Analysis of U1snRNP-specific A protein cross-linked complexes.

Abstract:

:The organization of the U1snRNP-specific A protein (34 kDa) has been analyzed by 12 and 16 A thiol-reversible chemical cross-linking and Western blotting. A-containing cross-linked complexes had molecular masses of 43, 47, 56, 62, 67, 105 and 125 kDa. None of these complexes could be cross-linked following ribonuclease digestion, suggesting that UsnRNA may play important roles in the spatial organization of A and other proteins. Moreover, the data suggest that A is proximal to, and may have interactions with, UsnRNP-specific proteins C and 70 kDa as well as with UsnRNP-common proteins B, E and G.

journal_name

FEBS Lett

journal_title

FEBS letters

authors

Boix J,Habets WJ,van Venrooij WJ,Smith HC

doi

10.1016/0014-5793(90)80487-4

subject

Has Abstract

pub_date

1990-10-15 00:00:00

pages

213-6

issue

1-2

eissn

0014-5793

issn

1873-3468

pii

0014-5793(90)80487-4

journal_volume

272

pub_type

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