Identification and structural characterization of a mannose-6-phosphate containing oligomannosidic N-glycan from human erythropoietin secreted by recombinant BHK-21 cells.

abstract：:LIM-homeodomain (LIM-HD) proteins are evolutionary conserved developmental transcription factors. LIM-HD Lmx1a and Lmx1b orchestrate complex temporal and spatial gene expression of the dopaminergic pathway, and evidence shows they are also involved in adult neuronal homeostasis. In this review, the multiple roles play...

journal_title：FEBS letters

authors： Doucet-Beaupré H,Ang SL,Lévesque M

更新日期：2015-12-21 00:00:00

abstract：:Epidemiological studies testing the effect of beta-carotene in humans have found a relative risk for lung cancer in smokers supplemented with beta-carotene. We investigated the reactions of retinal and beta-apo-8'-carotenal, two beta-carotene oxidation products, with 2'-deoxyguanosine to evaluate their DNA damaging po...

journal_title：FEBS letters

authors： Marques SA,Loureiro AP,Gomes OF,Garcia CC,Di Mascio P,Medeiros MH

更新日期：2004-02-27 00:00:00

abstract：:Thymidine kinase in chick embryo retina reaches its highest values on the 8-10th day of development, then declines reaching the lowest value at hatching. The rate of DNA synthesis essentially follows this activity while, in contrast, nucleoside phosphotransferase increases progressively during development. Glucocortic...

journal_title：FEBS letters

authors： Tesoriere G,Tesoriere L,Giuliano M,Vento R

更新日期：1983-11-28 00:00:00

abstract：:The present study investigated the mechanism by which eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) inhibit platelet activation induced by thromboxane A2. DHA was found to be more potent than EPA in blocking platelet aggregation induced by the stable thromboxane A2 mimetic, U46619. Furthermore, this inhib...

journal_title：FEBS letters

authors： Swann PG,Venton DL,Le Breton GC

更新日期：1989-01-30 00:00:00

abstract：:During the course of large scale purification of the D1 dopamine receptor from rat brain, a protein of approximately 87,000 daltons (p87) was observed to copurify with the D1 receptor through four chromatographic steps. To characterize the nature of this protein, bovine and rat cDNA clones were isolated and sequenced....

journal_title：FEBS letters

authors： Gingrich JA,Andersen PH,Tiberi M,el Mestikawy S,Jorgensen PN,Fremeau RT Jr,Caron MG

更新日期：1992-11-09 00:00:00

abstract：:Cyclic AMP-mediated phosphorylation of calcium channel subunits was studied in vitro and in vivo in preparations from dog heart. Calcium channels in native cardiac membranes were phosphorylated by cAMP-dependent protein kinase (PKA) solubilized with digitonin and subsequently immunoprecipitated using a polyclonal anti...

journal_title：FEBS letters

authors： Haase H,Karczewski P,Beckert R,Krause EG

更新日期：1993-12-06 00:00:00

abstract：:A synthetic peptide, KKKKRFSFKKSFKLSGFSFKK, containing the phosphorylation sites of the acidic 80-87 kDa protein kinase C substrate was used to identify phosphopeptides in enzyme digests of this protein from mouse fibroblast C3H/10T1/2 cells. Stimulation of phosphorylation occurred, in vivo, with TPA at Ser7, Ser11 an...

journal_title：FEBS letters

authors： Amess B,Manjarrez-Hernandez HA,Howell SA,Learmonth M,Aitken A

更新日期：1992-02-10 00:00:00

abstract：:Comparison of respiration-driven active transport of alkali cations from E. coli cells loaded with Na+ or Li+ showed that Li+ could not be expelled from the cells like Na+. K+ accumulation, which was fast in Na+-loaded cells, was strongly inhibited in Li+-loaded cells, despite high membrane potential and respiratory r...

journal_title：FEBS letters

authors： Verkhovskaya ML,Verkhovsky MI,Wikström M

更新日期：1996-06-17 00:00:00

abstract：:The WW domain is known as one of the smallest protein modules with a triple-stranded beta-sheet fold. Here, we present the solution structure of the second WW domain from the mouse salvador homolog 1 protein. This WW domain forms a homodimer with a beta-clam-like motif, as evidenced by size exclusion chromatography, a...

journal_title：FEBS letters

authors： Ohnishi S,Güntert P,Koshiba S,Tomizawa T,Akasaka R,Tochio N,Sato M,Inoue M,Harada T,Watanabe S,Tanaka A,Shirouzu M,Kigawa T,Yokoyama S

更新日期：2007-02-06 00:00:00

abstract：:Mutations in retinol dehydrogenase 12 (RDH12) cause severe retinal degeneration. However, some of the disease-associated RDH12 mutants retain significant catalytic activity, indicating the existence of additional pathophysiological mechanisms. This study demonstrates that the catalytically active T49M and I51N mutants...

journal_title：FEBS letters

authors： Lee SA,Belyaeva OV,Kedishvili NY

更新日期：2010-02-05 00:00:00

abstract：:Human natural killer (NK) cells possess an arsenal of receptors programmed to regulate the NK cell functions, once encountering a target cell. In general, the activating receptors mediate cytotoxicity when engaged by their tumor specific, stress induced, virally encoded, or rarely, self ligands. Whereas, the inhibitor...

journal_title：FEBS letters

authors： Stanietsky N,Mandelboim O

更新日期：2010-12-15 00:00:00

abstract：:1,2-sn-Dihexanoylglycerol (HHG) reduced the ATP content of HL-60 cells. This concentration-related (10-100 microM) effect reached a maximum of over 90%, was enantiomerically specific and not accompanied by release of lactate dehydrogenase. Oleoylacetylglycerols (3-100 microM) had no effect on ATP levels while phorbol ...

journal_title：FEBS letters

authors： Dawson J,Thompson NT,Bonser RW,Hodson HF,Garland LG

更新日期：1987-04-06 00:00:00

abstract：:Cloned mouse delta-subtype opioid receptor (DOR1) was expressed in Xenopus oocytes to study the signal transduction. Opioid delta-agonists evoked a calcium-dependent chloride current in oocytes injected with mRNA derived from DOR1, together with that from the alpha subunit of Gi1. The delta-agonist-induced current was...

journal_title：FEBS letters

authors： Miyamae T,Fukushima N,Misu Y,Ueda H

更新日期：1993-11-01 00:00:00

abstract：:A variant form of the heptacosapeptide amide secretin, with C-terminal -Val-Gly-Lys-Arg instead of valine amide, has been isolated from porcine upper intestinal tissue. Unexpectedly, this triacontapeptide exhibited a substantially higher bioactivity than the heptacosapeptide amide. ...

journal_title：FEBS letters

authors： Gafvelin G,Carlquist M,Mutt V

更新日期：1985-05-20 00:00:00

abstract：:Michaelis-Menten kinetics of glycerol-3-phosphate dehydrogenase activity in proteoliposomes from brown adipose tissue mitochondria with exogenously added phospholipids or cholesterol was measured. It was shown that changes in membrane lipid composition affected the membrane lateral pressure and therefore modulated the...

journal_title：FEBS letters

authors： Amler E,Jasińska R,Drahota Z,Zborowski J

更新日期：1990-10-01 00:00:00

abstract：:In addition to the classical functions of the Golgi in membrane transport and glycosylation, the Golgi apparatus of mammalian cells is now recognised to contribute to the regulation of a range of cellular processes, including mitosis, DNA repair, stress responses, autophagy, apoptosis and inflammation. These processes...

journal_title：FEBS letters

authors： Kulkarni-Gosavi P,Makhoul C,Gleeson PA

更新日期：2019-09-01 00:00:00

abstract：:Large conductance voltage-dependent and Ca(2+)-modulated K(+) channels play a crucial role in myometrium contractility. Western blots and immunocytochemistry of rat uterine sections or isolated cells show that MaxiK channel protein signals drastically decrease towards the end of pregnancy. Consistent with a transcript...

journal_title：FEBS letters

authors： Song M,Zhu N,Olcese R,Barila B,Toro L,Stefani E

更新日期：1999-11-05 00:00:00

abstract：:Endoplasmic reticulum stress (ER-stress) is associated with ataxia telangiectasia mutated (ATM) gene. We present here conclusive data showing that ATM blocks ER-stress induced by tunicamycin or ionizing radiation (IR). X-box protein-1 (XBP-1) splicing, GRP78 expression and caspase-12 activation were increased by tunic...

journal_title：FEBS letters

authors： He L,Kim SO,Kwon O,Jeong SJ,Kim MS,Lee HG,Osada H,Jung M,Ahn JS,Kim BY

更新日期：2009-03-04 00:00:00

abstract：:The nuclear magnetic resonance solution structure of alpha-conotoxin SI has been determined at pH 4.2. The 36 lowest energy structures show that alpha-conotoxin SI exists in a single major solution conformation and is stabilized by six hydrogen bonds. Comparisons are made between the SI solution structure and the solu...

journal_title：FEBS letters

authors： Benie AJ,Whitford D,Hargittai B,Barany G,Janes RW

更新日期：2000-07-07 00:00:00

abstract：:In addition to their g = 1.94 EPR signal, nitrogenase Fe-proteins from Azotobacter vinelandii, Azotobacter chroococcum and Klebsiella pneumoniae exhibit a weak EPR signal with g approximately equal to 5. Temperature dependence of the signal was consistent with an S = 3/2 system with negative zero-field splitting, D = ...

journal_title：FEBS letters

authors： Hagen WR,Eady RR,Dunham WR,Haaker H

更新日期：1985-09-23 00:00:00

abstract：:The effect of caffeine on catecholamine secretion and intracellular free Ca2+ concentration [( Ca2+]i) in bovine adrenal chromaffin cells was examined using single fura-2-loaded cells and cell populations. In cell populations caffeine elicited a large (approximately 200 nM) transient rise in [Ca2+]i that was independe...

journal_title：FEBS letters

authors： Cheek TR,O'Sullivan AJ,Moreton RB,Berridge MJ,Burgoyne RD

更新日期：1990-06-18 00:00:00

abstract：:The photochemical reaction center of Chloroflexus (Cf.) aurantiacus, a membrane bound pigment-protein complex, has been crystallized in the presence of monodisperse polyoxyethylene detergents. The crystals possessed a pronounced polymorphism. Three different crystal forms belonging to triclinic, monoclinic and orthorh...

journal_title：FEBS letters

authors： Feick R,Ertlmaier A,Ermler U

更新日期：1996-11-04 00:00:00

abstract：:The actin-depolymerising factor (ADF)/cofilin group of proteins are stimulus-responsive actin-severing proteins, members of which are regulated by reversible phosphorylation. The phosphorylation site on the maize ADF, ZmADF3, is Ser-6 but the kinase responsible is unknown [Smertenko et al., Plant J. 14 (1998) 187-193]...

journal_title：FEBS letters

authors： Allwood EG,Smertenko AP,Hussey PJ

更新日期：2001-06-15 00:00:00

abstract：:A cytosolic calmodulin wave has been described in the pre-replicative phase of rat liver regeneration. Here we demonstrate that this calmodulin increase is reduced by injection of cycloheximide (translation inhibitor) and actinomycin D (transcription inhibitor). In addition, we found that calmodulin mRNA levels are in...

journal_title：FEBS letters

authors： Piõl MR,Berchtold MW,Bachs O,Heizmann CW

更新日期：1988-04-25 00:00:00

abstract：:Factor VIIa is a vitamin K-dependent enzyme whose gamma-carboxyglutamic acid (Gla)-containing domain is important for calcium ion-dependent binding to the cofactor tissue factor and membrane surfaces. This domain contains 10 Gla residues, the individual roles and importance of which are not known. Comparisons with the...

journal_title：FEBS letters

authors： Persson E,Nielsen LS

更新日期：1996-05-06 00:00:00

abstract：:For almost 30 years, Wnt proteins have been known as key regulators of many developmental decisions, including the formation of the embryonic axes, patterning of the CNS, limb bud outgrowth and segment polarity. However, live cell imaging of active Wnt proteins was rarely reported. Here, we have generated a Wnt2b-EGFP...

journal_title：FEBS letters

authors： Holzer T,Liffers K,Rahm K,Trageser B,Ozbek S,Gradl D

更新日期：2012-06-04 00:00:00

abstract：:Human ECE-1 is expressed in four isoforms with different tissue distribution and its mRNA and protein levels are altered under certain pathophysiological conditions. To investigate the transcriptional regulation of ECE-1, we studied the regulatory region of ECE-1c, the major ECE-1 isoform. A genomic clone comprising t...

journal_title：FEBS letters

authors： Funke-Kaiser H,Bolbrinker J,Theis S,Lemmer J,Richter CM,Paul M,Orzechowski HD

更新日期：2000-01-28 00:00:00

abstract：:The Escherichia coli twin-arginine translocation (Tat) system serves to export fully folded protein substrates across the bacterial cytoplasmic membrane. Respiratory [NiFe] hydrogenases are synthesised as precursors with twin-arginine signal peptides and transported as large, cofactor-containing, multi-subunit complex...

journal_title：FEBS letters

authors： Dubini A,Sargent F

更新日期：2003-08-14 00:00:00

abstract：:The GAL11 gene encodes an auxiliary transcription factor required for full expression of many, if not all, genes of the yeast Saccharomyces cerevisiae. We have recently shown that GAL11-encoded protein (Gal11p) enhances basal transcription from the CYC1 promoter in a cell-free transcription system [(1993) Proc. Natl. ...

journal_title：FEBS letters

authors： Sakurai H,Ohishi T,Amakasu H,Fukasawa T

更新日期：1994-09-05 00:00:00

abstract：:The binding of Mg2+ ADP to both rabbit skeletal and bovine cardiac myofibrils has been studied at two different temperatures. In each case a single class of binding sites was observed with a binding constant very close to that reported for the analogous actomyosin-subfragment one but much weaker than that seen with th...

journal_title：FEBS letters

authors： Johnson RE,Adams PH

更新日期：1984-08-20 00:00:00