Abstract:
:An antibody recognising acetylated core histones was used to immunoprecipitate chromatin fragments from proliferating human K562 cells and from cells induced to differentiate with the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA). The DNA of the acetylated chromatin was probed with sequences of platelet derived growth factor B chain (PDGF-B), a gene which is induced to strong expression upon differentiation. A high level of acetylation was observed before gene induction and no change seen following induction. This implies that core histone acetylation is an essential precondition for transcription.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Clayton AL,Hebbes TR,Thorne AW,Crane-Robinson Cdoi
10.1016/0014-5793(93)81601-usubject
Has Abstractpub_date
1993-12-20 00:00:00pages
23-6issue
1eissn
0014-5793issn
1873-3468pii
0014-5793(93)81601-Ujournal_volume
336pub_type
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