Abstract:
:The fluorescent calcium indicator 'quin2' was used to demonstrate changes in cytoplasmic calcium concentrations in bovine anterior pituitary cells. The basal calcium concentration was 0.21 +/- 0.02 microM (mean of 4 cell preparations). Thyroliberin (TRH) (10(-10) - 10(-6) M) rapidly and at the higher concentrations transiently increased the concentration. Dopamine (10(-10) - 10(-7) M) decreased the concentration transiently and more slowly. At 10(-5) M, dopamine prevented the increase in calcium concentration caused by 10(-9) M TRH, and partially inhibited the increase caused by higher concentrations of the peptide. The data support the hypothesis that calcium is the second messenger for TRH, and suggest that dopamine inhibits TRH-induced prolactin secretion by preventing the calcium concentration from exceeding the level necessary to increase secretion.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Schofield JGdoi
10.1016/0014-5793(83)80420-7subject
Has Abstractpub_date
1983-08-08 00:00:00pages
79-82issue
1-2eissn
0014-5793issn
1873-3468pii
0014-5793(83)80420-7journal_volume
159pub_type
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