Receptor phage. Display of functional domains of the human high affinity IgE receptor on the M13 phage surface.

Abstract:

:In this paper we demonstrate that phage display technology is a suitable system for studying the interaction between the high-affinity receptor for IgE (Fc epsilon RI) and IgE. The alpha subunit extracellular domains of the human receptor were expressed on the surface of filamentous phage M13 fused to the carboxyl-terminal part of the gene III protein (pIII). Two constructs were made, the first with both the Ig-like domains of the receptor alpha chain and the second with only the C-terminal domain. The fusion genes were cloned in a phagemid vector to display monovalently the receptor on the phage surface. Our results indicate that the alpha receptor expressed on the phage is able to interact with IgE as demonstrated by an ELISA assay. In addition, by using the same system, we show that a single domain of the alpha receptor is sufficient for the interaction with IgE although with a binding affinity lower than that of the two-domain receptor.

journal_name

FEBS Lett

journal_title

FEBS letters

authors

Scarselli E,Esposito G,Traboni C

doi

10.1016/0014-5793(93)80226-k

subject

Has Abstract

pub_date

1993-08-23 00:00:00

pages

223-6

issue

1-2

eissn

0014-5793

issn

1873-3468

pii

0014-5793(93)80226-K

journal_volume

329

pub_type

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