Abstract:
:Astrocyte-elevated gene-1 (AEG-1) positively regulates tumor progression and metastasis. Here, we document that AEG-1 upregulates transcription of the membrane protein tetraspanin 8 (TSPAN8). Knocking down TSPAN8 in AEG-1-overexpressing human hepatocellular carcinoma (HCC) cells markedly inhibited invasion and migration without affecting proliferation. TSPAN8 knockdown profoundly abrogated AEG-1-induced primary tumor and intrahepatic metastasis in an orthopic xenograft model in athymic nude mice. Coculture of TSPAN8 knockdown cells with human umbilical vein endothelial cells (HUVEC) markedly inhibited HUVEC tube formation indicating that inhibition of angiogenesis might cause reduction in primary tumor size. TSPAN8 inhibition might be a potential therapeutic strategy for metastatic HCC.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Akiel MA,Santhekadur PK,Mendoza RG,Siddiq A,Fisher PB,Sarkar Ddoi
10.1002/1873-3468.12268subject
Has Abstractpub_date
2016-08-01 00:00:00pages
2700-8issue
16eissn
0014-5793issn
1873-3468journal_volume
590pub_type
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