198-Ala mutation had less effect upon th...

journal_title：FEBS letters

authors： López de Maturana R,Donnelly D

更新日期：2002-10-23 00:00:00

abstract：:Gemin5 acts as a U1 small nuclear RNA (snRNA)-binding protein in U1 small nuclear ribonucleic protein (snRNP) biogenesis. Here, we report a role for Gemin5 in unassembled U1 snRNP disposal under survival of motor neuron (SMN) protein-deficient conditions. We demonstrate that non-Sm protein-associated U1 snRNA and U1A ...

journal_title：FEBS letters

authors： Jiang D,Zou X,Zhang C,Chen J,Li Z,Wang Y,Deng Z,Wang L,Chen S

更新日期：2018-04-01 00:00:00

abstract：:Accumulation of unfolded proteins in the endoplasmic reticulum (ER) causes ER stress. The ER stress sensor inositol requiring enzyme-1beta (IRE1β), which is specifically expressed in intestinal epithelial cells, is thought to be involved in translational repression. However, its mechanism of action is not fully unders...

journal_title：FEBS letters

authors： Nakamura D,Tsuru A,Ikegami K,Imagawa Y,Fujimoto N,Kohno K

更新日期：2011-01-03 00:00:00

abstract：:The protein kinase C activator, phorbol-12-myristate-13-acetate (PMA), augments the cyclic AMP accumulation induced by forskolin in pheochromocytoma (PC 12) cells with an EC50 value of 14 nM, while having no effect on basal values. At a concentration of 100 nM PMA markedly augmented the magnitude of the forskolin resp...

journal_title：FEBS letters

authors： Hollingsworth EB,Ukena D,Daly JW

更新日期：1986-02-03 00:00:00

abstract：:Vascular smooth muscle cell (VSMC) proliferation plays a key role in neointimal hyperplasia and restenosis. Here we report the role of the microRNA miR-142-5p and its downstream target genes on the proliferation of cultured VSMCs. miR-142-5p promoted VSMC proliferation by down-regulating B cell translocation gene 3 (B...

journal_title：FEBS letters

authors： Kee HJ,Park S,Kwon JS,Choe N,Ahn Y,Kook H,Jeong MH

更新日期：2013-08-02 00:00:00

abstract：:Phlorizin, a potent inhibitor of the Na+/D-glucose cotransporter, was derivatised to 3-aminophlorizin and subsequently coupled to Affi-Gel 15. Affinity chromatography of pig kidney brush border membranes solubilised in Triton X-100 allowed the purification of a 60 kDa protein on this resin. We consider this protein to...

journal_title：FEBS letters

authors： Kitlar T,Morrison AI,Kinne R,Deutscher J

更新日期：1988-07-04 00:00:00

abstract：:We previously isolated a Saccharomyces cerevisiae mutant (HsTnII), which displays 40% reduced chronological lifespan as compared to the wild type (WT). In this study, we found HsTnII cultures to be characterized by fragmented and dysfunctional mitochondria, and by increased initiation of apoptosis during chronological...

journal_title：FEBS letters

authors： Aerts AM,Zabrocki P,Govaert G,Mathys J,Carmona-Gutierrez D,Madeo F,Winderickx J,Cammue BP,Thevissen K

更新日期：2009-01-05 00:00:00

abstract：:To date the only tRNAs containing nucleosides modified with a selenium (5-carboxymethylaminomethyl-2-selenouridine and 5-methylaminomethyl-2-selenouridine) have been found in bacteria. By using tRNA anticodon-stem-loop fragments containing S2U, Se2U, or geS2U, we found that in vitro tRNA 2-selenouridine synthase (SelU...

journal_title：FEBS letters

authors： Sierant M,Leszczynska G,Sadowska K,Komar P,Radzikowska-Cieciura E,Sochacka E,Nawrot B

更新日期：2018-07-01 00:00:00

abstract：:Pin2/TRF1 was independently identified as a telomeric DNA-binding protein (TRF1) that regulates telomere length, and as a protein (Pin2) that can bind the mitotic kinase NIMA and suppress its lethal phenotype. We have previously demonstrated that Pin2/TRF1 levels are cell cycle-regulated and its overexpression induces...

journal_title：FEBS letters

authors： Nakamura M,Zhou XZ,Kishi S,Lu KP

更新日期：2002-03-13 00:00:00

abstract：:The promoter of the cholecystokinin (CCK) gene possesses evolutionary conserved juxtaposed E-box and cAMP/TPA responsive elements (CRE/TRE). We have examined the functional interaction of these two sites. As previously noted, c-Jun/c-Fos heterodimers greatly increase promoter activity through association with the CRE/...

journal_title：FEBS letters

authors： Rourke IJ,Hansen TV,Nerlov C,Rehfeld JF,Nielsen FC

更新日期：1999-04-01 00:00:00

abstract：:Type II collagen is the major protein of cartilage and is synthesized as a procollagen in two forms (IIA and IIB), generated by differential splicing of the gene primary transcript. Previous studies have indicated that only type IIB is expressed in differentiated chondrocytes. Here, we examined the effects of bone mor...

journal_title：FEBS letters

authors： Valcourt U,Gouttenoire J,Aubert-Foucher E,Herbage D,Mallein-Gerin F

更新日期：2003-06-19 00:00:00

abstract：:Biological activities of five synthetic lipid A analogues (D-glucosamine derivatives) were examined to elucidate the structure required for expression of the biological activities of endotoxin. Proclotting enzyme of horseshoe crab activation, interferon-inducing and tumor necrosis factor-inducing activities were signi...

journal_title：FEBS letters

authors： Matsuura M,Kojima Y,Homma JY,Kubota Y,Yamamoto A,Kiso M,Hasegawa A

更新日期：1984-02-27 00:00:00

abstract：:The sequence of seminalplasmin, a basic antimicrobial and transcription-inhibitory protein from bovine seminal plasma, has been determined using an automated sequenator. This sequence is slightly different from that reported earlier by Theil and Scheit [(1983) EMBO J. 2, 1159-1163] and identical with that of caltrin, ...

journal_title：FEBS letters

authors： Sitaram N,Kumari VK,Bhargava PM

更新日期：1986-06-09 00:00:00

abstract：:Hydrogenase gene from Clostridium butyricum was cloned in Escherichia coli HK16 (Hyd-) using pBR322 and PstI. The plasmid, pCBH1, containing hydrogenase gene was 7.3 MDa and pCBH1 had 5 PstI-DNA fragments (3.9, 2.6, 0.7, 0.03-0.04, less than 0.02 MDa, respectively). The hydrogenase activity of HK16 (pCBH1) was about 3...

journal_title：FEBS letters

authors： Karube I,Urano N,Yamada T,Hirochika H,Sakaguchi K

更新日期：1983-07-11 00:00:00

abstract：:Intravital multiphoton imaging allows visualization of infections and pathogenic mechanisms within intact organs in their physiological context. Today, most organs of mice and rats are applicable to in vivo or ex vivo imaging, opening completely new avenues for many researchers. Advances in fluorescent labeling of pat...

journal_title：FEBS letters

authors： Stolp B,Melican K

更新日期：2016-07-01 00:00:00

abstract：:The RhoGTPase Rac1 is activated in a polarised fashion and controls cell motility. We previously showed that Rac1 binds the PP2A inhibitor SET and recruits nuclear SET to the cytosol. We show that a SET mutant, lacking a nuclear localization signal, SET(ΔNLS), promotes cell spreading and motility. This was accompanied...

journal_title：FEBS letters

authors： Lam BD,Anthony EC,Hordijk PL

更新日期：2013-01-16 00:00:00

abstract：:Constitutively secreted proteins have traditionally been believed to be excluded from the regulated secretory pathway. In this work we show that kappa light chain and Fc fragment, two markers of the constitutive pathway, are present in the regulated pathway in AtT-20 cells. They colocalize with the endogenous hormone ...

journal_title：FEBS letters

authors： Castle AM,Huang AY,Castle JD

更新日期：1998-11-20 00:00:00

abstract：:In PC12 cells, cAMP stimulates the MAP kinase pathway by an unknown mechanism. Firstly, we examined the role of calcium ion mobilization and of protein kinase C in cAMP-stimulated MAP kinase activation. We show that cAMP stimulates p44mapk independently of these events. Secondly, we studied the role of B-Raf in this p...

journal_title：FEBS letters

authors： Peraldi P,Frödin M,Barnier JV,Calleja V,Scimeca JC,Filloux C,Calothy G,Van Obberghen E

更新日期：1995-01-09 00:00:00

abstract：:Haloalkane dehalogenase hydrolyses various 1-halo-n-alkanes to the corresponding alcohols by covalent catalysis with formation of an alkyl-enzyme intermediate. The carboxylate function of the nucleophilic aspartate (Asp-124) that displaces the halogen during formation of the intermediate was changed to an amide by sit...

journal_title：FEBS letters

authors： Pries F,Kingma J,Janssen DB

更新日期：1995-01-23 00:00:00

abstract：:Rat calvaria cells grown in culture for one week had properties of osteoblasts: a high content in alkaline phosphatase and a marked cyclic AMP response to parathyroid hormone (PTH). In short-term experiments, insulin-like growth factor I (IGF I) stimulated the incorporation of [14C] glucose into glycogen. When IGF I w...

journal_title：FEBS letters

authors： Schmid C,Steiner T,Froesch ER

更新日期：1984-07-23 00:00:00

abstract：:Accurate cDNA data is useful to validate gene structures in a genome. We sequenced 35189 expressed sequence tags (ESTs) obtained from the highly destructive rice blast fungus, Magnaporthe grisea. Our custom-made computational programs mapped these ESTs on the M. grisea genome sequence, and reconstructed gene structure...

journal_title：FEBS letters

authors： Numa H,Nishimura M,Tanaka T,Kanamori H,Yang CC,Matsumoto T,Nagamura Y,Itoh T

更新日期：2009-02-18 00:00:00

abstract：:Immature Drosophila rhodopsin is N-glycosylated, but undergoes complete deglycosylation during the process of protein maturation. In order to elucidate the site of glycosylation and its role in rhodopsin synthesis, we investigated the in vitro and in vivo synthesis of rhodopsin whose putative N-glycosylation sites (As...

journal_title：FEBS letters

authors： Katanosaka K,Tokunaga F,Kawamura S,Ozaki K

更新日期：1998-03-13 00:00:00

abstract：:Several deletion mutants of Raf-1 were expressed with v-ras p21 or 14-3-3 protein in COS-7 cells and Sf9 cells and the interaction of Raf-1 with ras p21 or with 14-3-3 protein in intact cells was examined. Raf(1-135) (residues 1-135) and Raf(1-322) interacted with v-ras p21, but other deletion mutants such as Raf(136-...

journal_title：FEBS letters

authors： Koyama S,Williams LT,Kikuchi A

更新日期：1995-07-17 00:00:00

abstract：:The fluorescence dye 1-anilinonaphthalene-8-sulfonate (ANS) was used as a probe of non-polar binding sites in 4-aminobutyrate aminotransferase. ANS binds to a single binding site of the dimeric protein with a Kd of 6 microM. Nanosecond emission anisotropy measurements were performed on the ANS-enzyme in an effort to d...

journal_title：FEBS letters

authors： Churchich JE,Kim DS,Schnackerz KD

更新日期：1983-08-22 00:00:00

abstract：:We have studied whether the cooperative insecticidal effect of certain scorpion toxin pairs, namely either a combination of excitatory and depressant, or alpha and depressant scorpion toxins, would improve the efficacy of Autographa californica nucleopolyhedrovirus (AcMNPV) over a virus expressing only a single toxin,...

journal_title：FEBS letters

authors： Regev A,Rivkin H,Inceoglu B,Gershburg E,Hammock BD,Gurevitz M,Chejanovsky N

更新日期：2003-02-27 00:00:00

abstract：:The fluorescent calcium indicator 'quin2' was used to demonstrate changes in cytoplasmic calcium concentrations in bovine anterior pituitary cells. The basal calcium concentration was 0.21 +/- 0.02 microM (mean of 4 cell preparations). Thyroliberin (TRH) (10(-10) - 10(-6) M) rapidly and at the higher concentrations tr...

journal_title：FEBS letters

authors： Schofield JG

更新日期：1983-08-08 00:00:00

abstract：:Cellular retinoic acid binding proteins (CRABPs) bind all-trans-retinoic acid (atRA) tightly. This study aimed to determine whether atRA is channeled directly to cytochrome P450 (CYP) CYP26B1 by CRABPs, and whether CRABPs interact directly with CYP26B1. atRA bound to CRABPs (holo-CRABP) was efficiently metabolized by ...

journal_title：FEBS letters

authors： Nelson CH,Peng CC,Lutz JD,Yeung CK,Zelter A,Isoherranen N

更新日期：2016-08-01 00:00:00

abstract：:Apoptosis is a physiological process for active cell removal. One of its hallmarks is an increased cytosolic Ca2+ content. Several genes involved in apoptosis control have been identified, but their mode of action is not understood in detail. Apoptosis may relate to oncogenesis, in that some malignant tumors may grow ...

journal_title：FEBS letters

authors： Richter C

更新日期：1993-06-28 00:00:00

abstract：:The polytopic transmembrane protein, Niemann-Pick C1-Like 1 (NPC1L1), is enriched in the apical membrane of small intestine absorptive enterocytes where it mediates extracellular sterol transport across the brush border membrane. It is essential for intestinal sterol absorption and is the molecular target of ezetimibe...

journal_title：FEBS letters

authors： Betters JL,Yu L

更新日期：2010-07-02 00:00:00