Active transport of lead by human red blood cells.


:Human red cells suspended in lead-citrate buffers (2.6 microM Pb2+) take up much less Pb than predicted from studies of equilibrium binding of Pb to haemolysates. Pb uptake is increased by ATP depletion, or by loading at 0 degrees C. Tracer studies with 203Pb indicate that the low uptake at 37 degrees C in the presence of substrate is not due to membrane impermeability to Pb. Cold-loaded cells extrude Pb against a concentration gradient at 37 degrees C when glucose is present. These results suggest that the cellular loading of Pb is dependent on the balance between an inward leak and an outward pump. The extrusion of Pb from the cells is possibly brought by the Ca pump.




FEBS letters


Simons TJ




Has Abstract


1984-07-09 00:00:00














  • A common evolutionary origin of two elementary enzyme folds.

    abstract::The (beta alpha)(8)-barrel is the most frequent and most versatile fold among enzymes [Höcker et al., Curr. Opin. Biotechnol. 12 (2001) 376-381; Wierenga, FEBS Lett. 492 (2001) 193-198]. Structural and functional evidence suggests that (beta alpha)(8)-barrels evolved from an ancestral half-barrel, which consisted of f...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Höcker B,Schmidt S,Sterner R

    更新日期:2002-01-16 00:00:00

  • Modulation of cardiac Ca2+ channels in Xenopus oocytes by protein kinase C.

    abstract::L-Type calcium channel was expressed in Xenopus laevis oocytes injected with RNAs coding for different cardiac Ca2+ channel subunits, or with total heart RNA. The effects of activation of protein kinase C (PKC) by the phorbol ester PMA (4 beta-phorbol 12-myristate 13-acetate) were studied. Currents through channels co...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Singer-Lahat D,Gershon E,Lotan I,Hullin R,Biel M,Flockerzi V,Hofmann F,Dascal N

    更新日期:1992-07-20 00:00:00

  • Tyrosine phosphorylation of HPK1 by activated Src promotes ischemic brain injury in rat hippocampal CA1 region.

    abstract::Hematopoietic progenitor kinase 1 (HPK1) is a hematopoietic cell-restricted member of the Ste20 serine/threonine kinase super family. We recently reported that HPK1 is involved in c-Jun NH2-terminal kinase (JNK) signaling pathway by sequential activation of MLK3-MKK7-JNK3 after cerebral ischemia. Here, we used 4-amino...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Li T,Yu XJ,Zhang GY

    更新日期:2008-06-11 00:00:00

  • Gene duplication in bovine brain myelin proteolipid and homology with related proteins.

    abstract::Analysis of the amino acid sequence of bovine brain myelin proteolipid reveals not only extensive internal homology, but also homology with portions of the myelin basic protein, the peripheral nerve myelin protein, Po, and with the small proteolipid subunit of mitochondrial ATP synthase. These results suggest that the...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Laursen RA,Samiullah M,Lees MB

    更新日期:1983-09-05 00:00:00

  • Translation arrest as a protein quality control system for aberrant translation of the 3'-UTR in mammalian cells.

    abstract::Read-through or mutations of a stop codon resulting in translation of the 3'-UTR produce potentially toxic C-terminally extended proteins. However, quality control mechanisms for such proteins are poorly understood in mammalian cells. Here, a comprehensive analysis of the 3'-UTRs of genes associated with hereditary di...

    journal_title:FEBS letters

    pub_type: 社论


    authors: Hashimoto S,Nobuta R,Izawa T,Inada T

    更新日期:2019-04-01 00:00:00

  • In various protein complexes, disordered protomers have large per-residue surface areas and area of protein-, DNA- and RNA-binding interfaces.

    abstract::We provide first large scale analysis of the peculiarities of surface areas of 5658 dissimilar (below 50% sequence similarity) proteins with known 3D-structures that bind to proteins, DNA or RNAs. We show here that area of the protein surface is highly correlated with the protein length. The size of the interface surf...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Wu Z,Hu G,Yang J,Peng Z,Uversky VN,Kurgan L

    更新日期:2015-09-14 00:00:00

  • Cloning, expression and characterization of a novel four EF-hand Ca(2+)-binding protein from olive pollen with allergenic activity.

    abstract::A novel allergenic member of the family of Ca(2+)-binding proteins has been cloned from olive tree pollen. The isolated DNA codes for a protein of 171 amino acid residues, which displays four EF-hand sequence motifs. The encoded protein was overproduced in Escherichia coli and purified. The protein (18¿ omitted¿795 Da...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Ledesma A,Villalba M,Rodríguez R

    更新日期:2000-01-21 00:00:00

  • The hexose carrier from Chlorella. cDNA cloning of a eucaryotic H+-cotransporter.

    abstract::The cDNA coding for the inducible H+/hexose cotransporter of Chlorella kessleri has been cloned and sequenced. It was isolated by differential screening of a cDNA library prepared from glucose-induced cells. The increase in expression of the gene correlates quantitatively with the increase in uptake activity due to in...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Sauer N,Tanner W

    更新日期:1989-12-18 00:00:00

  • Membrane lipid composition protects Entamoeba histolytica from self-destruction by its pore-forming toxins.

    abstract::The protozoan parasite and human pathogen Entamoeba histolytica is protected against killing by its own lytic effector proteins. Amoebae withstand doses of amoebapores, their pore-forming polypeptides, that readily kill human Jurkat T cells. Moreover, the polypeptides do not bind to the amoebic surface membrane as evi...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Andrä J,Berninghausen O,Leippe M

    更新日期:2004-04-23 00:00:00

  • Conformational responses of an arachidonate- and U46619-binding haemoprotein in relation to platelet activation.

    abstract::Antibodies were raised, in rabbits, against an arachidonate- and U46619-binding protein purified from calf platelets. Spectral measurements and immunodiffusion experiments were employed to follow conformational responses of the protein in relation to platelet activation. Upon treatment with the platelet agonists, arac...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Krishnan LK,Jamaluddin MP

    更新日期:1987-02-23 00:00:00

  • Polysulfide exerts a protective effect against cytotoxicity caused by t-buthylhydroperoxide through Nrf2 signaling in neuroblastoma cells.

    abstract::Polysulfide is a bound sulfur species derived from endogenous H2S. When mouse neuroblastoma, Neuro2A cells were exposed to tert-butyl hydroperoxide after treatment with polysulfide, a significant decline in cell toxicity was observed. Rapid uptake of polysulfides induced translocation of Nrf2 into the nucleus, resulti...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Koike S,Ogasawara Y,Shibuya N,Kimura H,Ishii K

    更新日期:2013-11-01 00:00:00

  • Characterization of an Arabidopsis thaliana mutant with impaired psbO, one of two genes encoding extrinsic 33-kDa proteins in photosystem II.

    abstract::A 33-kDa protein component of the oxygen-evolving complex in photosystem II is essential for photosynthesis, and it has been believed that mutants with deletion of this 33-kDa protein are not found in higher plants. We report here the first isolation of an Arabidopsis thaliana mutant with a defect in one of the genes ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Murakami R,Ifuku K,Takabayashi A,Shikanai T,Endo T,Sato F

    更新日期:2002-07-17 00:00:00

  • ADP-ribosylation of nuclear proteins is increased by phenobarbital. Identification of the ADP-ribosylated histone fractions in rat liver nuclei.

    abstract::Changes in the ADP-ribosylation of total proteins and purified histones of rat liver nuclei after phenobarbital treatment (80 mg/kg, 24 h) have been studied. The [32P]NAD incorporation into total trichloroacetic acid precipitated proteins, in histone Hl and in core histones was evaluated, the specific radioactivities ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Bràz J,Lechner MC

    更新日期:1986-04-21 00:00:00

  • Crystal structure of AmyA lacks acidic surface and provide insights into protein stability at poly-extreme condition.

    abstract::Here we report the first crystal structure of a protein, AmyA, a secretory alpha-amylase isolated from Halothermothrix orenii, which is both halophilic and thermophilic. The crystal structure was determined at 1.6 A resolution. AmyA lacks the conserved acidic surface, which is considered essential for protein stabilit...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Sivakumar N,Li N,Tang JW,Patel BK,Swaminathan K

    更新日期:2006-05-15 00:00:00

  • Continuous hepatocyte growth factor supply prevents lipopolysaccharide-induced liver injury in rats.

    abstract::We present a rat model in which continuous supply of hepatocyte growth factor (HGF) prevents liver injury induced by carbon tetrachloride (CCl4) and E. coli 011:B4 lipopolysaccharide (LPS). Rat fibroblasts genetically modified to secrete rat HGF were implanted in syngenic rat spleen 7 days before administration of the...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kaido T,Yamaoka S,Seto S,Funaki N,Kasamatsu T,Tanaka J,Nakamura T,Imamura M

    更新日期:1997-07-14 00:00:00

  • Sequence homologies among mitochondrial DNA-coded URF2, URF4 and URF5.

    abstract::Amino acid sequences coded for by 13 different genes of mammalian mitochondrial DNA (mtDNA) including 8 unassigned open reading frames (URFs) were compared in pairs. It was found that significant homologies exist among the amino acid sequences of the three URFs (URF2, URF4 and URF5) with a probability of occurrence of...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kikuno R,Miyata T

    更新日期:1985-09-09 00:00:00

  • Expression of an inwardly rectifying K+ channel from rat basophilic leukemia cell mRNA in Xenopus oocytes.

    abstract::Rat basophilic leukemia cells (RBL-2H3) have previously been shown to contain a single type of voltage-activated channel, namely an inwardly rectifying K+ channel, under normal recording conditions. Thus, RBL-2H3 cells seemed like a logical source of mRNA for the expression cloning of inwardly rectifying K+ channels. ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Lewis DL,Ikeda SR,Aryee D,Joho RH

    更新日期:1991-09-23 00:00:00

  • Biochemical identification of the catalytic residues of a glycoside hydrolase family 120 β-xylosidase, involved in xylooligosaccharide metabolisation by gut bacteria.

    abstract::The β-xylosidase B from Bifidobacterium adolescentis ATCC15703 belongs to the newly characterized family 120 of glycoside hydrolases. In order to investigate its catalytic mechanism, an extensive kinetic study of the wild-type enzyme and mutants targeting the three highly conserved residues Asp(393), Glu(416) and Glu(...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Cecchini DA,Fauré R,Laville E,Potocki-Veronese G

    更新日期:2015-10-07 00:00:00

  • Charybdotoxin blocks both Ca-activated K channels and Ca-independent voltage-gated K channels in rat brain synaptosomes.

    abstract::Charybdotoxin (ChTX), a 4.3 kDa polypeptide toxin from the venom of the scorpion Leiurus quinquestriatus, blocks both a Ca-activated K channel (IC50 approximately 15 nM) and a Ca-independent voltage-gated K channel (IC50 approximately 40 nM) in rat brain synaptosomes. These results indicate that in this preparation Ch...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Schneider MJ,Rogowski RS,Krueger BK,Blaustein MP

    更新日期:1989-07-03 00:00:00

  • Complexes between 15 kDa caldesmon fragment and actin investigated by immuno-electron microscopy.

    abstract::The regulatory system of smooth muscle thin filaments is thought to involve a major calcium-calmodulin and actin binding protein: caldesmon. A dissective approach was used to isolate a 35 kDa C-terminal fragment of the molecule and to produce antibodies reacting against both the intact and the 15 kDa N-terminal end of...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Harricane MC,Cavadore C,Audemard E,Mornet D

    更新日期:1990-08-20 00:00:00

  • Electrostatic influence of active-site waters on the nucleophilic aromatic substitution catalyzed by 4-chlorobenzoyl-CoA dehalogenase.

    abstract::The 4-chlorobenzoyl-CoA dehalogenase catalyzes the hydrolytic dechlorination of 4-chlorobenzoyl-CoA via a two-step mechanism, namely nucleophilic aromatic substitution and ester hydrolysis. The mutation of an active-site Histidine residue has been shown to reduce the catalytic activity in both the substitution and sub...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Xu D,Guo H

    更新日期:2005-08-15 00:00:00

  • FITC-labeled lipopolysaccharide: use as a probe for liposomal membrane incorporation studies.

    abstract::FITC-labeled LPS from Neisseria meningitidis can be used as a probe to follow the process of LPS incorporation into liposomal membrane and to study its interaction with a bilayer. The incorporation of FITC-LPS into the bilayer was proved by physicochemical methods as well as by liposomal LPS toxicity decrease in actin...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Trubetskoy VS,Koshkina NV,Omel'yanenko VG,L'vov VL,Dmitriev BA,Petrov AB,Torchilin VP

    更新日期:1990-08-20 00:00:00

  • Mis-annotations of a promising antibiotic target in high-priority gram-negative pathogens.

    abstract::The rise of antibiotic resistance combined with the lack of new products entering the market has led to bacterial infections becoming one of the biggest threats to global health. Therefore, there is an urgent need to identify novel antibiotic targets, such as dihydrodipicolinate synthase (DHDPS), an enzyme involved in...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Impey RE,Lee M,Hawkins DA,Sutton JM,Panjikar S,Perugini MA,Soares da Costa TP

    更新日期:2020-05-01 00:00:00

  • Measurement of GTP gamma S binding to specific G proteins in membranes using G-protein antibodies.

    abstract::We developed a novel method to quantitatively measure GTP gamma S binding to specific G proteins in crude membranes using G-protein antibodies. The basic strategy was that the materials were initially incubated with [35S]GTP gamma S at 37 degrees C. After 4 degrees C incubation in the wells of an ELISA plate precoated...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Okamoto T,Ikezu T,Murayama Y,Ogata E,Nishimoto I

    更新日期:1992-06-29 00:00:00

  • TUCAN/CARDINAL and DRAL participate in a common pathway for modulation of NF-kappaB activation.

    abstract::Proteins containing the caspase recruiting domain (CARD) have emerged as critical regulators of different signal transduction pathways, including those controlling apoptosis and activation of necrosis factor (NF)-kappaB transcription factor. TUCAN/CARDINAL is a recently identified CARD-containing protein involved in r...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Stilo R,Leonardi A,Formisano L,Di Jeso B,Vito P,Liguoro D

    更新日期:2002-06-19 00:00:00

  • Melatonin elevates intracellular free calcium in human platelets by inositol 1,4,5-trisphosphate independent mechanism.

    abstract::Several studies have indicated the existence of direct effects of melatonin on platelets. Here we show that, melatonin at high concentration is capable of significantly raising platelet intracellular calcium even in the absence of an agonist. The effect of melatonin on platelets was abolished by luzindole, a melatonin...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kumari S,Dash D

    更新日期:2011-07-21 00:00:00

  • Glucosyl epi-cyclophellitol allows mechanism-based inactivation and structural analysis of human pancreatic α-amylase.

    abstract::As part of a search for selective, mechanism-based covalent inhibitors of human pancreatic α-amylase we describe the chemoenzymatic synthesis of the disaccharide analog α-glucosyl epi-cyclophellitol, demonstrate its stoichiometric reaction with human pancreatic α-amylase and evaluate the time dependence of its inhibit...

    journal_title:FEBS letters

    pub_type: 信件


    authors: Caner S,Zhang X,Jiang J,Chen HM,Nguyen NT,Overkleeft H,Brayer GD,Withers SG

    更新日期:2016-04-01 00:00:00

  • Voltage-gated proton (H(v)1) channels, a singular voltage sensing domain.

    abstract::The main role of voltage-gated proton channels (Hv1) is to extrude protons from the intracellular milieu when, mediated by different cellular processes, the H(+) concentration increases. Hv1 are exquisitely selective for protons and their structure is homologous to the voltage sensing domain (VSD) of other voltage-gat...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Castillo K,Pupo A,Baez-Nieto D,Contreras GF,Morera FJ,Neely A,Latorre R,Gonzalez C

    更新日期:2015-11-14 00:00:00

  • Chloride channels of intracellular membranes.

    abstract::Proteins implicated as intracellular chloride channels include the intracellular ClC proteins, the bestrophins, the cystic fibrosis transmembrane conductance regulator, the CLICs, and the recently described Golgi pH regulator. This paper examines current hypotheses regarding roles of intracellular chloride channels an...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Edwards JC,Kahl CR

    更新日期:2010-05-17 00:00:00

  • Insulin activates protein kinase B, inhibits glycogen synthase kinase-3 and activates glycogen synthase by rapamycin-insensitive pathways in skeletal muscle and adipose tissue.

    abstract::Insulin stimulated protein kinase B alpha (PKB alpha) more than 10-fold and decreased glycogen synthase kinase-3 (GSK3) activity by 50 +/- 10% in skeletal muscle and adipocytes. Rapamycin did not prevent the activation of PKB, inhibition of GSK3 or stimulation of glycogen synthase up to 5 min. Thus rapamycin-insensiti...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Cross DA,Watt PW,Shaw M,van der Kaay J,Downes CP,Holder JC,Cohen P

    更新日期:1997-04-07 00:00:00