Abstract:
:The genetic modification of lysozyme was attempted to improve the bactericidal activity against Gram-negative bacteria E. coli. The different lengths of hydrophobic peptides were attached to the C-terminus of the hen egg white lysozyme to investigate the most effective length of the hydrophobic peptides for killing bacteria. The oligonucleotides encoding Phe-Val-Pro (H3), Phe-Phe-Val-Ala-Pro (H5) and Phe-Phe-Val-Ala-Ile-Ile-Pro (H7) were fused to the C-terminus Leu 129 of lysozyme cDNA. The reconstructed cDNAs were inserted into the yeast expression vector. The hydrophobic peptide-fused lysozymes were secreted in the yeast carrying the reconstructed cDNA. Although the hydrophobic peptide-fused lysozymes retained 75 80% lytic activity of the wild-type protein, the bactericidal action to E. coli was greatly increased with the length of hydrophobic peptides. These results suggest that the hydrophobic peptides play an important role in killing Gram-negative bacteria. To elucidate the role of catalytic domain in bactericidal action of the hydrophobic fusion lysozyme (H5-Lz), the mutant hydrophobic lysozyme (H5/E35A-Lz) whose glutamic acid was substituted with alanine at the position 35 was constructed to diminish the catalytic activity. The mutant hydrophobic lysozyme (H5/E35A-Lz) was greatly lost the bactericidal action to E. coli, suggesting that not only the length of hydrophobic peptide fused to C-terminus but also the catalytic domain is important for the bactericidal action of the hydrophobic peptide-fused lysozyme.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Arima H,Ibrahim HR,Kinoshita T,Kato Adoi
10.1016/s0014-5793(97)01071-5subject
Has Abstractpub_date
1997-09-22 00:00:00pages
114-8issue
1eissn
0014-5793issn
1873-3468pii
S0014-5793(97)01071-5journal_volume
415pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::hsp25 is a small, growth-related, mammalian stress protein which is highly accumulated in the stationary phase of Ehrlich ascites tumor in vivo. Ehrlich ascites cells cultivated in vitro under conditions of continuous exponential growth express hsp25 only at a low level. These cells were stably transfected with an euk...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80793-g
更新日期:1992-09-14 00:00:00
abstract::A selective PKC inhibitor, UCN-01, was shown to exhibit anti-tumor activity in vitro and in vivo. We investigated UCN-01 with respect to isozyme-specific PKC inhibition using purified recombinant or rabbit brain PKC isozymes, cPKC alpha, beta and gamma, nPKC delta, epsilon and eta, and a PKC zeta. Of the PKC isozymes ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)00042-8
更新日期:1995-02-13 00:00:00
abstract::Transcription of Drosophila U1 or U6 snRNAs by RNA polymerases II and III respectively requires a unique approximately 21 base-pair promoter element termed the proximal sequence element A (PSEA) recognized by the snRNA activating protein complex (DmSNAPc). A five-nucleotide substitution that changed the U1 PSEA to a U...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2008.06.003
更新日期:2008-07-09 00:00:00
abstract::The structure and organization of five genes responsible for the synthesis of six isoforms of short-chain alpha-neurotoxins in Pseudonaja textilis venom are reported in this paper. This also forms the first report which describes the synthesis of two neurotoxin mRNA variants from one of these genes (Pt-sntx1) as a res...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)01549-0
更新日期:2000-05-19 00:00:00
abstract::Caloric restriction (CR) is known to promote longevity in various species. Sirtuin-mediated deacetylation has been shown to be related to the promotion of longevity in some species. Here, we show that CR of rats led to an increase in the level of Werner syndrome protein (WRN), a recognized DNA repair protein. In addit...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2008.06.031
更新日期:2008-07-23 00:00:00
abstract::Tyrosine O-sulfation is one of the post-translational modification processes that occur to membrane proteins and secreted proteins in eukaryotes. Tyrosylprotein sulfotransferase (TPST) is responsible for this modification, and in this report, we describe the expression pattern and the biological role of TPST-A in the ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2004.11.044
更新日期:2005-01-03 00:00:00
abstract::Colneleic acid (9-[1'(E),3'(Z)-nonadienyloxy]-8(E)-nonenoic acid) is produced from linoleic acid by the sequential action of 9-lipoxygenase and divinyl ether synthase. We demonstrate that a small fraction of the colneleic acid in potato tubers is esterified in phospholipids. This colneleic acid was released by chemica...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(03)00171-6
更新日期:2003-03-13 00:00:00
abstract::We have identified an Arabidopsis thaliana cDNA, designated as AtTBP1, encoding a protein with a predicted size of 70.6 kDa that specifically binds to the plant telomeric repeat sequence TTTAGGG. AtTBP1 is present as a single-copy gene in Arabidopsis genome and is expressed ubiquitously in various organs. AtTBP1 has a...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)02685-0
更新日期:2001-08-10 00:00:00
abstract::The geometries are reported for interacting arginine-carboxyl pairs obtained from 37 high resolution protein structures solved to a resolution of 2.0 A or better. The closest interatomic distance between the guanidinium and carboxyl is less than 4.2 A for 74 arginine and carboxyl groups, with the majority of these lyi...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(87)80441-6
更新日期:1987-11-16 00:00:00
abstract::It is clear that G1-S phase control is exerted after the mouse embryo implants into the uterus 4.5 days after fertilization (E4.5); null mutants of genes that control cell cycle commitment such as max, rb (retinoblastoma), and dp1 are embryonic lethal after implantation with proliferation phenotypes. But, a number of ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2004.10.109
更新日期:2005-01-17 00:00:00
abstract::A cDNA clone (pUP-23) corresponding to a member of a protein family that includes inhibitors of trypsin and of heterologous alpha-amylases has been selected from a library derived from developing barley endosperm and its sequence has been determined. A stretch of 95 nucleotides that included the signal peptide and the...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)80562-3
更新日期:1988-10-24 00:00:00
abstract::Plant receptor-like kinases (RLKs) share their evolutionary origin with animal interleukin-1 receptor-associated kinase (IRAK)/Pelle family of soluble kinases and are distinguished by having tyrosine as 'gatekeeper'. This position is adjacent to the hinge region and is hidden in a hydrophobic pocket of the catalytic c...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2013.07.050
更新日期:2013-09-17 00:00:00
abstract::Glycogen storage disease type Ia (GSD-Ia) patients manifest a pro-atherogenic lipid profile but are not at elevated risk for developing atherosclerosis. Serum phospholipid, which correlates positively with the scavenger receptor class B type I (SR-BI)-mediated cholesterol efflux, and apolipoprotein A-IV and E, accepto...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.07.042
更新日期:2005-08-29 00:00:00
abstract::A major house dust mite allergen Der f 1 belongs to the papain-like cysteine protease family. This study investigated whether Der f 1 can cleave the latency-associated peptide (LAP) of transforming growth factor (TGF)-beta via its proteolytic activity and activate latent TGF-beta. We found that Der f 1 can cleave LAP ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2009.05.030
更新日期:2009-06-18 00:00:00
abstract::We investigated expression levels of Na(+),K(+)-ATPase alpha-isoforms and their ATPase activities in human colorectal cancer tissue and the accompanying normal mucosa. A decrease in expression of the alpha1-isoform protein was observed in all sampled cancer tissues compared with the normal mucosae. The level of ouabai...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/S0014-5793(04)00292-3
更新日期:2004-04-09 00:00:00
abstract::Lipopolysaccharide, the invariant structural component of Gram-negative bacteria, when present in minute amounts in the circulation in humans elicits 'endotoxic shock' syndrome, which is fatal in 60% of the cases. Polymyxin B (PMB), a cyclic cationic peptide, neutralizes the endotoxin, but also induces many harmful si...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00150-7
更新日期:1999-02-26 00:00:00
abstract::Renin, which catalyzes the initial proteolytic cleavage reaction in the production of angiotensins, is first synthesized as a zymogen, prorenin, and requires the proteolytic removal of an amino-terminal prosegment for activation in vivo. The lysosomal hydrolase cathepsin B has been proposed as a prorenin processing en...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)01672-x
更新日期:1999-01-22 00:00:00
abstract::The malaria parasite harbours an indispensable plastid known as the 'apicoplast'. The apicoplast's exact role remains uncertain, but it houses components involved in fatty acid, isoprenoid and haem biosyntheses. These pathways offer opportunities to develop anti-malarials. In the absence of photosynthesis, how apicopl...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2009.11.097
更新日期:2010-02-05 00:00:00
abstract::In Alzheimer's disease, beta-amyloid peptides (betaA(1-40) and betaA(1-42)) are deposited on the brain cell surfaces as neurotoxic plaques. Some reports indicate that small heat shock proteins, Hsp27 and alphaB-crystallin, colocalize in the plaques, but their functions are not known. Interaction between betaA and alph...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)02136-0
更新日期:2000-11-03 00:00:00
abstract::The NAD+-requiring enzymes of glycoside hydrolase family 4 (GHF4) contain a region with a conserved Gly-XXX-Gly-Ser (GXGS) motif near their N-termini that is reminiscent of the fingerprint region of the Rossmann fold, a conserved structural motif of classical nicotinamide nucleotide-binding proteins. The function of t...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)02641-8
更新日期:2002-04-24 00:00:00
abstract::Damage to the central nervous system triggers rapid activation and specific migration of glial cells towards the lesion site. There, glial cells contribute heavily to secondary neuronal changes that take place after lesion. In an attempt to identify the molecular cues of glial activation following brain trauma we perf...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/S0014-5793(04)00244-3
更新日期:2004-04-09 00:00:00
abstract::The effects of progesterone, dehydroepiandrosterone sulfate and 17beta-estradiol on the synthesis and degradation of hyaluronate were investigated using human uterine cervix fibroblasts. The cells were incubated with [3H]glucosamine in the presence of the hormones and then [3H]hyaluronate was isolated from the medium....
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(96)01538-4
更新日期:1997-02-03 00:00:00
abstract::A stable complex of the chaperonins, cpn60 and cpn10 (Escherichia coli GroEL and GroES homologues), from the extremely thermophilic bacterium Thermus thermophilus has been isolated and crystallized. The crystals have dimensions up to 30 x 200 x 200 microns. Ultra-thin sections of the crystals estimated by electron mic...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)81357-r
更新日期:1992-10-12 00:00:00
abstract::The effect of dexamethasone on mRNA and protein synthesis of lipocortins (LCT) 1, 2 and 5 has been investigated in U-937 cells. A constitutive expression of both mRNAs and proteins was detected in undifferentiated U-937 cells. This constitutive level was increased time- and dose-dependently by incubation with phorbol ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)81293-h
更新日期:1991-10-21 00:00:00
abstract::A number of cDNA clones in summary encoding 700 amino acid residues from the N-end of rat liver elongation factor 2 (EF-2) and including 49 nucleotides of the 5'-untranslated mRNA region have been obtained. EF-2 cDNA clones were isolated from gradually constructed small (1000-5000 clones) specific cDNA libraries using...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)80447-8
更新日期:1989-05-08 00:00:00
abstract::An abundant matrix protein was purified under native conditions from adult bovine tendon and identified as cartilage oligomeric matrix protein (COMP) by immunochemical crossreaction, amino acid sequence identity of tryptic peptides derived from both N- and C-terminal regions, and structure revealed by electron microsc...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)01134-6
更新日期:1994-11-07 00:00:00
abstract::The amyloid precursor protein (APP) is proteolytically processed by β- and γ-secretases to release amyloid-β peptide (Aβ), the main component found in senile plaques of Alzheimer's disease (AD) patient brains. Alternatively, APP can be cleaved within the Aβ sequence by α-secretase, thus precluding the generation of Aβ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2011.05.048
更新日期:2011-07-21 00:00:00
abstract::The influence of islet-activating protein (IAP), a Bordetella pertussis toxin, was studied on adenylate cyclase and GTPase activities in rat adipocyte membranes. Pretreatment of rats or intact rat adipocytes with IAP did not affect adenylate cyclase inhibition by the stable GTP analog, GTP gamma S, whereas inhibition ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)80254-3
更新日期:1983-05-30 00:00:00
abstract::Cytochrome oxidase exhibits phosphorescence from tryptophan in aqueous solution in the absence of oxygen. The lifetime for the resting reduced enzyme suspended in Tween-20 is around 30 ms at pH 8. The lifetime is longest between pH 7 and 8 and decreases with lowering of pH. Oxygen quenches the phosphorescence with a S...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80566-l
更新日期:1991-05-20 00:00:00
abstract::For almost 30 years, Wnt proteins have been known as key regulators of many developmental decisions, including the formation of the embryonic axes, patterning of the CNS, limb bud outgrowth and segment polarity. However, live cell imaging of active Wnt proteins was rarely reported. Here, we have generated a Wnt2b-EGFP...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2012.04.035
更新日期:2012-06-04 00:00:00