Abstract:
:We have reconstituted thin filaments comprising pyrene-labelled actin (pyr-actin), tropomyosin (Tm) and cardiac troponin (cTn). cTn was isolated in two defined phosphorylation states; completely dephosphorylated on all subunits and with only the cTnI subunit bisphosphorylated. The thin filament was saturated with cTn at a pyr-actin/Tm/cTn ratio of 7:1:1. The calcium-dependent binding of S1 to thin filaments was measured in a stopped-flow spectrophotometer and the dependence of the observed rate constant on [Ca2+] fitted to the Hill equation. The only significant difference between the two phosphorylation states of the filaments was a 0.36 decrease in the pCa50 on bisphosphorylation.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Reiffert SU,Jaquet K,Heilmeyer LM Jr,Ritchie MD,Geeves MAdoi
10.1016/0014-5793(96)00274-8subject
Has Abstractpub_date
1996-04-08 00:00:00pages
43-7issue
1eissn
0014-5793issn
1873-3468pii
0014-5793(96)00274-8journal_volume
384pub_type
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