Low molecular mass GTP-binding proteins of adrenal chromaffin cells are present on the secretory granule.

Abstract:

:Adrenal medullary homogenates and chromaffin granule membranes were separated by SDS-polyacrylamide gel electrophoresis and GTP-binding proteins detected using [alpha-32P]GTP binding to nitrocellulose blots. Four GTP-binding polypeptides of 24, 22, 20 and 18 kDa were routinely found in medullary homogenates and all were also found in isolated chromaffin granule membranes. The GTP-binding polypeptides co-sedimented with granule membrane markers following separation on sucrose gradients. On the basis of trypsin sensitivity and resistance to extraction, the GTP-binding proteins appeared to be tightly bound to the cytoplasmic surface of the granules. One or more of the secretory granule GTP-binding proteins could be involved in exocytosis in adrenal chromaffin cells.

journal_name

FEBS Lett

journal_title

FEBS letters

authors

Burgoyne RD,Morgan A

doi

10.1016/0014-5793(89)80204-2

subject

Has Abstract

pub_date

1989-03-13 00:00:00

pages

122-6

issue

1-2

eissn

0014-5793

issn

1873-3468

pii

0014-5793(89)80204-2

journal_volume

245

pub_type

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