Abstract:
:It was believed that there was no natural suppressor tRNA in Escherichia coli, however, it has been suggested that selC, relating to the synthesis of formate dehydrogenase of a selenoprotein [(1988) Nature 331, 723-725], codes for tRNA, even though the presence of tRNA has not yet been demonstrated. We detected the product of selC in the tRNA preparation of the E. coli MC 4100 strain by the dot blot hybridization method with a DNA probe (ACCGCTGGCGGC) corresponding to the extra arm of selC tRNA. Two hybridization peaks were found in the chromatographic pattern from Sephadex A50. The amount of tRNA was estimated to be about 0.03% of the total tRNA. Some tRNA [corrected] was phosphorylated by a tRNA kinase in E. coli B. These results suggest that the opal suppressor seryl-tRNA in E. coli should be converted to selenocysteyl-tRNA [corrected] and occurs in vertebrates as a general phenomenon.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Mizutani T,Maruyama N,Hitaka T,Sukenaga Ydoi
10.1016/0014-5793(89)81367-5subject
Has Abstractpub_date
1989-04-24 00:00:00pages
345-8issue
2eissn
0014-5793issn
1873-3468journal_volume
247pub_type
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