Abstract:
:Fatty acid synthase (Fasn) is a key component of energy metabolism that is dynamically induced by food intake. Although extensive studies have revealed a number of transcription factors involved in the fasting/refeeding transition of Fasn expression in hepatocytes, much less evidence is available for adipocytes. Using the in vivo Ad-luc analytical system, we identified the inverted CCAAT element (ICE) around -100 nucleotides in the Fasn promoter as a critical cis-element for the refeeding response in adipocytes. Electrophoretic mobility shift assays and chromatin immunoprecipitation show that nuclear factor Y (NF-Y) binds to ICE specifically in refeeding states. Notably, the NF-Y binding to ICE is differently regulated between adipocytes and hepatocytes. These findings provide insights into the specific mechanisms controlling energy metabolism in adipocytes.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Nishi-Tatsumi M,Yahagi N,Takeuchi Y,Toya N,Takarada A,Murayama Y,Aita Y,Sawada Y,Piao X,Oya Y,Shikama A,Masuda Y,Kubota M,Izumida Y,Matsuzaka T,Nakagawa Y,Sekiya M,Iizuka Y,Kawakami Y,Kadowaki T,Yamada N,Shimanodoi
10.1002/1873-3468.12620subject
Has Abstractpub_date
2017-04-01 00:00:00pages
965-978issue
7eissn
0014-5793issn
1873-3468journal_volume
591pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::A human Cu,Zn-superoxide dismutase (Cu,Zn-SOD) gene was fused with a gene fragment encoding the nine amino acid transactivator of transcription (Tat) protein transduction domain (RKKRRQRRR) of HIV-1 in a bacterial expression vector to produce a genetic in-frame Tat-SOD fusion protein. The expressed and purified Tat-SO...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)02215-8
更新日期:2000-11-24 00:00:00
abstract::alpha2-Macroglobulin was derivatized with a sulfhydryl cross-linker and sandwiched between a mica substrate and a silicon nitride tip, both coated with gold, of an atomic force microscope and force curve measurement was carried out. An extensive downward deflection of the cantilever was observed in the retracting real...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)00319-5
更新日期:1996-04-29 00:00:00
abstract::Mammalian voltage-activated Shaker K+ channels associate with at least three cytoplasmic proteins: Kv beta 1, Kv beta 2 and Kv beta 3. These beta subunits contain variable N-termini, which can modulate the inactivation of Shaker alpha subunits, but are homologous throughout an aldo-keto reductase core. Human and ferre...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)00785-8
更新日期:1995-08-14 00:00:00
abstract::Engrailed-2 (En-2) belongs to an evolutionarily conserved family of DNA binding homeodomain-containing proteins that are expressed in mammalian brain during development. Here, we demonstrate that serine 267 in the homeodomain of En-2 is phosphorylated by protein kinase A (PKA) in forskolin-treated COS-7 cells. Further...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2004.05.009
更新日期:2004-06-18 00:00:00
abstract::Increases in choline containing metabolites have been associated with a number of disorders, including malignant cell growth. In this study, high resolution magic angle spinning (1)H nuclear magnetic resonance spectroscopy was employed to monitor metabolite changes during cell transfection, and an increase in phosphoc...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)03175-1
更新日期:2001-12-07 00:00:00
abstract::Eleven cloned hybridomas which secrete antibodies to acetylcholine receptors from human muscle have been prepared. All of these monoclonal antibodies to have the same basic specificity as shown by competition for binding to the main immunogenic region on the receptor, but these antibodies differ in fine specificity as...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)80688-7
更新日期:1983-07-11 00:00:00
abstract::DU-PAN-2 reactive gangliosides were isolated from the tumor of a patient with pancreatic cancer (duct cell carcinoma, moderately differentiated adenocarcinoma), having a negative Lewis blood phenotype, and were analyzed by means of TLC-immunostaining, enzyme-linked immunosorbent assay (ELISA), permethylation study, 1H...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)01004-8
更新日期:1994-10-10 00:00:00
abstract::Plasmids for the high-level overproduction of wild-type, and C- and N-terminal His-tagged MurG N-acetylglucosaminyl transferase from Escherichia coli were constructed. In complementation tests the three forms were active in vivo. After IPTG induction, growth, spheroplast formation and lysis, overproduced MurG proteins...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00412-3
更新日期:1999-04-23 00:00:00
abstract::Friedreich ataxia is the most common recessive neurodegenerative disease and is caused by reduced expression of mitochondrial frataxin. Frataxin depletion causes impairment in iron-sulfur cluster and heme biosynthesis, disruption of iron homeostasis and hypersensitivity to oxidants. Currently no pharmacological treatm...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2011.12.002
更新日期:2012-01-20 00:00:00
abstract::The ATPase Cdc48 is required for membrane fusion and protein degradation. Recently it has been suggested that Cdc48 in a complex with Ufd1 and Npl4 acts as an ubiquitin-dependent chaperone. Here it is shown that recombinant Cdc48 alone can distinguish between the native and the non-native conformation of model substra...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)02777-1
更新日期:2002-06-05 00:00:00
abstract::Polyphosphates (polyP) are long chains of inorganic phosphates that can be attached to lysine residues of target proteins as a nonenzymatic post-translational modification. This modification, termed polyphosphorylation, may be particularly prevalent in bacterial and fungal species that synthesize large quantities of p...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1002/1873-3468.13588
更新日期:2020-01-01 00:00:00
abstract::Sequence comparison based on Hydrophobic Cluster Analysis procedures shows that the extracellular approximately 200 amino acids domains of cytokines receptors belonging to the Cytokine/Growth hormone/Prolactin receptor family and to the Interferon one are organized in two homologous subdomains. Further, comparison of ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80437-8
更新日期:1991-04-22 00:00:00
abstract::We have shown previously that the skeletal muscle ryanodine receptor mRNA of approximately 16,000 nucleotides codes 5,037 amino acid residues constituting the calcium release channel in skeletal muscle. In this study, RNA blot hybridization analysis shows that the brain contains an RNA species with an estimated size o...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)81547-d
更新日期:1993-05-10 00:00:00
abstract::The tomato saponin alpha-tomatine has been proposed to kill sensitive cells by binding to cell membranes followed by leakage of cell components. However, details of the modes of action of the compound on fungal cells are poorly understood. In the present study, mechanisms involved in alpha-tomatine-induced cell death ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2007.06.010
更新日期:2007-07-10 00:00:00
abstract::Using radiolabeled specific cDNA glutamine synthetase mRNA could be detected by in situ hybridization exclusively within those few perivenous hepatocytes which stained immunocytochemically for glutamine synthetase. This localization of glutamine synthetase mRNA was recently reported by Moorman et al. [(1988) J. Histoc...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)81037-8
更新日期:1988-12-05 00:00:00
abstract::We have cloned and characterized four Itpk genes from soybean. All four recombinant Itpk proteins showed canonical Ins(1,3,4)P3 5/6-kinase activity, but a kinetic analysis raised questions about its biological significance. Instead, we provide evidence that one alternative biological role for soybean Itpks is to inter...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2008.04.054
更新日期:2008-06-11 00:00:00
abstract::Characterization of lignified Zinnia elegans hypocotyls by both alkaline nitrobenzene oxidation and thioacidolysis reveals that coniferyl alcohol units are mainly found as part of 4-O-linked end groups and aryl-glycerol-beta-aryl ether (beta-O-4) structures. Z. elegans hypocotyls also contain a basic peroxidase (EC 1....
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)03339-2
更新日期:2002-10-09 00:00:00
abstract::Decades of neuroscience research have shed light on the hippocampus as a key structure for the formation of episodic memory. The hippocampus is divided into distinct subfields - CA1, CA2 and CA3. While accumulating evidence points to cellular and synaptic heterogeneity within each subfield, this heterogeneity has not ...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2014.06.004
更新日期:2014-08-01 00:00:00
abstract::C6 rat glioma cells persistently infected with subacute sclerosing panencephalitis virus (C6/SSPE) were treated with measles antiserum and purified anti-measles IgG. This stimulated phosphoinositide breakdown and an increase in inositol phosphates. In uninfected C6 cells, however, only fetal calf serum (FCS), but not ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)81330-4
更新日期:1989-04-24 00:00:00
abstract::U46619 is a potent platelet agonist, its binding to the thromboxane A2 receptor resulting in Gq-binding protein-mediated responses; nevertheless, it is unable to cause platelet aggregation, unless released ADP is present. In this study we demonstrate that Gi activation is the step U46619 lacks to cause platelet aggreg...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)01049-7
更新日期:1998-09-11 00:00:00
abstract::The kinetic constants of internalization of asialoorosomucoid were determined for normal and jejuno-ileal by-passed rat hepatocytes. In by-passed rats the maximum velocity of asialoorosomucoid internalization is decreased 3-fold, without any modification of apparant constant of internalization. Moreover, the rate cons...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(85)80783-3
更新日期:1985-04-22 00:00:00
abstract::The synthesis of gangliosides GM3 and GD3 is carried out by the successive addition of sialic acid residues on lactosylceramide (LacCer) by the Golgi located sialyltransferases Sial-T1 and Sial-T2, respectively. CHO-K1 cells lack Sial-T2 and only express GM3. Here we show that the activity of Sial-T1 was near 2.5-fold...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2012.05.041
更新日期:2012-07-30 00:00:00
abstract::All eukaryotic cells enclose their genome within a dedicated, membrane-bound organelle termed the nucleus, which functions to partition gene transcription from sites of protein translation in the cytoplasm. Despite a great deal of research effort, basic questions about chromosome structure and gene expression mechanis...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2008.04.029
更新日期:2008-06-18 00:00:00
abstract::Structures and regulations of vertebrate channels responsible for sustained calcium elevations after hormone stimulation are largely unknown. Therefore, the Drosophila photoreceptor channels, trp and trpl, which are assumed to be involved in calcium influx, serve as model system, trpl expressed in Sf9 cells showed spo...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)01455-a
更新日期:1995-01-30 00:00:00
abstract::Rho-associated kinase 2 (Rock2) is known to promote tumorigenesis in hepatocellular carcinoma (HCC). CCAAT/enhancer-binding protein delta (CEBPD) functions as a tumor suppressor. In this study, we found that the expression of Rock2 and CEBPD are inversely correlated. Knockdown of Rock2 increased CEBPD expression and i...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2015.03.004
更新日期:2015-04-13 00:00:00
abstract::The proteolysis of beta-amyloid (Abeta) requires neprylisin, an enzyme that has been shown as reduced in Alzheimer's disease (AD). We investigated whether a decrease in neprilysin levels contributes to the accumulation of amyloid deposits not only in AD but also in the normal aging. We analyzed neprilysin mRNA and pro...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.09.054
更新日期:2005-11-07 00:00:00
abstract::Two major embryonic myosin heavy chains are expressed in embryonic chick breast muscle until the first week after hatching. Of these, one is already detected in the 8-day-old embryo. The other appears on day 12. Two putative slow embryonic isoforms represent minor components transiently expressed between days 8 and 12...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)80488-5
更新日期:1988-10-10 00:00:00
abstract::A hybrid protein from Neisseria meningitidis, which contains both a peroxiredoxin and a glutaredoxin domain, has been isolated. The enzyme was active in the reduction of various peroxides and dehydroascorbate in the presence of reduced glutathione. These findings suggest that both the peroxiredoxin and glutaredoxin do...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(03)01156-6
更新日期:2003-11-06 00:00:00
abstract::The reversible folding/unfolding of a short peptide in solution is studied by molecular dynamics simulations. The effects of long-range electrostatic interactions are examined and found to be important both for the equilibrium between folded and unfolded states and the dynamics of the folding process. The neglect of l...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)01079-0
更新日期:1999-09-03 00:00:00
abstract::We have used DNase I footprinting to examine the effect of a novel naphthylquinoline dimer, designed as a triplex-specific bis-intercalator, on the stability of intermolecular DNA triplexes. We find that this compound efficiently promotes triplex formation between the 9-mer oligonucleotide 5'-TTTTTTCTT and its oligopu...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00284-7
更新日期:1999-03-26 00:00:00