Arginine kinase overexpression improves Trypanosoma cruzi survival capability.

abstract：:Monoamine oxidase B (MAO B) is an outer mitochondrial membrane protein that oxidizes arylalkylamine neurotransmitters and has been a valuable drug target for many neurological disorders. The 1.7 angstrom resolution structure of human MAO B shows the enzyme is dimeric with a C-terminal transmembrane helix protruding fr...

journal_title：FEBS letters

authors： Binda C,Hubálek F,Li M,Edmondson DE,Mattevi A

更新日期：2004-04-30 00:00:00

abstract：:The possible participation of calmodulin in the activation of store-operated Ca2+ entry (SOC) in single rat hepatocytes was investigated microspectrofluorimetrically. SOC was triggered after discharging intracellular Ca2+ stores using the endoplasmic reticulum Ca2+-ATPase inhibitor thapsigargin in the absence of exter...

journal_title：FEBS letters

authors： Cao Y,Chatton JY

更新日期：1998-03-06 00:00:00

abstract：:The rotary motion in response to ATP hydrolysis of the ring of c subunits of the membrane portion, F(o), of ATP synthase, F(o)F(1), is still under contention. It was studied with EF(o)EF(1) (Escherichia coli) using microvideography with a fluorescent actin filament. To overcome the limited specificity of actin attachm...

journal_title：FEBS letters

authors： Pänke O,Gumbiowski K,Junge W,Engelbrecht S

更新日期：2000-04-21 00:00:00

abstract：:Glucocorticoid-induced TNFR-related protein (GITR) is constitutively expressed in T regulatory (Treg) cells and regulates their suppressive function. We identified two methylated CpG islands in the Gitr locus. Using a ChIP assay, we demonstrate that both DNMT1 and methyl-CpG-binding domain Protein 4 (MBD4) bind to the...

journal_title：FEBS letters

authors： Wang S,Li Y,Zhu F,Lin F,Luo X,Zhao B,Zhang P,Li D,Gao Y,Liang R,Liu L,Tsun A,Yuan X,Wu K,Li B

更新日期：2017-07-01 00:00:00

abstract：:Endothelin (ET-1) has been shown to exert vasoconstrictor activity in vivo and mobilize Ca2+ in vascular smooth muscle cells in culture. In this paper we show that the human skin fibroblast exhibits specific receptors to ET-1 and that activation of these receptors results in increased intracellular Ca2+ (Ca2+i) and ac...

journal_title：FEBS letters

authors： Gardner JP,Maher E,Aviv A

更新日期：1989-10-09 00:00:00

abstract：:Biochemical, immunohistochemical and molecular biological methods were used to detect fetal myosin heavy chain (MyHC) in the human masseter of elderly and young subjects. Samples from the elderly subjects contained larger amounts of fetal MyHC than those of young adults. Only a very small amount of embryonic MyHC coul...

journal_title：FEBS letters

authors： Monemi M,Eriksson PO,Dubail I,Butler-Browne GS,Thornell LE

更新日期：1996-05-13 00:00:00

abstract：:hsp25 is a small, growth-related, mammalian stress protein which is highly accumulated in the stationary phase of Ehrlich ascites tumor in vivo. Ehrlich ascites cells cultivated in vitro under conditions of continuous exponential growth express hsp25 only at a low level. These cells were stably transfected with an euk...

journal_title：FEBS letters

authors： Knauf U,Bielka H,Gaestel M

更新日期：1992-09-14 00:00:00

abstract：:It had been shown previously by heterologous expression in Schizosaccharomyces pombe, that the two monosaccharide/H+ symporters HUP1 and HUP2 of Chlorella kessleri differ significantly concerning their substrate specificity: HUP1 transports predominantly D-glucose while HUP2 prefers D-galactose. Several chimeric trans...

journal_title：FEBS letters

authors： Will A,Tanner W

更新日期：1996-02-26 00:00:00

abstract：:The inositol (1,4,5)-trisphosphate receptor (InsP3R) is an intracellular calcium release channel that plays a crucial role in cell signaling. In Drosophila melanogaster, a single InsP3R gene (itpr) encodes a protein (DmInsP3R) that is approximately 60% conserved with mammalian InsP3Rs. The functional properties of wil...

journal_title：FEBS letters

authors： Srikanth S,Wang Z,Hasan G,Bezprozvanny I

更新日期：2004-09-24 00:00:00

abstract：:The cardiac and skeletal muscle isoforms of calsequestrin (CS), the low affinity, high capacity Ca2+ binding protein localized in the lumen of sarcoplasmic reticulum, are the products of two different genes (Fliegel, L., Leberer, E., Green, N.M. and MacLennan, D.H. (1982) FEBS Lett. 242, 297-300), and can be both puri...

journal_title：FEBS letters

authors： Biral D,Volpe P,Damiani E,Margreth A

更新日期：1992-03-09 00:00:00

abstract：:The NK-lysin derived peptide NK-2 is a potent antibacterial, but non-toxic to a human keratinocyte cell line and of low hemolytic activity. Its target selectivity is based upon a strong binding preference to membranes containing anionic phospholipids, which are normally not found on the surface of human cells. Here, w...

journal_title：FEBS letters

authors： Schröder-Borm H,Bakalova R,Andrä J

更新日期：2005-11-07 00:00:00

abstract：:Extracellular regulated kinases (ERKs) mediate the inhibitory effect of tumor necrosis factor alpha (TNF-alpha) on uncoupling protein-1 (UCP-1), but not on lipid accumulation. TNF-alpha-induced ERK-dependent peroxisome proliferator activator receptor gamma (PPAR gamma) phosphorylation could be responsible for UCP-1 do...

journal_title：FEBS letters

authors： Porras A,Valladares A,Alvarez AM,Roncero C,Benito M

更新日期：2002-06-05 00:00:00

abstract：:Early in the course of pregnancy, at the preimplantation stage, the pig embryo is likely to exert a paracrine effect on the tissue intended to receive it, via the secretion of interferons. Our observations show that trophoblastic interferons induce an increase of some mRNAs in the epithelial cells of the gilt endometr...

journal_title：FEBS letters

authors： Chwetzoff S,d'Andrea S

更新日期：1997-03-24 00:00:00

abstract：:Plasma citrate levels were found to be elevated in non-alcoholic fatty liver disease (NAFLD) patients. Cellular experiments indicated that increased citrate levels might originate from an excess of fatty acids. The impact of elevated citrate levels on oxidative stress was examined. It was found that citrate stimulated...

journal_title：FEBS letters

authors： van de Wier B,Balk JM,Haenen GR,Giamouridis D,Bakker JA,Bast BC,den Hartog GJ,Koek GH,Bast A

更新日期：2013-08-02 00:00:00

abstract：:Disruption of the gene pgil of Saccharomyces cerevisiae, which codes for phosphoglucose isomerase, results in a dramatic increase in the amount of intracellular glycogen in early exponential cultures. The level of glucose 6-phosphate was much higher in mutant than in wild-type cells. Phosphorylase a activity and the s...

journal_title：FEBS letters

authors： Corominas J,Clotet J,Fernández-Bañares I,Boles E,Zimmermann FK,Guinovart JJ,Ariño J

更新日期：1992-09-28 00:00:00

abstract：:The maturation of Borna disease virus (BDV) glycoprotein GP was studied in regard to intracellular compartmentalization, compartmentalization signal-domains, proteolytic processing, and packaging into virus particles. Our data show that BDV-GP is (i) predominantly located in the endoplasmic reticulum (ER), (ii) partia...

journal_title：FEBS letters

authors： Eickmann M,Kiermayer S,Kraus I,Gössl M,Richt JA,Garten W

更新日期：2005-08-29 00:00:00

abstract：:The primary structure of 20 beta-hydroxysteroid dehydrogenase from Streptomyces hydrogenans was determined after FPLC purification of a commercial preparation. Peptides obtained from different proteolytic cleavages were purified by reverse phase HPLC. The 255-residue structure deduced was found to be distantly homolog...

journal_title：FEBS letters

authors： Marekov L,Krook M,Jörnvall H

更新日期：1990-06-18 00:00:00

abstract：:We investigated 38 cases of B-cell chronic lymphocytic leukemia (B-CLL) for the presence of non-productive rearrangements in the S(mu) regions and defined for the first time the molecular nature of these rearrangements. Southern blot analysis revealed S(mu) region rearrangements in 13 cases (34%) and polymerase chain ...

journal_title：FEBS letters

authors： Nardini E,Rizzi S,Capello D,Vitolo U,Gaidano G,Menard S,Balsari A

更新日期：2002-05-08 00:00:00

abstract：:The genetic modification of lysozyme was attempted to improve the bactericidal activity against Gram-negative bacteria E. coli. The different lengths of hydrophobic peptides were attached to the C-terminus of the hen egg white lysozyme to investigate the most effective length of the hydrophobic peptides for killing ba...

journal_title：FEBS letters

authors： Arima H,Ibrahim HR,Kinoshita T,Kato A

更新日期：1997-09-22 00:00:00

abstract：:Many receptors coupled to inhibitory Go/Gi-type G proteins often also produce stimulatory signals like Ca2+ mobilisation. When expressed in CHO cells the alpha2-adrenoceptor subtypes alpha2A, alpha2B and alpkha2C mobilised Ca2+. These responses were strongly reduced by the P2Y-purinoceptor antagonist suramin. A large ...

journal_title：FEBS letters

authors： Akerman KE,Näsman J,Lund PE,Shariatmadari R,Kukkonen JP

更新日期：1998-07-03 00:00:00

abstract：:The cDNA coding for mature human alpha 1-proteinase inhibitor (alpha 1-PI) has been inserted into a variety of yeast expression vectors. Yeast cells transformed with these plasmids were then assayed for the production of mature, unglycosylated alpha 1-PI. The production level is optimal when the recombinant plasmid ca...

journal_title：FEBS letters

authors： Hoylaerts M,Weyens A,Bollen A,Harford N,Cabezón T

更新日期：1986-08-11 00:00:00

abstract：:The heat shock factor (HSF1) is the central regulator of the heat stress (hs) response and is required for stimulating the transcription of the hs genes and consequently the expression of heat shock proteins. To promote the polymerase II-dependent transcription of the hs genes, HSF has to communicate with the basal tr...

journal_title：FEBS letters

authors： Reindl A,Schöffl F

更新日期：1998-10-09 00:00:00

abstract：:The majority of cases of early-onset familial Alzheimer disease are caused by mutations in the recently identified presenilin 1 (PS1) gene, located on chromosome 14. PS1, a 467 amino acid protein, is predicted to be an integral membrane protein containing seven putative transmembrane domains and a large hydrophilic lo...

journal_title：FEBS letters

authors： Mercken M,Takahashi H,Honda T,Sato K,Murayama M,Nakazato Y,Noguchi K,Imahori K,Takashima A

更新日期：1996-07-08 00:00:00

abstract：:The KDEL receptor is a seven-transmembrane-domain protein that was first described about 20 years ago. Its well-known function is to retrotransport chaperones from the Golgi complex to the endoplasmic reticulum. Recent studies, however, have suggested that the KDEL receptor has additional functions. Indeed, we have de...

journal_title：FEBS letters

authors： Capitani M,Sallese M

更新日期：2009-12-03 00:00:00

abstract：:Prokaryotes, plants and animals control ammonium fluxes by the regulated expression of ammonium transporters (AMTs) and/or the related Rhesus (Rh) proteins. Plant AMTs were previously reported to mediate electrogenic transport. Functional analysis of AtAMT2 from Arabidopsis in yeast and oocytes suggests that NH(4)(+) ...

journal_title：FEBS letters

authors： Neuhäuser B,Dynowski M,Ludewig U

更新日期：2009-09-03 00:00:00

abstract：:We investigated expression levels of Na(+),K(+)-ATPase alpha-isoforms and their ATPase activities in human colorectal cancer tissue and the accompanying normal mucosa. A decrease in expression of the alpha1-isoform protein was observed in all sampled cancer tissues compared with the normal mucosae. The level of ouabai...

journal_title：FEBS letters

authors： Sakai H,Suzuki T,Maeda M,Takahashi Y,Horikawa N,Minamimura T,Tsukada K,Takeguchi N

更新日期：2004-04-09 00:00:00

abstract：:Peroxynitrite (ONOO-) has recently been implicated in connective tissue destruction in vivo. We have studied the effect of ONOO- on the activity of tissue inhibitor of metalloproteinase-1 (TIMP-1) in vitro. The inactivation of TIMP-1 by ONOO- was dose dependent with 50 microM ONOO- reducing the inhibitory activity of ...

journal_title：FEBS letters

authors： Frears ER,Zhang Z,Blake DR,O'Connell JP,Winyard PG

更新日期：1996-02-26 00:00:00

abstract：:γ-Glutamyl kinase (GK; the PRO1 gene product) is a key enzyme in the Saccharomyces cerevisiae proline biosynthesis pathway. Δpro1 cells are more sensitive to various stresses than wild-type cells, suggesting that GK has an alternative function independent of proline biosynthesis. We show that PRO1 genetically interact...

journal_title：FEBS letters

authors： Tatehashi Y,Watanabe D,Takagi H

更新日期：2016-09-01 00:00:00

abstract：:Prominin-1 (CD133) is a cholesterol-interacting pentaspan membrane protein concentrated in plasma membrane protrusions. In epithelial cells, notably neuroepithelial stem cells, prominin-1 is found in microvilli, the primary cilium and the midbody. These three types of apical membrane protrusions are subject to remodel...

journal_title：FEBS letters

authors： Corbeil D,Marzesco AM,Wilsch-Bräuninger M,Huttner WB

更新日期：2010-05-03 00:00:00

abstract：:A new approach is suggested for delineating the structural and functional amino acid residues in proteins with known three-dimensional structure, basing on the involvement of residues in intramolecular hydrophobic and hydrophilic interactions and additional information about the conservativity of the residues. The app...

journal_title：FEBS letters

authors： Golovanov AP,Efremov RG,Jaravine VA,Vergoten G,Arseniev AS

更新日期：1995-11-13 00:00:00