Abstract:
:We transfected the COS-7 cells with cDNAs encoding different human somatostatin receptor (hSSTR) subtypes, and found that hSSTR subtypes mediate not only the inhibition of forskolin-induced cAMP accumulation but also the stimulation of phospholipase C (PLC) and Ca2+ mobilization. Activation of PLC by 1 microM somatostatin (SRIF) was in the order of: hSSTR5 > hSSTR2 > hSSTR3 > hSSTR4 > hSSTR1. Pertussis toxin (PTX) treatment completely or partially reversed the PLC activation. 1 nM SRIF was equally effective for adenylate cyclase (AC) inhibition in a PTX-sensitive manner, in all the cells expressing different hSSTRs, except for hSSTR1. Nevertheless, SRIF stimulated AC even in the presence of forskolin at higher doses of SRIF in PTX-treated hSSTR5-expressing cells. We conclude that the cloned hSSTRs differentially couple to PTX-sensitive and -insensitive G-proteins to modulate PLC, Ca2+ mobilization and AC.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Akbar M,Okajima F,Tomura H,Majid MA,Yamada Y,Seino S,Kondo Ydoi
10.1016/0014-5793(94)00603-2subject
Has Abstractpub_date
1994-07-11 00:00:00pages
192-6issue
2eissn
0014-5793issn
1873-3468pii
0014-5793(94)00603-2journal_volume
348pub_type
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