Utilization of alanine for glucose formation in isolated rabbit kidney-cortex tubules.

abstract：:A PCR-based subtractive cloning procedure was used to identify genes expressed at higher levels in the pancreatic beta cell line betaTC1, as compared to the pancreatic alpha cell line alphaTC1. One of the clones isolated by this procedure corresponded to the regulatory subunit (RIalpha) of protein kinase A (PKA). Usin...

journal_title：FEBS letters

authors： Arava Y,Adamsky K,Belleli A,Shaltiel S,Walker MD

更新日期：1998-03-20 00:00:00

abstract：:Only recently have products of the enzymatic breakdown of the chlorophylls been characterized for the first time. All catabolites isolated until now from a chlorophyte and from angiosperms have in common the position at which the primary oxygenative ring cleavage occurs, yielding linear tetrapyrroles (19-formyl-1[21H,...

journal_title：FEBS letters

authors： Curty C,Engel N,Gossauer A

更新日期：1995-05-01 00:00:00

abstract：:In fibrous connective tissues, fibroblasts are organized into syncytia, cellular networks that enable matrix remodeling and that are interconnected by intercellular adherens junctions (AJs). The AJs of fibroblasts are mediated by N-cadherin, a broadly expressed classical cadherin that is critically involved in develop...

journal_title：FEBS letters

authors： El Sayegh TY,Kapus A,McCulloch CA

更新日期：2007-01-23 00:00:00

abstract：:NADP-malic enzyme (NADP-ME) is a widely distributed enzyme that catalyzes the oxidative decarboxylation of L-malate. Photosynthetic NADP-MEs are found in C4 bundle sheath chloroplasts and in the cytosol of CAM plants, while non-photosynthetic NADP-MEs are either plastidic or cytosolic in various plants. We propose a c...

journal_title：FEBS letters

authors： Drincovich MF,Casati P,Andreo CS

更新日期：2001-02-09 00:00:00

abstract：:Arginine kinase catalyzes the reversible transphosphorylation between adenosine diphosphate (ADP) and phosphoarginine, which is involved in temporal and spatial adenosine triphosphate (ATP) buffering. Here we demonstrate that the homologous overexpression of the Trypanosoma cruzi arginine kinase improves the ability o...

journal_title：FEBS letters

authors： Pereira CA,Alonso GD,Ivaldi S,Silber AM,Alves MJ,Torres HN,Flawiá MM

更新日期：2003-11-06 00:00:00

abstract：:Redox homeostasis is a prerequisite for survival of the pathogen Mycobacterium tuberculosis (Mtb) which employs the low molecular weight thiol mycothiol (MSH). The Mycobacterial NADPH-dependent mycothione reductase (MtMtr), composed of an NADPH-, FAD-, and a dimerization-domain connected by linkers, regulates the bala...

journal_title：FEBS letters

authors： Kumar A,Subramanian Manimekalai MS,Grüber G

更新日期：2018-02-01 00:00:00

abstract：:The hirudin-like factor 1 (HLF1) of Hirudo medicinalis belongs to a new class of leech-derived factors. In previous investigations, HLF1 did not exhibit anticoagulatory activities. Here, we describe the analysis of natural and synthetic variants of HLF1 and HLF-Hyb, a yet uncharacterized member of the HLF family. Modi...

journal_title：FEBS letters

authors： Müller C,Lukas P,Böhmert M,Hildebrandt JP

更新日期：2020-03-01 00:00:00

abstract：:The thermophilic cyanobacterial strain, PCC7518, originally identified as 'Mastigocladus laminosus HTF' does not show branchings or heterocysts. The absence of branchings supports the later assignment to the genus Chlorogloeopsis. The absence of heterocysts may be the result of a mutation because heterocysts were obse...

journal_title：FEBS letters

authors： Wilmotte A,Van der Auwera G,De Wachter R

更新日期：1993-02-08 00:00:00

abstract：:PPP2R2C encodes a gamma isoform of the subunit B55 subfamily, which is a regulatory subunit of Protein phosphatase type 2A (PP2A). Our study shows that PPP2R2C is downregulated in glioma cells and human brain cancer patient samples. Overexpression of PPP2R2C inhibited cancer cell proliferation both in vitro and in viv...

journal_title：FEBS letters

authors： Fan YL,Chen L,Wang J,Yao Q,Wan JQ

更新日期：2013-12-11 00:00:00

abstract：:Leonor Michaelis spent the years of 1922-1926 as Professor of Biochemistry of the Aichi Medical College (now Graduate School of Medicine, Nagoya University) in Nagoya, Japan. Michaelis succeeded in gathering many bright young biochemists from all over Japan into his laboratory, and made tremendous contributions to the...

journal_title：FEBS letters

authors： Nagatsu TT

更新日期：2013-09-02 00:00:00

abstract：:We have studied the endosome-lysosome transfer of internalized epidermal growth factor receptor (EGFR) complexes in a cell-free system from rat liver. Analytical subfractionation of a postmitochondrial supernatant fraction showed that a pulse of internalized [(125)I]EGF was largely associated with a light endosomal fr...

journal_title：FEBS letters

authors： Authier F,Chauvet G

更新日期：1999-11-12 00:00:00

abstract：:Transgenic Escherichia coli expressing pyrroloquinoline-quinone (PQQ) synthase gene from Deinococcus radiodurans showed superior survival during Rose Bengal induced oxidative stress. Such cells showed significantly low levels of protein carbonylation as compared to non-transgenic control. In vitro, PQQ reacted with re...

journal_title：FEBS letters

authors： Misra HS,Khairnar NP,Barik A,Indira Priyadarsini K,Mohan H,Apte SK

更新日期：2004-12-03 00:00:00

abstract：:It is known that chemokine-like factor superfamily 8 (CKLFSF8), a member of the CKLF superfamily, has four putative transmembrane regions and a MARVEL domain. Its structure is similar to TM4SF11 (plasmolipin) and widely distributed in normal tissue. However, its function is not yet known. We show here that CKLFSF8 is ...

journal_title：FEBS letters

authors： Jin C,Ding P,Wang Y,Ma D

更新日期：2005-11-21 00:00:00

abstract：:We developed a novel method to quantitatively measure GTP gamma S binding to specific G proteins in crude membranes using G-protein antibodies. The basic strategy was that the materials were initially incubated with [35S]GTP gamma S at 37 degrees C. After 4 degrees C incubation in the wells of an ELISA plate precoated...

journal_title：FEBS letters

authors： Okamoto T,Ikezu T,Murayama Y,Ogata E,Nishimoto I

更新日期：1992-06-29 00:00:00

abstract：:Coronin proteins are widely expressed among eukaryotic organisms. Most coronins consist of a WD-repeat domain followed by a C-terminal coiled coil. Dictyostelium discoideum expresses a single short coronin coronin A, which has been implicated in both actin modulation and multicellular differentiation. Whether coronin ...

journal_title：FEBS letters

authors： Fiedler T,Fabrice TN,Studer V,Vinet A,Faltova L,Kammerer RA,Steinmetz MO,Sharpe T,Pieters J

更新日期：2020-04-16 00:00:00

abstract：:Photosystem I (PSI) complex of Anabaena variabilis ATCC 29413 consists of at least 11 subunits, 9 of which are resolved by high resolution gel electrophoresis. N-terminal amino acid sequences of the four subunits with molecular masses of 6.8, 5.2, 4.8 and 3.5 kDa were determined. Based on the sequence homology, the 3....

journal_title：FEBS letters

authors： Ikeuchi M,Nyhus KJ,Inoue Y,Pakrasi HB

更新日期：1991-08-05 00:00:00

abstract：:We have examined the properties of intramolecular G-quadruplexes in which the G3 tracts are separated by single base loops. The most stable complex contained 1',2'-dideoxyribose in all three loops, while loops containing T and C were slightly less stable (by about 2 degrees C). Quadruplexes containing loops with singl...

journal_title：FEBS letters

authors： Rachwal PA,Brown T,Fox KR

更新日期：2007-04-17 00:00:00

abstract：:Adrenomedullin, a potently hypotensive peptide isolated from human pheochromocytoma, is known to elicit a rise in cAMP levels within mammalian endothelial and smooth muscle cells. Until now, however, little has been known about the adrenomedullin receptor. Recently, a group called receptor activity-modifying proteins ...

journal_title：FEBS letters

authors： Kamitani S,Asakawa M,Shimekake Y,Kuwasako K,Nakahara K,Sakata T

更新日期：1999-04-01 00:00:00

abstract：:The matrix protein M of Borna disease virus (BDV) is a constituent of the viral envelope covering the inner leaflet of the lipid bilayer. BDV-M was expressed as recombinant protein in Escherichia coli, purified to homogeneity and structurally analyzed. Recombinant M (i) forms non-covalently bound multimers with a Stok...

journal_title：FEBS letters

authors： Kraus I,Bogner E,Lilie H,Eickmann M,Garten W

更新日期：2005-05-09 00:00:00

abstract：:Side chain-hydroxylated derivatives of cholesterol (OH sterol) inhibiting lymphoblastic transformation bind with high affinity and specificity to a hydroxysterol binding protein (OHSBP) in the cytosol of human lymphocytes. These binding properties of OHSBP suggested some analogies with that of steroid hormone receptor...

journal_title：FEBS letters

authors： Defay R,Astruc M,Beseme F,Descomps B,Crastes de Paulet A

更新日期：1984-08-06 00:00:00

abstract：:A 33-kDa protein component of the oxygen-evolving complex in photosystem II is essential for photosynthesis, and it has been believed that mutants with deletion of this 33-kDa protein are not found in higher plants. We report here the first isolation of an Arabidopsis thaliana mutant with a defect in one of the genes ...

journal_title：FEBS letters

authors： Murakami R,Ifuku K,Takabayashi A,Shikanai T,Endo T,Sato F

更新日期：2002-07-17 00:00:00

abstract：:2-( Dimethylaminostyryl )-l-methyl pyridinium, a fluorescent indicator of mitochondrial membrane potential, is accumulated up to 400-fold by intact, isolated brown adipocytes from cold-adapted guinea pigs. The indicator can be optically localized within the in situ mitochondria. Ionophores which would be predicted to ...

journal_title：FEBS letters

authors： Rafael J,Nicholls DG

更新日期：1984-05-07 00:00:00

abstract：:Protein kinase D (PKD)/protein kinase Cmicro (PKCmicro, a serine/threonine protein kinase with distinct structural and enzymological properties, is rapidly activated in intact cells via PKC. The amino-terminal region of PKD contains a cysteine-rich domain (CRD) that directly binds phorbol esters with a high affinity. ...

journal_title：FEBS letters

authors： Matthews S,Iglesias T,Cantrell D,Rozengurt E

更新日期：1999-09-03 00:00:00

abstract：:The inhibition of electron transport by unnatural (-)-antimycin A3 was examined with rat liver mitochondria and compared with that of natural (+)-antimycin A3. (-)-Antimycin A3 inhibited respiration about 1/100th as strongly as natural (+)-antimycin A3. (-)-Antimycin A3 binding to the cytochrome bc1 complex did not se...

journal_title：FEBS letters

authors： Miyoshi H,Kondo H,Oritani T,Saitoh I,Iwamura H

更新日期：1991-11-04 00:00:00

abstract：:Zinc (Zn) deficiency is often associated with low plasma vitamin A (retinol) concentrations. It has been suggested that the reduction in plasma retinol is secondary to reduced liver retinol binding protein (RBP) synthesis. In the present study, RBP expression was determined in HepG2 cells cultured in either Zn adequat...

journal_title：FEBS letters

authors： Satre MA,Jessen KA,Clegg MS,Keen CL

更新日期：2001-03-02 00:00:00

abstract：:Selective protein degradation is an energy-dependent process performed by high-molecular-weight proteases. The activity of proteolytic components of these enzymes is coupled to the ATPase activity of their regulatory subunits or domains. Here, we obtained the proteolytic domain of Escherichia coli protease Lon by clon...

journal_title：FEBS letters

authors： Rasulova FS,Dergousova NI,Starkova NN,Melnikov EE,Rumsh LD,Ginodman LM,Rotanova TV

更新日期：1998-08-07 00:00:00

abstract：:HMr alpha 2CPI was found to be an inhibitor of human liver cathepsin H by the measurement of the dissociation constant (Ki), the association rate constant (k1) and the dissociation rate constant (k-1) between the enzyme and the inhibitor. These data suggest that this protein-proteinase inhibitor can play a physiologic...

journal_title：FEBS letters

authors： Pagano M,Engler R

更新日期：1984-01-23 00:00:00

abstract：:In a wild-type strain of Saccharomyces cerevisiae, cadmium induces the activities of both gamma-glutamyl transferase (gamma-GT) and glutathione transferase 2 (Gtt2). However, Gtt2 activity did not increase under gamma-GT or Ycf1 deficiencies, suggesting that the accumulation of glutathione-cadmium in the cytosol inhib...

journal_title：FEBS letters

authors： Adamis PD,Mannarino SC,Eleutherio EC

更新日期：2009-05-06 00:00:00

abstract：:In the bovine ovary there is a delay of 4-6 days between the observed maximum of oxytocin mRNA and the peak in the luteal levels of oxytocin nonapeptide. This implies a maturation process involving components of the post-translational processing pathway. In situ hybridization shows the oxytocin gene to be transcribed ...

journal_title：FEBS letters

authors： Fehr S,Ivell R,Koll R,Schams D,Fields M,Richter D

更新日期：1987-01-01 00:00:00

abstract：:Recently mutations in the LGI1 (leucine-rich, glioma-inactivated 1) gene have been found in human temporal lobe epilepsy. We have now identified three formerly unknown LGI-like genes. Hydropathy plots and pattern analysis showed that LGI genes encode proteins with large extra- and intracellular domains connected by a ...

journal_title：FEBS letters

authors： Gu W,Wevers A,Schröder H,Grzeschik KH,Derst C,Brodtkorb E,de Vos R,Steinlein OK

更新日期：2002-05-22 00:00:00