Abstract:
:Transcription of Drosophila U1 or U6 snRNAs by RNA polymerases II and III respectively requires a unique approximately 21 base-pair promoter element termed the proximal sequence element A (PSEA) recognized by the snRNA activating protein complex (DmSNAPc). A five-nucleotide substitution that changed the U1 PSEA to a U6 PSEA inactivated the U1 promoter. Chromatin immunoprecipitation assays indicated this substitution did not affect DmSNAPc DNA binding but instead interfered with SNAPc recruitment of TBP to the TATA-less U1 promoter. These findings support a model wherein sequence differences between the U1 and U6 PSEAs induce distinct DmSNAPc conformational states involved in RNA polymerase selectivity.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Barakat NH,Stumph WEdoi
10.1016/j.febslet.2008.06.003subject
Has Abstractpub_date
2008-07-09 00:00:00pages
2413-6issue
16eissn
0014-5793issn
1873-3468pii
S0014-5793(08)00488-2journal_volume
582pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::Dictyostelium discoideum Ax2 produces both L-erythro-tetrahydrobiopterin (BH4) and its stereoisomer D-threo-BH4 (DH4). The putative cofactor function of them for phenylalanine hydroxylase (PAH) was investigated through genetic manipulation and quantitative determination of pteridines. In addition to establishing that ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2011.08.026
更新日期:2011-10-03 00:00:00
abstract::Mycobacterium avium subsp. hominissuis (MAH) is an environmental bacterium causing opportunistic infections. The objective of this study was to identify flexible genome regions in MAH isolated from different sources. By comparing five complete and draft MAH genomes we identified a genomic island conferring additional ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2014.08.037
更新日期:2014-11-03 00:00:00
abstract::Adenosine can be metabolized by chicken erythrocytes as a carbon source maintaining ATP levels. In addition, it stimulates glucose uptake and lactate production. Adenosine effects do not involve binding to membrane receptors. They are dependent on the provision of a carbon source to glycolysis and correlate with the i...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)81635-7
更新日期:1989-11-20 00:00:00
abstract::We analysed the biochemical properties of insulin receptors of a Type A insulin resistant patient with a single heterozygous point mutation substituting Gln for Arg1174. Insulin binding capacity and affinity to Epstein-Barr virus transformed lymphocytes was normal. Quantitative analysis of autophosphorylation and subs...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)00876-0
更新日期:1994-09-05 00:00:00
abstract::We have studied the incorporation of [(14)C]serine and of [(3)H]sphingosine into sphingomyelin in the presence or absence of brefeldin A (BFA) in three different cell types. Administration of BFA (1 microgram/ml) to fibroblasts for 24 h increased the incorporation of label into sphingomyelin 1.5-3 fold compared with u...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)01818-4
更新日期:2000-07-28 00:00:00
abstract::The Hippo-Warts pathway defines a novel signaling cascade involved in organ size control and tumor suppression. However, the developmental function of this pathway is less understood. Here we report that the Caenorhabditis elegans homolog of Warts, Ce-wts-1, plays important roles during worm development. The null alle...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2009.09.002
更新日期:2009-10-06 00:00:00
abstract::Low density lipoproteins are highly sensitive to oxidation by copper salts, and such peroxidation is accompanied by macrophage scavenger receptor recognition. This study shows that fresh human atherosclerotic material (aneurysms and endarterectomies) can contain detectable amounts of redox active iron and copper that ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)00726-p
更新日期:1995-07-24 00:00:00
abstract::Protein deglutathionylation is mainly catalyzed by glutaredoxins (GRXs). We have analyzed the biochemical properties of four of the six different GRXs of Chlamydomonas reinhardtii. Kinetic parameters were determined for disulfide and dehydroascorbate reduction but also for deglutathionylation of artificial and protein...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2010.04.034
更新日期:2010-06-03 00:00:00
abstract::We have previously published a simple kinetic model to analyse possible pitfalls in kinetic measurements of H+/O ratios in mitochondria [(1984) FEBS Lett. 178, 187-192]. While this model demonstrated how relative electrode response times may affect the results, it did not adequately describe the kinetics of proton bac...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80608-1
更新日期:1986-06-09 00:00:00
abstract::Ataxin-3, which is encoded by a gene that has been associated with Machado-Joseph disease, contains a catalytic N-terminal Josephin domain with deubiquitinase activity. Here, we show that the Josephin domain of ataxin 3 catalyzes endo-type cleavage of Lys48-linked polyubiquitin. Furthermore, NMR data obtained followin...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2014.10.013
更新日期:2014-11-28 00:00:00
abstract::The mitochondrial alternative oxidase is a diiron carboxylate quinol oxidase (Dox) found in plants and some fungi and protists, but not animals. The plastid terminal oxidase is distantly related to alternative oxidase and is most likely also a Dox protein. Database searches revealed that the alpha-proteobacterium Novo...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(03)01309-7
更新日期:2003-12-18 00:00:00
abstract::Misfolded or unassembled secretory proteins are retained in the endoplasmic reticulum (ER) and subsequently degraded by the cytosolic ubiquitin-proteasome system. This requires their retrograde transport from the ER lumen into the cytosol, which is mediated by the Sec61 translocon. It had remained a mystery whether ER...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)01724-4
更新日期:1999-01-29 00:00:00
abstract::We established a medium-scale real-time RT-PCR system focusing on transcription factors and applied it to their expression profiles in the adult mouse 11 brain regions (http://genome.gsc.riken.jp/qRT-PCR/). Almost 90% of the examined genes showed significant expression in at least one region. We successfully extracted...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2004.07.068
更新日期:2004-08-27 00:00:00
abstract::Phosphate triester derivatives of the anti-neoplastic alkyl lyso phospholipid (ALP) have been prepared as novel potential therapeutic agents. In particular, symmetrical phosphate triesters have been prepared, using phosphorochloridate chemistry. The compounds have been fully characterised by a range of techniques, and...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)00959-d
更新日期:1995-09-25 00:00:00
abstract::Apoptosis specific proteins (ASP) are expressed in the cytoplasm of cultured mammalian cells of various lineages following induction of apoptosis. The cDNA encoding ASP has been cloned from a human expression library and has significant homology to the Saccharomyces cerevisiae APG5 gene which is essential for yeast au...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)00266-x
更新日期:1998-04-03 00:00:00
abstract::hTrp3 is a human homologue of the Drosophila gene responsible for a transient receptor potential (trp) mutation. When stably expressed in HEK293 cells, hTrp3 formed ion channels that were active under resting conditions but could be further stimulated by carbachol or ATP via endogenous muscarinic or purinergic recepto...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)00035-0
更新日期:1998-02-06 00:00:00
abstract::Partial amino acid sequence was obtained from the massive myofibrillar protein nebulin. This consists of repeating motifs of about 35 residues and super-repeats of 7 x 35 = 245 residues. The repeat-motifs are likely to be largely alpha-helical and to interact with both actin and tropomyosin in thin filaments. Nebulin ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80503-u
更新日期:1991-05-06 00:00:00
abstract::The selectively red excited emission spectrum, at room temperature, of the in vitro reconstituted Lhca4, has a pronounced non-equilibrium distribution, leading to enhanced emission from the directly excited low-energy pigments. Two different emitting forms (or states), with maximal emission at 713 and 735nm (F713 and ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.02.057
更新日期:2005-04-11 00:00:00
abstract::We have isolated a portion of the canine gene encoding the orphan receptor RDC1 [1]. The complete coding sequence is contained in a single exon, and an intron divides the 5' untranslated region of RDC1 mRNA. The RDC1 protein is 94% homologous to the gene product of GPRN1, which has been proposed to serve as a VIP rece...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80184-i
更新日期:1992-03-30 00:00:00
abstract::We have determined the first de novo position of the secondary quinone QB in the Rhodobacter sphaeroides reaction center (RC) using phases derived by the single wavelength anomalous dispersion method from crystals with selenomethionine substitution. We found that in frozen RC crystals, QB occupies primarily the proxim...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2004.06.042
更新日期:2004-07-16 00:00:00
abstract::The concentration of caffeine required to elute wholly single-stranded DNA from benzoylated DEAE-cellulose is proportional to the polynucleotide length. The use of benzoylated DEAE-cellulose chromatography for isolating and sizing single-stranded regions in double-stranded DNA has been examined using a series of hybri...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)80003-6
更新日期:1988-02-15 00:00:00
abstract::The purpose of this investigation was to study the effect on cap structure for trans-splicing in mammalian cells. The early SV40 Bst/Bam pre-mRNA (cRNA) was synthesized in vitro in both capped (cap-Bst/Bam-cRNA) and non-capped (Bst/Bam-cRNA) versions and microinjected into the nuclei of TC7 cells. Trans-splicing was m...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)00956-8
更新日期:1996-09-30 00:00:00
abstract::Cloned mouse delta-subtype opioid receptor (DOR1) was expressed in Xenopus oocytes to study the signal transduction. Opioid delta-agonists evoked a calcium-dependent chloride current in oocytes injected with mRNA derived from DOR1, together with that from the alpha subunit of Gi1. The delta-agonist-induced current was...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)80677-m
更新日期:1993-11-01 00:00:00
abstract::To characterize the proteins involved in cell clump/cell adhesion of insect cellular defense reactions, we induced the cell clump/cell adhesion reaction in vitro with the hemolymph of larvae of the coleopteran insect, Tenebrio molitor. The 72 kDa protein was specifically enriched in the residues of cell clump/cell adh...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00067-8
更新日期:1999-02-12 00:00:00
abstract::Thrombomodulin (TM) is a single-transmembrane glycoprotein receptor for thrombin, which is best known as a cofactor for thrombin-mediated activation of anticoagulant protein C. C-type lectin-like domain (CTLD) of TM has distinct coagulation/fibrinolysis-independent anti-inflammatory properties. Here we found anti-infl...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2011.10.002
更新日期:2011-11-04 00:00:00
abstract::Uncoupling protein 3 (UCP3) may reduce mitochondrial ROS production, and thereby protect against mitochondrial dysfunction in skeletal muscle. UCP3 has been suggested to specifically fulfill this role under high-fat conditions. Here we show that UCP3 knockout mice indeed have elevated mitochondrial ROS production afte...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2011.11.012
更新日期:2011-12-15 00:00:00
abstract::The sequence of seminalplasmin, a basic antimicrobial and transcription-inhibitory protein from bovine seminal plasma, has been determined using an automated sequenator. This sequence is slightly different from that reported earlier by Theil and Scheit [(1983) EMBO J. 2, 1159-1163] and identical with that of caltrin, ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80615-9
更新日期:1986-06-09 00:00:00
abstract::The aim of the present study is to determine the role of intracellular Ca(2+) in VEGF signaling. We demonstrate that reduction in Ca(2+) by chelating compound BAPTA-AM or by IP(3)-endoplasmic reticulum blocker 2-APB selectively inhibited VEGF-induced activation of c-Src-PI3K-Akt but not ERK1/2 in human coronary artery...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2010.05.053
更新日期:2010-07-16 00:00:00
abstract::Disulfide bridges were introduced into CrylAa, a Bacillus thuringiensis lepidopteran toxin, to stabilize different protein domains including domain I alpha-helical regions thought to be involved in membrane integration and permeation. Bridged mutants could not form functional ion channels in lipid bilayers in the oxid...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)00626-1
更新日期:1997-06-30 00:00:00
abstract::The kinetics of complexing inactivation at identical enzyme and inhibitor concentrations were analyzed and the equations of product generation were derived when the free enzyme concentration is great, larger or smaller than the dissociation constant of inhibitor, K(I). The kinetic constants can be obtained by fitting ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)00771-0
更新日期:1997-08-04 00:00:00