Abstract:
:Extracellular vesicles (EVs) contain microRNAs (miRNAs). However, the exact molecular mechanisms of the recruitment of miRNAs in EVs are not well characterized. Based on proteomic analysis, we identified that silencing of Annexin A2 (ANXA2) significantly decreased the amount of miRNAs in EVs. In addition, microarray analysis revealed that ANXA2 regulated the loading of miRNAs into EVs in a sequence independent manner. Lastly, immunoprecipitation analysis confirmed that ANXA2 could bind miRNAs in EVs in the presence of Ca(2+). These observations demonstrate that ANXA2 plays an important role in the packaging process of miRNAs into EVs.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Hagiwara K,Katsuda T,Gailhouste L,Kosaka N,Ochiya Tdoi
10.1016/j.febslet.2015.11.036subject
Has Abstractpub_date
2015-12-21 00:00:00pages
4071-8issue
24 Pt Beissn
0014-5793issn
1873-3468pii
S0014-5793(15)01049-2journal_volume
589pub_type
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