Abstract:
:Human red cells suspended in lead-citrate buffers (2.6 microM Pb2+) take up much less Pb than predicted from studies of equilibrium binding of Pb to haemolysates. Pb uptake is increased by ATP depletion, or by loading at 0 degrees C. Tracer studies with 203Pb indicate that the low uptake at 37 degrees C in the presence of substrate is not due to membrane impermeability to Pb. Cold-loaded cells extrude Pb against a concentration gradient at 37 degrees C when glucose is present. These results suggest that the cellular loading of Pb is dependent on the balance between an inward leak and an outward pump. The extrusion of Pb from the cells is possibly brought by the Ca pump.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Simons TJdoi
10.1016/0014-5793(84)81135-7subject
Has Abstractpub_date
1984-07-09 00:00:00pages
250-4issue
2eissn
0014-5793issn
1873-3468pii
0014-5793(84)81135-7journal_volume
172pub_type
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