Abstract:
:We present here a new set of aminoglycoside-arginine conjugates (AACs) that are either site-specific or per-arginine conjugates of paromomycin, neamine, and neomycin B as well as their structure-activity relationships. Their binding constants (KD) for TAR and RRE RNAs, measured by fluorescence anisotropy, revealed dependence on the number and location of arginines in the different aminoglycoside conjugates. The binding affinity of the per-arginine aminoglycosides to TAR is higher than to RRE, and hexa-arginine neomycin B is the most potent binder (KD=5 and 23 nM, respectively). The 2D TOCSY NMR spectrum of the TAR monoarginine-neomycin complex reveals binding at the bulge region of TAR.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Lapidot A,Vijayabaskar V,Litovchick A,Yu J,James TLdoi
10.1016/j.febslet.2004.10.038keywords:
subject
Has Abstractpub_date
2004-11-19 00:00:00pages
415-21issue
3eissn
0014-5793issn
1873-3468pii
S0014579304012761journal_volume
577pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::The determination of protein structure by NMR is restricted at molecular masses above 10 kDa by overlapping resonances. One way of overcoming this problem is to label the protein with 15N. The conventional way to record 15N spectra is to use heteronuclear multiple-quantum coherence. We present here an alternative appr...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)81124-x
更新日期:1989-09-25 00:00:00
abstract::HslVU is an ATP-dependent protease consisting of two multimeric components, the HslU ATPase and the HslV peptidase. To gain an insight into the role of HslVU in regulation of cell division, the reconstituted enzyme was incubated with SulA, an inhibitor of cell division in Escherichia coli, or its fusion protein with m...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00935-7
更新日期:1999-07-30 00:00:00
abstract::Human nuclear cyclophilin 33 (hCyP33) was the first protein which was found to contain an RNA-binding motif and a PPIase domain. It was not known what cellular and physiological roles are played by the RNA-binding activity as well as the PPIase activity of hCyP33. In this paper, we investigated the binding specificity...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2008.01.055
更新日期:2008-03-05 00:00:00
abstract::The NFkappaB family of transcription factors is regulated by inhibitory IkappaB proteins. A diversity of stimuli leads to the phosphorylation and subsequent degradation of IkappaB, releasing NFkappaB to act on its target genes. Calmodulin (CaM) is a key regulator of numerous cellular processes and is the predominant i...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)01537-3
更新日期:1998-12-11 00:00:00
abstract::The role of adenosine 5'-triphosphate (ATP) and P2Y(1) nucleotide receptor in potentiating agrin-induced acetylcholine receptor (AChR) aggregation is being demonstrated in a co-culture system of NG108-15 cell, a mouse neuroblastoma X rat glioma hybrid cell line that resembles spinal motor neuron, with C2C12 myotube. I...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.03.054
更新日期:2005-04-25 00:00:00
abstract::The proteolysis of beta-amyloid (Abeta) requires neprylisin, an enzyme that has been shown as reduced in Alzheimer's disease (AD). We investigated whether a decrease in neprilysin levels contributes to the accumulation of amyloid deposits not only in AD but also in the normal aging. We analyzed neprilysin mRNA and pro...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.09.054
更新日期:2005-11-07 00:00:00
abstract::Methyl-beta-cyclodextrin (MbetaCD) was used to explore a role for cholesterol-enriched plasma membrane microdomains in coupling lysophosphatidic acid (LPA) stimulation to phosphoinositide 3-kinase (PI3K) activation. Cholesterol depletion strongly inhibited the production of phosphatidylinositol 3,4-bisphosphate and ph...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)03832-2
更新日期:2003-01-16 00:00:00
abstract::Hepatitis B virus X protein is a major factor in the HBV-induced disease developments. Stromal cell-derived factor-1 is a small cytokine that is strongly chemotactic for lymphocytes. We explored the role of HBx on recruitment of HBV-induced virus-nonspecific immune cells into liver. Immune cell recruitment and SDF-1 e...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2014.01.017
更新日期:2014-03-03 00:00:00
abstract::The possible role of sphingomyelin cycle for the regulation of cell proliferation was investigated in human keratinocytes. The time-dependent breakdown of sphingomyelin was observed in the immortalized human keratinocyte cell line HaCaT as well as in primary human keratinocytes thereby providing evidence that the sphi...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)01421-7
更新日期:1996-01-02 00:00:00
abstract::We have studied the intracellular distribution in vivo of glucokinase (GK) and glucokinase regulatory protein (GKRP) in livers of fasted and refed rats, using specific antibodies against both proteins and laser confocal fluorescence microscopy. GK was found predominantly in the nucleus of hepatocytes from starved rats...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)01249-1
更新日期:1999-10-08 00:00:00
abstract::The structures of the M110 and M21 regulatory subunits of protein phosphatase-1M, the major enzyme which dephosphorylates myosin in smooth muscle, have been deduced from cloned cDNAs. The N-terminus of the M110 subunit from rat aorta contains seven ankyrin repeats, while the C-terminus of the M21 subunit from chicken ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)01231-8
更新日期:1994-12-12 00:00:00
abstract::A simple method was developed for the isolation of primase-free DNA polymerase-alpha from the DNA polymerase-alpha-primase complex of mouse FM3A cells. The polymerase was separated from primase subunits by chromatography on a single-stranded DNA-cellulose column in the presence of 50% etylene glycol. The primase-free ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)81043-n
更新日期:1990-10-29 00:00:00
abstract::Hydrolysis of GTP, bound to members of the G-protein superfamily, terminates their downstream signaling activity. A conserved glutamine serves a critical role in this pivotal guanosine triphosphatase (GTPase) reaction. However, the role of the catalytic glutamine in GTP hydrolysis is still not well understood. We have...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)00930-2
更新日期:1998-08-21 00:00:00
abstract::A conformational transition between E2 and E1 forms of Na, K-ATPase induced by different nucleotides has been studied under steady state conditions using the enzyme labelled with 5-iodoacetamidofluorescein. In the presence of K+ the plot of fluorescence as a function of [ATP], [ADP] or [CTP] (in a range of 5 microM-12...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)81510-u
更新日期:1990-06-18 00:00:00
abstract::The RhoGTPase Rac1 is activated in a polarised fashion and controls cell motility. We previously showed that Rac1 binds the PP2A inhibitor SET and recruits nuclear SET to the cytosol. We show that a SET mutant, lacking a nuclear localization signal, SET(ΔNLS), promotes cell spreading and motility. This was accompanied...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2012.11.013
更新日期:2013-01-16 00:00:00
abstract::The 500 MHz 1H NMR spectrum of a 68-residue peptide, encompassing the rat thyroid transcription factor 1 homeodomain (TTF-1 HD), was fully assigned using standard 2D NMR methodology. The secondary structure elements and their spatial organization were determined and led to a structure very similar to that previously d...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)80845-l
更新日期:1993-12-28 00:00:00
abstract::Preillumination of intact cells of the eukaryotic, halotolerant, cell-wall-less green alga Dunaliella salina induces a dark ATPase activity the magnitude of which is about 3-5-fold higher than the ATPase activity observed in dark-adapted cells. The light-induced activity arises from the activation and stabilization in...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)80632-x
更新日期:1988-03-28 00:00:00
abstract::Initiation of translation in hepatitis C virus (HCV) is dependent on the presence of an internal ribosome entry site (IRES) contained in its 341-nt-long 5'-untranslated region (UTR). This region is very conserved among different isolates and has been used to classify HCV isolates in six different genotypes. These geno...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)00715-1
更新日期:1997-07-14 00:00:00
abstract::Three mutant polypeptides of the type I 37-residue winter flounder 'antifreeze' protein have been synthesized. All four threonine residues in the native peptide were been mutated to serine, valine and glycine respectively and two additional salt bridges were incorporated into the sequences in order to improve aqueous ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)00652-8
更新日期:1998-07-03 00:00:00
abstract::Here we report the first crystal structure of a protein, AmyA, a secretory alpha-amylase isolated from Halothermothrix orenii, which is both halophilic and thermophilic. The crystal structure was determined at 1.6 A resolution. AmyA lacks the conserved acidic surface, which is considered essential for protein stabilit...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.04.017
更新日期:2006-05-15 00:00:00
abstract::Cytochrome oxidase o has been isolated from the obligately aerobic, methylotrophic bacterium Methylophilus methylotrophus in the form of a cytochrome cL-o complex. The latter is comprised of cytochrome cL (Mr 21 000) and cytochrome o (Mr 29 000) in a 1-2:1 ratio, possibly in association with one or more minor polypept...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(82)80599-1
更新日期:1983-05-08 00:00:00
abstract::We present an open-access web platform isomiRex, to identify isomiRs and on the fly graphical visualization of the differentially expressed miRNAs in control as well as treated library. The open-access web-platform is not restricted only to NGS sequence dataset from animals and potentially analyzes a wider dataset for...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2013.06.047
更新日期:2013-08-19 00:00:00
abstract::TP53 belongs to a small gene family that includes, in mammals, two additional paralogs, TP63 and TP73. The p63 and p73 proteins are structurally and functionally similar to p53 and their activity as transcription factors is regulated by a wide repertoire of shared and unique post-translational modifications and intera...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2014.06.047
更新日期:2014-08-19 00:00:00
abstract::The nef gene of human and simian immunodeficiency virus is a key factor in acquired immunodeficiency syndrome pathogenesis and virus replication. Several Nef-induced phenomena, including the down-regulation of CD4 molecule, have been previously reported. In this study, we have identified and cloned a novel cellular pr...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)01631-7
更新日期:1999-01-08 00:00:00
abstract::A novel type IV collagen-degrading metalloproteinase was purified from the conditioned media of a murine metastatic sarcoma cell line. The molecular weight of the purified enzyme was determined to be 100 kDa by SDS-PAGE, while 700 kDa by gel filtration suggesting that the enzyme has a multimer structure. This enzyme d...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)80967-n
更新日期:1990-07-30 00:00:00
abstract::Glycosylphosphatidylinositol (GPI) is synthesized in the endoplasmic reticulum (ER) and added onto proteins to form GPI-anchored proteins. Among the many proteins involved in this process, ACAT-related enzyme-2 required for viability 1 (Arv1) is a candidate, functioning as a flippase that translocates GPI intermediate...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1002/1873-3468.13843
更新日期:2020-08-01 00:00:00
abstract::PCR-coupled cDNA subtraction hybridization was adapted to identify the genes expressed in the adrenocortical tissues from high salt diet-treated rat. A novel cDNA clone, termed salt-inducible kinase (SIK), encoding a polypeptide (776 amino acids) with significant similarity to protein serine/ threonine kinases in the ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00708-5
更新日期:1999-06-18 00:00:00
abstract::Little is known about the mechanisms underlying calcium-induced Ca2+ release (CICR) in plants. The slow-activating vacuolar (SV) channel is both permeable to, and activated by Ca2+, and is therefore a prime candidate for a role in CICR. Cytosol-side-out vacuolar membrane vesicles loaded with 45Ca2+ showed voltage- and...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)01109-6
更新日期:1999-09-10 00:00:00
abstract::Using specific monoclonal and polyclonal antibodies, we have analyzed protein kinase C alpha and beta isozyme expression in human T cells from peripheral blood (PB) and from thymus. While the PKC beta isozyme was present in all T cell sub-types isolated from both PB and thymus, the alpha isozyme was found only in sing...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)80295-5
更新日期:1994-02-07 00:00:00
abstract::Covalent, mechanism-based inhibitors of glycosidases are valuable probe molecules for visualizing enzyme activities in complex systems. We, here, describe the chemoenzymatic synthesis of 6-phospho-cyclophellitol and evaluate its behaviour as a mechanism-based inactivator of the Streptococcus pyogenes 6-phospho-β-gluco...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1002/1873-3468.12059
更新日期:2016-02-01 00:00:00