Abstract:
:We have previously published a simple kinetic model to analyse possible pitfalls in kinetic measurements of H+/O ratios in mitochondria [(1984) FEBS Lett. 178, 187-192]. While this model demonstrated how relative electrode response times may affect the results, it did not adequately describe the kinetics of proton back-diffusion across the membrane. Here this model is further developed and improved, and shown to give a good quantitative description of both oxygen-pulse type experiments as well as of experiments where the reaction is started by photolysis of the cytochrome c oxidase-CO complex. Simulations based on this model reveal that the extrapolation procedure used by Lehninger et al. [e.g. (1984) J. Biol. Chem. 259, 4802-4811] to estimate the H+/O ratio will tend to yield overestimated values. This is mainly due to the back-diffusion of protons into the mitochondria, which is not correctly accounted for by this extrapolation.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Krab K,Wikström Mdoi
10.1016/0014-5793(86)80608-1subject
Has Abstractpub_date
1986-06-09 00:00:00pages
198-204issue
2eissn
0014-5793issn
1873-3468pii
0014-5793(86)80608-1journal_volume
201pub_type
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