Abstract:
:Using time-resolved low-temperature spectroscopy, we have examined whether or not bovine rhodopsin has a unique transducin-binding state, meta Ib, previously detected from chicken rhodopsin. Unlike chicken meta Ib, bovine meta Ib was detected only by detailed kinetics analysis of the bleaching process, but it was stabilized by transducin and visualized in the observed spectral changes. From the effect of GTPgammaS, it was revealed that meta Ib induced no GDP-GTP exchange reaction in transducin. Thus meta Ib is a common intermediate of vertebrate rhodopsin and transducin is activated in two steps by meta Ib and meta II.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Tachibanaki S,Imai H,Terakita A,Shichida Ydoi
10.1016/s0014-5793(98)00216-6subject
Has Abstractpub_date
1998-03-20 00:00:00pages
126-30issue
1eissn
0014-5793issn
1873-3468pii
S0014-5793(98)00216-6journal_volume
425pub_type
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